Red blood cell membrane water permeability increases with length of ex vivo storage

Alshalani, Abdulrahman; Acker, Jason P.

doi:10.1016/j.cryobiol.2017.04.009

Cited by 14 publications

(9 citation statements)

References 45 publications

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“…The erythrocyte membrane permeability to water is known to be dependent not only on individual characteristics of donor, techniques for erythrocyte procurement, but on storage term of cells prior to freezing as well [1,2]. The water transport through erythrocyte membrane is a complex process and it may occur in several ways: through lipid bilayer, UT-B, aquaporins 1 and 3 [13].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Impact of combined eﬀect of penetrating and non-penetrating medium components on post-hypertonic lysis development in human erythrocytes

Chabanenko

Орлова

Shpakova

2020

Probl Cryobiol Cryomed

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Реферат: Досліджували постгіпертонічний лізис еритроцитів людини при варіюванні складу середовища на етапі дегідратації. Осмоляльність комбінованих середовищ, які містять різні концентрації гліцерину і NaCl, становила ~ 2370 мOсм/л. Показано, що рівень постгіпертонічного лізису еритроцитів визначається не загальною осмоляльністю середовища дегідратації, а концентрацією гліцерину в складі комбінованих середовищ. Встановлено, що характер розвитку постгіпертонічного лізису еритроцитів за використання гліцерину в складі комбінованих середовищ на етапі дегідратації не залежить від попереднього насичення клітин кріопротектором. Порівняльне вивчення постгіпертонічного шоку еритроцитів за 37 і 0°С показало, що в останньому випадку гемоліз клітин розвивається за умов використання гліцерину в більш низькій концентрації (10%) у складі комбінованих середовищ (при 0°С рівень постгіпертонічного лізису еритроцитів вище в 2,7 раза). Встановлений ефект може бути пов'язаний зі значною різницею в швидкостях транспорту води і кріопротектору через еритроцитарну мембрану при низькій температурі. Ключові слова: постгіпертонічний шок, постгіпертонічний лізис, комбіновані середовища, гліцерин, еритроцити.

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Section: Resultsmentioning

confidence: 99%

“…Межидов та співавт. [1] пов'язують із впливом внутрішньоклітинної концентрації білка (переважно представленого гемоглобіном) на проникність еритроцитарної мембрани для гліцерину.…”

Section: таблицяunclassified

Impact of combined eﬀect of penetrating and non-penetrating medium components on post-hypertonic lysis development in human erythrocytes

Chabanenko

Орлова

Shpakova

2020

Probl Cryobiol Cryomed

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“…As RBC units age under refrigerated conditions, a well‐described “storage lesion(s)” develops. The RBC storage lesions are characterized by altered RBC morphology, rheologic changes, metabolic derangements, changes in oxygen affinity, changes in osmotic regulation, and changes in the ability to vasoregulate . In addition, RBC hemolysis (both during storage and after transfusion) can lead to reduced pH, increased lactate and other metabolic wastes, and release of microparticles as well as accumulation of cell‐free hemoglobin (Hb), heme, and iron .…”

Section: Proposed Mechanismsmentioning

confidence: 99%

“…The RBC storage lesions are characterized by altered RBC morphology, rheologic changes, metabolic derangements, changes in oxygen affinity, changes in osmotic regulation, and changes in the ability to vasoregulate. [78][79][80][81][82][83][84][85] In addition, RBC hemolysis (both during storage and after transfusion) can lead to reduced pH, increased lactate and other metabolic wastes, and release of microparticles as well as accumulation of cell-free hemoglobin (Hb), heme, and iron. 26,78,86-90 Iron content can be in the form of transferrin-bound iron, non-transferrin-bound iron (NTBI), or labile plasma iron.…”

Section: Rbc Storage Lesion and Decompartmentalized Rbc Contentsmentioning

confidence: 99%

Mechanisms of red blood cell transfusion‐related immunomodulation

et al. 2018

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Red blood cell (RBC) transfusion is common in critically ill, postsurgical, and posttrauma patients in whom both systemic inflammation and immune suppression are associated with adverse outcomes. RBC products contain a multitude of immunomodulatory mediators that interact with and alter immune cell function. These interactions can lead to both proinflammatory and immunosuppressive effects. Defining clinical outcomes related to immunomodulatory effects of RBCs in transfused patients remains a challenge, likely due to complex interactions between individual blood product characteristics and patient-specific risk factors. Unpacking these complexities requires an in-depth understanding of the mechanisms of immunomodulatory effects of RBC products. In this review, we outline and classify potential mediators of RBC transfusion-related immunomodulation and provide suggestions for future research directions.

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“…температур, однако для обеспечения сохранности биообъекта при замораживании необходимо введение в него соединений с криопротекторными свойствами [2,3,5]. В многочисленных отечественных и зарубежных исследованиях показано, что они оказывают неодинаковое действие на параметры плазмы и форменные элементы крови [2,4,[6][7][8]. Это позволяет говорить о возможности индивидуализации подбора криоконсервирующих агентов на основании предварительного анализа биологической жидкости реципиента [2,8].…”

Section: Introductionunclassified

Experimental Evaluation of Cryoprotector Systemic Effects

Мартусевич

Костяев

Kovaleva

2019

Kuban. nauсh. med. vestnik

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Aim. Experimental evaluation of the effect of some cryoprotectors on crystallogenic properties of mice blood serum at intraperitoneal administration.Material and methods. The experiment was performed on 40 adult white line mice divided into 4 equal groups. Animals from the control (fi rst) group received a single intraperitoneal injection of saline (0.5 ml agent/animal), animals of the second-fourth groups — 3 % solution of glycerin, dimethyl sulphoxide and trombocryodmac (N, N-dimethylacetamide), respectively, in the same quantity.Results. It is shown that single intraperitoneal introduction of cryoprotectants (solutions of glycerin, dimethyl sulphoxide and trombocryodmac) has a systemic effect on the mouse organism, which is manifested through the changes of crystallogenic properties of blood serum. 3% solution of glycerin, dimethyl sulphoxide and trombocryodmac have multidirectional infl uence on crystallogenic properties of mouse blood serum. The most pronounced physiological effect was established for dimethyl sulphoxide.Conclusion. Our studies demonstrate the presence of systemic effects in cryopreservative substances. It was confi rmed by the changes of crystallogenic properties of mice blood serum under intraperitoneal administration of cryopreservatives.

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Red blood cell membrane water permeability increases with length of ex vivo storage

Cited by 14 publications

References 45 publications

Impact of combined eﬀect of penetrating and non-penetrating medium components on post-hypertonic lysis development in human erythrocytes

Impact of combined eﬀect of penetrating and non-penetrating medium components on post-hypertonic lysis development in human erythrocytes

Mechanisms of red blood cell transfusion‐related immunomodulation

Experimental Evaluation of Cryoprotector Systemic Effects

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