Red blood cell deformability and aggregation, cell adhesion molecules, oxidative stress and nitric oxide markers after a short term, submaximal, exercise in sickle cell trait carriers

Tripette, Julien; Connes, Philippe; Beltan, Eric; Chalabi, Tawfik; Marlin, Laurent; Chout, R.; Başkurt, Oğuz K.; Hue, Olivier; Hardy‐Dessources, Marie‐Dominique

doi:10.3233/ch-2010-1325

Cited by 40 publications

(24 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although, the increased WBC count did not impact on the whole ηb, one could suggest the presence of a greater risk for vaso‐occlusive event after exercise because WBC are highly involved in the pathophysiological mechanisms of vaso‐occlusion (Okpala, 2006). However, the 10% WBC increase observed in SCA patients is much smaller than the increase observed in healthy subjects (+43%) or sickle cell trait carriers (+47%) after a 15‐min moderate exercise performed slightly above the first ventilatory threshold (Tripette et al , 2010). It is important to note that no subject experienced vaso‐occlusive crisis or complained of any pain during and after exercise and within the 3 d of follow‐up.…”

Section: Discussionmentioning

confidence: 77%

“…This approach has already been extensively used in asymptomatic heterozygous carriers (sickle cell trait carriers) to better understand the putative causes of exercise‐related adverse events in that population (Connes et al , 2008). Most of the studies performed in sickle cell trait carriers demonstrated an abnormal response of blood rheology during prolonged intense endurance exercise or strenuous exercise, such as a large increase in blood viscosity (Tripette et al , 2007, 2010) that could participate in microcirculatory impairment (Connes, 2010). Given that exercise intensity may be very problematic in SCA patients, the exercise intensity was set at 43 ± 6 W and can be classified as mild to moderate (Lonsdorfer et al , 1983).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Mild haemorheological changes induced by a moderate endurance exercise in patients with sickle cell anaemia

et al. 2011

Self Cite

View full text Add to dashboard Cite

The levels and duration of physical activity that can be considered as completely safe in patients with sickle cell anaemia (SCA) is unknown. The present study compared the haemorheological and haematological profile, cell density distribution and basic biochemistry between a group of 17 patients with SCA and 21 healthy subjects before and after a 20 min duration submaximal cycling exercise at the same absolute workload. Blood was sampled at rest and 3 min after the end of exercise for measurement of biological parameters. Exercise did not affect the haematocrit and blood viscosity in the two groups. Plasma viscosity was not different between the two groups at rest and similarly increased with exercise. The proportion of intermediary dense cells (with density between 1·11 and 1·12 g/ml) decreased with exercise in the SCA group resulting in an increase in the proportion of red blood cells with a density >1·12 g/ml. No change was observed in the control group. The present study suggests that mild‐moderate exercise is not very harmful for SCA patients. The haemorheological and haematological changes were very mild, except for the formation of dense cells but no clinically significant signs of medical complication were present in any of the patients.

show abstract

Section: Discussionmentioning

confidence: 77%

mentioning

confidence: 99%

Mild haemorheological changes induced by a moderate endurance exercise in patients with sickle cell anaemia

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…In contrast to homozygous SCD, heterozygous SCT is traditionally regarded as a benign condition (13)(14)(15). However, during strenuous exercise, individuals can develop a syndrome resembling SCD, with vasoocclusive events resulting from changes in the RBC morphology, viscoelasticity, and adhesion (1,2,6,(16)(17)(18)(19). Vaso-occlusion occurs in crises in which the arterial circulation is blocked at one or many sites, leading to organ damage and ischemic pain (20).…”

Section: Introductionmentioning

confidence: 99%

Epinephrine Modulates BCAM/Lu and ICAM-4 Expression on the Sickle Cell Trait Red Blood Cell Membrane

