Recycling of Flammulina filiformis Mushroom Root Waste as Novel Raw Material for the Cultivation of F. filiformis

Shen, Yingyue; Li, Hewen; Li, Qiuyue; Ge, Ningkui; Song, Tingting; Liu, Yalan; Feng, Zhan; Jin, Qunli; Cai, Weiming

doi:10.1007/s12649-023-02278-2

Cited by 1 publication

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have shown that substrates enriched with nitrogen enhance protein concentrations in mushrooms of the Pleurotus genus [27]. The addition of nitrogen source in F. velutipes significantly increased the total protein content, and the total protein content was negatively correlated with the C/N ratio [28]. Additionally, the crude fat content is significantly influenced by the mushrooms' characteristics and the growth conditions, particularly the nitrogen sources in the substrate [29].…”

Section: Nutritional Quality Of Fruiting Bodiesmentioning

confidence: 99%

Effects of Different Carbon and Nitrogen Ratios on Yield, Nutritional Value, and Amino Acid Contents of Flammulina velutipes

Han,

Sun,

Huang

et al. 2024

Life

View full text Add to dashboard Cite

The carbon-to-nitrogen (C/N) ratio in the cultivation medium significantly influences the growth rate, vigor of mycelium, yield of fruiting bodies, and their nutritional composition. Recently, agricultural and forestry wastes have been increasingly used in cultivating Flammulina velutipes. However, systematic research on how these materials affect the nutritional and functional properties of the fruiting bodies is lacking. This study investigated the effects of different C/N ratios on F. velutipes cultivation. We evaluated the agronomic traits, nutritional composition, and flavor compounds of the fruiting bodies. Our findings reveal that an optimal C/N ratio of 27:1 in the composted substrates enhances the total yield of fruiting bodies, with 25.1% soybean straw as the primary raw material. This ratio also significantly increases the levels of crude protein, total amino acids, and essential amino acids in the fruiting bodies (p < 0.05). Fruiting bodies from the high-nitrogen (HN) treatment showed the highest content of umami amino acids and equivalent umami concentration value. Additionally, we employed an untargeted liquid chromatography–mass spectrometry (LC-MS)-based metabolomics approach to analyze the metabolite profiles of fruiting bodies cultivated in high-nitrogen (HN), medium-nitrogen (MN), and low-nitrogen (LN) substrates. We found that the carbon–nitrogen ratio can affect the flavor and quality of fruiting bodies by regulating amino acid biosynthesis and metabolism and other related pathways. Our results suggest that a C/N ratio of 27:1 offers numerous benefits for the cultivation of F. velutipes with comprehensive analyses and has promising application prospects.

show abstract

Section: Nutritional Quality Of Fruiting Bodiesmentioning

confidence: 99%