Red blood cell membrane-coated upconversion nanoparticles for pretargeted multimodality imaging of triple-negative breast cancer

Li, Mengting; Fang, Hanyi; Liu, Qingyao; Gai, Yongkang; Yuan, Lei; Wang, Sheng; Li, Huiling; Hou, Yi; Gao, Mingyuan; Lan, Xiaoli

doi:10.1039/d0bm00029a

Cited by 81 publications

(57 citation statements)

References 51 publications

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“…Constructs that employ RBC membranes as a substrate coating have also been shown to be stable, with many constructs showing minimal changes in size after storage of up to one week [ 42 , 43 ]. We have demonstrated that after 12 h of storage in the dark at 4 and 37 °C, fluorescence emission of nEGs loaded with ICG (ICG-nEGs) was retained, whereas there was nearly a 40% reduction in the emission for free ICG [ 44 ].…”

Section: Fabrication Of Rbc-derived Constructsmentioning

confidence: 99%

“…Multiple groups have investigated combining UCNPs with RBC-based delivery constructs, particularly for cancer imaging. For example, Li et al prepared NaGdF 4 :Yb, Tm UCNPs that were then coated with RBC membranes [ 42 ]. The resulting constructs showed increased tumor uptake compared to bare UCNPS, and could be used for UCL, as well as magnetic resonance imaging (MRI) and positron emission tomography (PET) imaging [ 42 ].…”

Section: Potential Clinical Applicationsmentioning

confidence: 99%

“…For example, Li et al prepared NaGdF 4 :Yb, Tm UCNPs that were then coated with RBC membranes [ 42 ]. The resulting constructs showed increased tumor uptake compared to bare UCNPS, and could be used for UCL, as well as magnetic resonance imaging (MRI) and positron emission tomography (PET) imaging [ 42 ]. Similarly, Rao et al prepared β-NaYF 4 :Er 3+ , Yb 3+ UCNPs coated with RBC membranes and reported increased tumor uptake of the resulting constructs compared to bare UCNPs [ 36 ].…”

Section: Potential Clinical Applicationsmentioning

confidence: 99%

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Phototheranostics Using Erythrocyte-Based Particles

et al. 2021

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There has been a recent increase in the development of delivery systems based on red blood cells (RBCs) for light-mediated imaging and therapeutic applications. These constructs are able to take advantage of the immune evasion properties of the RBC, while the addition of an optical cargo allows the particles to be activated by light for a number of promising applications. Here, we review some of the common fabrication methods to engineer these constructs. We also present some of the current light-based applications with potential for clinical translation, and offer some insight into future directions in this exciting field.

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Section: Fabrication Of Rbc-derived Constructsmentioning

confidence: 99%

Section: Potential Clinical Applicationsmentioning

confidence: 99%

Section: Potential Clinical Applicationsmentioning

confidence: 99%

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Phototheranostics Using Erythrocyte-Based Particles

et al. 2021

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“…In a different effort, upconverting NPs (NaGdF 4 :Yb,Tm) coated with RBC membranes and an incorporated linker for click chemistry were accumulated in a tumor site in a mouse model. [18] Then, modified PET agent 18 F was injected and attached to the preaccumulated NPs via click chemistry. A PET signal from the tumor site was detectable after 30 min, indicating the feasibility of the approach.…”

Section: Non-nuclei Containing Cells Sourcesmentioning

confidence: 99%

Cell Membrane Coated Particles

Spanjers

Städler

2020

Adv. Biosys.

