Red blood cell distribution width – a strong prognostic marker in cardiovascular disease – is associated with cholesterol content of erythrocyte membrane

Tziakas, Dimitrios; Chalikias, Georgios K.; Grapsa, Anastasia; Gioka, Theodora; Tentes, Ioannis; Konstantinides, Stavros

doi:10.3233/ch-2012-1530

Cited by 63 publications

(35 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Erythrocyte is the unique cell for delivering oxygen to the body tissues. Its damage may cause a series of symptoms of tissue hypoxia (Tziakas et al, 2012). It has been shown that other adjuvant anti-cancer medicines, such as gambogic acid and tanshinone IIA, impaired erythrocytes via shrinking, reduction of ATP, and promotion of apoptosis (Lupescu et al, 2012;Zelenak et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes

Hao

Chen

et al. 2017

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Marsdeniae tenacissimae extract (MTE) has been used as an adjuvant medicine for cancer therapy for a long time. Although massive studies demonstrated its considerable anti-cancer effect, there is no research on its influence on erythrocytes, which are firstly interacted with MTE in the circulation. To investigate the influence of MTE on erythrocytes, we used a flow cytometer to detect the MTE-treated alternations of morphology, calcium concentration, and reactive oxygen species (ROS) level in erythrocytes. We used hemolysis under different osmotic solutions to evaluate the fragility of erythrocytes. Data showed that MTE treatment dose-dependently increased the ratio of erythrocyte fragmentation (P<0.001) and shrinking, and elevated the forward scatter (FSC) value (P<0.001) and calcium accumulation (P<0.001). MTE induced ROS production of erythrocytes under the high glucose condition (P<0.01) and consequently caused a rise in fragility (P<0.05). These results suggest that MTE induces cytotoxicity and aging in erythrocytes in a dose-dependent manner, and presents the possibility of impairment on cancer patients' circulating erythrocytes when MTE is used as an anti-cancer adjuvant medicine.

show abstract

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes

Hao

Chen

et al. 2017

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

show abstract

“…RBCs seem to have a high degree of uniformity but display certain variability in their hemoglobin F content, volume, shape and peripheral tissue oxygenation. However, major deviations from this normal range of variability are usually associated with pathophysiological conditions like hematuria [3], sickle cell anemia [4] or cardiovascular diseases [5].…”

Section: Introductionmentioning

confidence: 99%

Morphologically Homogeneous Red Blood Cells Present a Heterogeneous Response to Hormonal Stimulation

et al. 2013

View full text Add to dashboard Cite

Red blood cells (RBCs) are among the most intensively studied cells in natural history, elucidating numerous principles and ground-breaking knowledge in cell biology. Morphologically, RBCs are largely homogeneous, and most of the functional studies have been performed on large populations of cells, masking putative cellular variations. We studied human and mouse RBCs by live-cell video imaging, which allowed single cells to be followed over time. In particular we analysed functional responses to hormonal stimulation with lysophosphatidic acid (LPA), a signalling molecule occurring in blood plasma, with the Ca2+ sensor Fluo-4. Additionally, we developed an approach for analysing the Ca2+ responses of RBCs that allowed the quantitative characterization of single-cell signals. In RBCs, the LPA-induced Ca2+ influx showed substantial diversity in both kinetics and amplitude. Also the age-classification was determined for each particular RBC and consecutively analysed. While reticulocytes lack a Ca2+ response to LPA stimulation, old RBCs approaching clearance generated robust LPA-induced signals, which still displayed broad heterogeneity. Observing phospatidylserine exposure as an effector mechanism of intracellular Ca2+ revealed an even increased heterogeneity of RBC responses. The functional diversity of RBCs needs to be taken into account in future studies, which will increasingly require single-cell analysis approaches. The identified heterogeneity in RBC responses is important for the basic understanding of RBC signalling and their contribution to numerous diseases, especially with respect to Ca2+ influx and the associated pro-thrombotic activity.

show abstract

“…Considering that EMC content is believed to be directly correlated with plasma cholesterol level, lower TC levels we report here is consistent with the observed EMC results in our study. Although the mechanism underlying the correlation between EMC and plasma lipoprotein levels remains unclear, it has been suggested that the exchange rate of cholesterol and phospholipids between erythrocyte and lipoproteins is regulated by several factors involving the number and distance of transfer sites among these particles as well as differences in C/P ratios of donor and acceptor particles (Rothblat and Phillips 2010 ; Tziakas et al 2008 , 2012 ). In particular, sphingomyelin content and lecithin and cholesterol acyltransferase activity of erythrocyte membrane regulate the rate of transfer of free cholesterol between cell membrane and plasma lipoproteins (Tziakas et al 2010 ).…”

Section: Discussionmentioning

confidence: 99%

“…Although erythrocyte membrane cholesterol (EMC) content is correlated with plasma lipid composition, the mechanism concerning a possible interaction of EMC with the lipoprotein subfractions is not yet clear. Recent reports indicate that EMC constitutes a substantial bulk of the cholesterol in lipid-rich core regions of atherosclerotic plaques and it might serve as a valuable marker for cardiovascular events (Tziakas et al 2008 , 2010 , 2012 ; Vaya et al 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Cholesterol Forms and Traditional Lipid Profile for Projection of Atherogenic Dyslipidemia: Lipoprotein Subfractions and Erythrocyte Membrane Cholesterol

et al. 2013

View full text Add to dashboard Cite

Atherogenic dyslipidemia characterized by abnormal changes in plasma lipid profile such as low high-density lipoprotein (HDL) and increased triglyceride (TG) levels is strongly associated with atherosclerotic diseases. We aimed to evaluate the levels of pro- and antiatherogenic lipids and erythrocyte membrane cholesterol (EMC) content in normo- and dyslipidemic subjects to investigate whether EMC content could be a useful marker for clinical presentation of atherogenic dyslipidemia. Low-density lipoprotein (LDL), HDL and their subfraction levels and erythrocyte lipid content were determined in 64 normolipidemic (NLs), 42 hypercholesterolemic (HCs) and 42 mixed-type dyslipidemic subjects (MTDs). Plasma atherogenic lipid indices [small–dense LDL (sdLDL)/less-dense HDL (LHDL), TC/HDL-C, TG/HDL-C and Apo B/AI] were higher in MTDs compared to NLs (p < 0.001). The highest sdLDL level was observed in HCs (p < 0.01). Despite a slight increase in EMC level in dyslipidemic subgroups, the difference was not statistically significant. A significant negative correlation, however, was observed between EMC and sdLDL/LHDL in HCs (p < 0.035, r = −0.386). Receiver operating characteristic curves to predict sdLDL level showed that TG and EMC levels had higher area under curve values compared to other parameters in HCs. We showed that diameters of larger LDL and HDL particles tend to shift toward smaller values in MTDs. Our results suggest that EMC content and TG levels may be a useful predictor for sdLDL level in hypercholesterolemic patients.

show abstract

Red blood cell distribution width – a strong prognostic marker in cardiovascular disease – is associated with cholesterol content of erythrocyte membrane

Cited by 63 publications

References 15 publications

Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes

Cytotoxicity of anti-tumor herbal Marsdeniae tenacissimae extract on erythrocytes

Morphologically Homogeneous Red Blood Cells Present a Heterogeneous Response to Hormonal Stimulation

Cholesterol Forms and Traditional Lipid Profile for Projection of Atherogenic Dyslipidemia: Lipoprotein Subfractions and Erythrocyte Membrane Cholesterol

Contact Info

Product

Resources

About