Red and White Cell Abnormalities in Chronic Renal Failure

Mansell, M. A.; Grimes, A. J.

doi:10.1111/j.1365-2141.1979.tb01121.x

Cited by 18 publications

(12 citation statements)

References 29 publications

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“…This is probably the main reason why a high intracellular ATP concentration observed in uremia is confined to erythrocytes. In white blood cells (which are known to contain all the enzymes necessary for the production of purines de novo and for all the incorversion and salvage pathways), ATP concentration is significantly reduced in CRF and the degree of the reduction parallels the severity of the renal impairment [7]. …”

Section: Discussionmentioning

confidence: 99%

“…The elevated concentration of ATP in erythrocytes of patients with CRF was observed by many authors [7, 8, 14, 15, 16, 17, 18], but the mechanisms leading to these abnormalities are obscure. Hyperphosphatemia and metabolic acidosis seem to be the most likely factors responsible for the increase of ATP concentration in these erythrocytes [8, 16, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28], although definitive evidence is still lacking.…”

Section: Introductionmentioning

confidence: 99%

“…A number of biochemical abnormalities observed in erythrocytes of chronically uremic patients could also be responsible for anemia: higher activity of glycolytic enzymes [3], increased rate of glucose consumption and lactate production [7, 8, 9], elevated concentration of 2,3-biphosphoglycerate [10], decreased Na + /K + -dependent membrane ATPase activity [11, 12], accumulation of unusual purine and pyrimidine nucleotides [6, 13]and raised ATP and GTP concentrations [7, 8, 14, 15]. …”

Section: Introductionmentioning

confidence: 99%

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Increased Rate of Adenine Incorporation into Adenine Nucleotide Pool in Erythrocytes of Patients with Chronic Renal Failure

Marlewski¹,

Smolenski²,

Szołkiewicz

et al. 2000

Nephron

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Background: Elevated purine nucleotide pool (mainly ATP) in erythrocytes of patients with chronic renal failure (CRF) is a known phenomenon, however the mechanism responsible for this abnormality is far from being clear. We hypothesize that the increased rate of adenine incorporation into adenine nucleotide pool is responsible for the elevated level of ATP in uremic erythrocytes. Methods: In chronically uremic patients we evaluated using HPLC technique: (a) plasma adenine concentration; (b) the rate of adenine incorporation into adenine nucleotide pool in uremic erythrocytes. Additionally, the effect of higher than physiological phosphate concentration (2.4 mM) and lower than physiological pH (7.1) on adenine incorporation into erythrocytes adenine nucleotide pool was investigated. Healthy volunteers with normal renal function served as control. Results: The concentration of adenine in plasma of CRF patients was found to be significantly higher than in plasma of healthy subjects. In contrast, adenosine concentration was similar both in healthy humans and in CRF patients. In isolated erythrocytes of uremic patients (incubated in the medium pH 7.4, containing 1.2 mM inorganic phosphate) adenine was incorporated into adenine nucleotide pool at a rate approximately 2-fold higher than in erythrocytes from healthy subjects. The rate of adenosine incorporation into adenine nucleotide pool was similar in erythrocytes of both studied groups. Incubation of erythrocytes obtained from healthy subjects in the medium pH 7.4, containing 2.4 mM inorganic phosphate, caused the increase of adenine incorporation into adenine nucleotide pool by about 60%. Incubation of the cells in the pH 7.1 buffer containing 2.4 mM inorganic phosphate increased the rate of adenine incorporation into adenylate approximately 2-fold as compared to erythrocytes incubated in the medium pH 7.4 containing 1.2 mM inorganic phosphate. Erythrocytes obtained from uremic patients and incubated in the pH 7.1 medium containing 2.4 mM phosphate incorporated adenine into adenine nucleotide pool at a rate similar to erythrocytes incubated in the medium pH 7.4 containing 1.2 mM phosphate. Erythrocytes obtained from either healthy subjects or from patients with CRF and incubated in the presence of higher than physiological concentration of inorganic phosphate (2.4 mM) and lower than physiological pH (7.1) did not exhibit any increase in the rate of adenisine incorporation into adenine nucleotide pool. Conclusion: These results suggest that the increased rate of adenine incorporation into adenine nucleotide pool could be partially responsible for the increased concentration of ATP in uremic erythrocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Increased Rate of Adenine Incorporation into Adenine Nucleotide Pool in Erythrocytes of Patients with Chronic Renal Failure