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that red blood cells (RBCs) circulate over 100 days although they are larger than the smallest capillaries. Nature not only employs the surface chemistry of the cells, but also their geometry and mechanical properties, i.e., the biconcave shape of the RBCs, give them the deformability to pass capillaries. Therefore, efforts to mimic RBCs are being reported from fabricating geometrically matching entities to matching their chemical composition. [2] However, making an artificial copy of a cell is challenging. Hence, the compromise between relying on a single interaction point (typically receptor-antibody interactions) of nanoformulations with cells and tissue, and the great complexity of an entire cell is currently a more realistic approach. An interesting example in this context is the use of purified cell membrane to camouflage (nano)particles. This concept offers opportunity to mimic cell−cell interactions employing multiple types of contact points while avoiding the need to obtain/purify specific proteins. This nature-inspired approach was first shown in 2011 with RBC membranes to enhance particle blood circulation times and developed in the next 4-5 years to include white blood cells, platelets, and cancerous cell sources. [3] The field gained increasing attention over the past years, expanding on types of cell sources, coated particles, and envisioned applications. This progress report will highlight selected efforts in the field from the past 2-3 years focusing on mammalian cell sources and coating of colloidal systems (Scheme 1). Efforts pre-dating 2017/2018 were already discussed in detail in several reviews. [4] First, a short overview over the general cell membrane purification procedure and subsequent characterization will be provided. Then, we will outline advances made when non-nuclei or nuclei containing cells sources were used. Finally, coatings consisting of two types of cell membrane (hybrid coatings) will be discussed 2. Formation and Characterization of Cell Membrane Coated Particles Despite of the variation in cell sources, the general purification methods of cell membranes and sequential coating of particles are rather similar and therefore described only briefly. Furthermore, important characterization techniques for size, protein content, and surface charge are discussed. We would like to note that there are a variety of other techniques not mentioned here specifically that can provide valuable specific information such as Fourier transform infrared spectroscopy for analysis of Nanoformulations are widely considered in the biomedical field for drug delivery, imaging, or detoxification purposes. Cell membrane coatings are a growing concept that aims to camouflage nanomaterials. The cell membranes are the first point of contact for cells to other biological or synthetic materials and nature has established signaling pathways in this context. In contrast to using purified membrane associated proteins, the use of purified cell membranes contains the protein of interest in a very native environme...

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“…Immune evasion and speci c tumor-targeting characteristics remain challenges to tackle to maximize the e cacy of PDT. Our previous work revealed that cancer cell membranes (CCm) might possess the desired immune evasion and homologous targeting characteristics [18,19]. Here, we designed the biomimetic oxygen delivery nanoprobe, namely cancer cell membrane-coated HSA-ICG-doped per uorotributylamine (CCm-HSA-ICG-PFTBA) for homologous targeting and hypoxia relieving at tumor sites.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Oxygen Delivery Nanoparticles for Enhancing Photodynamic Therapy in Triple-negative Breast Cancer

Fang

Gai

Wang

et al. 2021

Preprint

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Background Triple-negative breast cancer (TNBC) is a kind of aggressive breast cancer with a high rate of metastasis, poor overall survival time, and a low response to targeted therapies. To improve the therapeutic efficacy and overcome the drug resistance of TNBC treatments, here we developed the cancer cell membrane-coated oxygen delivery nanoprobe, CCm-HSA-ICG-PFTBA, which can improve the hypoxia at tumor sites and enhance the therapeutic efficacy of the photodynamic therapy (PDT), resulting in relieving the tumor growth in TNBC xenografts. Results The size of the CCm-HSA-ICG-PFTBA was 131.3 ± 1.08 nm. The in vitro 1O2 and ROS concentrations of the CCm-HSA-ICG-PFTBA group were both significantly higher than those of the other groups (P < 0.001). In vivo fluorescence imaging revealed that the best time window was at 24 h post-injection of the CCm-HSA-ICG-PFTBA. Both in vivo 18F-FMISO PET imaging and ex vivo immunofluorescence staining results exhibited that the tumor hypoxia was significantly improved at 24 h post-injection of the CCm-HSA-ICG-PFTBA. For in vivo PDT treatment, the tumor volume and weight of the CCm-HSA-ICG-PFTBA with NIR group were both the smallest among all the groups and significantly decreased compared to the untreated group (P < 0.01). No obvious biotoxicity was observed by the injection of CCm-HSA-ICG-PFTBA till 14 days. Conclusions By using the high oxygen solubility of perfluorocarbon (PFC) and the homologous targeting ability of cancer cell membranes, CCm-HSA-ICG-PFTBA can target tumor tissues, mitigate the hypoxia of the tumor microenvironment, and enhance the PDT efficacy in TNBC xenografts. Furthermore, the HSA, ICG, and PFC are all FDA-approved materials, which render the nanoparticles highly biocompatible and enhance the potential for clinical translation in the treatment of TNBC patients.

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Red blood cell membrane-coated upconversion nanoparticles for pretargeted multimodality imaging of triple-negative breast cancer

Abstract: Upconversion nanoparticles (UCNPs) have been widely employed for tumor imaging using magnetic resonance imaging (MRI) and upconversion luminescence (UCL) imaging.

Cited by 81 publications

References 51 publications

Phototheranostics Using Erythrocyte-Based Particles

Phototheranostics Using Erythrocyte-Based Particles

Cell Membrane Coated Particles

Biomimetic Oxygen Delivery Nanoparticles for Enhancing Photodynamic Therapy in Triple-negative Breast Cancer

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