Maciaszek

Andemariam

Huber

et al. 2012

Biophysical Journal

View full text Add to dashboard Cite

Collapse and sudden death in physical training are the most serious complications of sickle cell trait (SCT). There is evidence that erythrocytes in SCT patients aggregate during strenuous exercise, likely because of adhesive interactions with the extracellular matrix (ECM) and endothelial cells, and because of their irregular viscoelastic properties. This results in inflammation, blood flow impairment, and vaso-occlusive events. However, the exact role of stress conditions and how they lead to these complications is virtually unknown. Using single-molecule atomic force microscopy experiments, we found that epinephrine, a hormone that is secreted under stressful conditions, increases both the frequency and strength of adhesion events between basal cell adhesion molecule (BCAM/Lu) and ECM laminin, and between intercellular adhesion molecule-4 (ICAM-4) and endothelial α(v)β(3), compared with nonstimulated SCT erythrocytes. Increases in adhesion frequency provide significant evidence of the role of epinephrine in BCAM/Lu-laminin and ICAM-4-α(v)β(3) bonding, and suggest mechanisms of vaso-occlusion during physical exertion in SCT.

show abstract

“…[12][13][14] Indeed, low-shear microcirculatory flow is characteristic of SCD rheological behavior and is suggested to bear some association with a preponderance of oxidant-mediated etiology. [15][16][17][18][19][20] The inherent susceptibility of RBCs to oxidant stress coupled with the deoxygenationrelated increases in the membrane fragility of sickle RBCs could underlie some aspects of the abnormal flow profile seen in this condition. 15,17,19 In spite of these findings, the kinetics of RBC aggregation in SCD and its role on blood flow dynamics in vivo is still poorly understood.…”

mentioning

confidence: 99%

Erythrocyte Aggregation and Blood Viscosity is Similar in Homozygous Sickle Cell Disease Patients with and without Leg Ulcers

2018

View full text Add to dashboard Cite

There is no consensus regarding the role of red blood cell (RBC) aggregation in the pathogenesis of leg ulcers (LUs) in sickle cell disease (SCD). We sought to evaluate whether the cross-sectional determination of RBC aggregation and hematological indices were associated with the presence of LU in homozygous SCD. Twenty-seven patients with LU and 23 with no history of ulceration were recruited into the study. A laser-assisted rotational red cell analyzer (LoRRca) was used in the determination of the aggregation index (AI), aggregation half-time ( ), and the RBC aggregate strength (AMP). Hematological indices were determined using a CELL-DYN Ruby analyzer. Whole blood viscosity (WBV) and plasma viscosity (PV) were measured using a Vilastic bioprofiler. The data were presented as means ± standard deviation or median, interquartile range. Two-sample -test was used to test for associations between the AIs, WBV, and PV in patients with and without LU. Statistical significance was taken as < 0.05. All analyses were conducted using Stata/SE . 12.1 (StataCorp, College Station, TX). The AI was comparable in the group with and without ulcers (68.6, 16.7 versus 67.7, 16.9; = 0.74); (1.7, 1.3 versus 1.8, 1.3; = 0.71); AMP (18.8, 14.5 versus 19.1, 13.3; = 0.84), WBV (3.8, 1.2 versus 3.8, 0.7; = 0.77); and the PV (1.3, 0.08 versus 1.4, 0.1; = 0.31) and were also not statistically different between the groups of participants. RBC aggregation and aggregate strength are not associated with leg ulceration in SCD.

show abstract

Red blood cell deformability and aggregation, cell adhesion molecules, oxidative stress and nitric oxide markers after a short term, submaximal, exercise in sickle cell trait carriers

Cited by 40 publications

References 32 publications

Mild haemorheological changes induced by a moderate endurance exercise in patients with sickle cell anaemia

Mild haemorheological changes induced by a moderate endurance exercise in patients with sickle cell anaemia

Epinephrine Modulates BCAM/Lu and ICAM-4 Expression on the Sickle Cell Trait Red Blood Cell Membrane

Erythrocyte Aggregation and Blood Viscosity is Similar in Homozygous Sickle Cell Disease Patients with and without Leg Ulcers

Contact Info

Product

Resources

About