Marlewski¹,

Smolenski²,

Szołkiewicz

et al. 2000

Nephron

View full text Add to dashboard Cite

show abstract

“…The elevated concentration of ATP in erythrocytes from patients with chronic renal failure (CRF) has been observed by many authors [1, 2, 3, 4, 5, 6, 7], but the mechanism(s) leading to these abnormalities is still controversial. It is believed that hyperphosphatemia and metabolic acidosis triggering enhanced reutilization of purine bases are the factors responsible for the increased ATP concentration in uremic erythrocytes [1, 2, 5, 8, 9, 10], although definitive evidence is still lacking.…”

Section: Introductionmentioning

confidence: 99%

“…It is believed that hyperphosphatemia and metabolic acidosis triggering enhanced reutilization of purine bases are the factors responsible for the increased ATP concentration in uremic erythrocytes [1, 2, 5, 8, 9, 10], although definitive evidence is still lacking. Some authors suggest that plasma adenine could also induce the changes in erythrocyte adenine nucleotide concentration [11, 12].…”

Section: Introductionmentioning

confidence: 99%

High Plasma Adenine Concentration in Chronic Renal Failure and Its Relation to Erythrocyte ATP

Słomińska

Szołkiewicz

Smolenski

et al. 2002

Nephron

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Background: Concentration of plasma adenine has been found to increase in chronic renal failure (CRF). The aim of the present study was to evaluate whether high plasma adenine concentration contributes to the elevated ATP in erythrocytes of patients with CRF. Methods: Three groups of patients with CRF were studied: (A) 30 patients with different degree of CRF; (B) 11 patients on hemodialysis, and (C) 12 patients after successful renal transplantation. Concentrations of plasma adenine and erythrocyte adenine nucleotides were measured in groups A, B and C. Furthermore, adenine incorporation into erythrocyte adenine nucleotide pool was measured in group A. Results: A positive correlation between plasma adenine and creatinine concentrations was found in CRF as well as between plasma adenine and erythrocyte ATP. Furthermore, positive correlation was evident between the rate of adenine incorporation into erythrocyte adenine nucleotide pool and the severity of CRF. A significant reduction in both plasma adenine and erythrocyte ATP was observed immediately following hemodalysis, but 2 days later, high predialysis plasma adenine and erythrocyte ATP concentrations were restored. Following successful renal transplantation erythrocyte ATP and plasma adenine concentrations reached control values. Conclusion: Our results provide evidence that plasma adenine concentration increases in parallel to the progress of the disease and that it could be responsible for the increase in erythrocyte ATP of patients with CRF.

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Nucleotide Concentrations in Leucocytes and Their Use in Controlling the Quality of Cell Preparations

Harkness¹,

Coade²,

Mansell³

et al. 1982

Advances in Experimental Medicine and Biology

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Red and White Cell Abnormalities in Chronic Renal Failure

Cited by 18 publications

References 29 publications

Increased Rate of Adenine Incorporation into Adenine Nucleotide Pool in Erythrocytes of Patients with Chronic Renal Failure

Increased Rate of Adenine Incorporation into Adenine Nucleotide Pool in Erythrocytes of Patients with Chronic Renal Failure

High Plasma Adenine Concentration in Chronic Renal Failure and Its Relation to Erythrocyte ATP

Nucleotide Concentrations in Leucocytes and Their Use in Controlling the Quality of Cell Preparations

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