Recycling immunoaffinity chromatography for multiple analyte analysis in biological samples

Phillips, Terry M.; Krum, Janette M.

doi:10.1016/s0378-4347(97)00675-0

Cited by 40 publications

(31 citation statements)

References 30 publications

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“…The small amount of blood available in the DBSS, combined with the low concentrations of many analytes relevant for monitoring inflammation, however, places high demands on the analytical methods. Multiplexed measurements of cytokines and coagulation factors by recycling immunoaffinity chromatography has been reported, but the method is highly specialized and laborious (24,33 ). We have developed a 25-plex high-capacity method for simultaneous determination of 23 cytokines, TREM-1, and CRP in two 3.2-mm punches of routinely collected neonatal DBSS, based on the commercially available Luminex xMAP technology.…”

Section: Discussionmentioning

confidence: 99%

“…The specificity of the overall assay is therefore intrinsically high, which was also supported by the fact that we observed no cross-reaction within the panel or to other analytes tested. In principle, the method is more specific than recycling immunoaffinity chromatography, in which the specificity relies on reaction between usually polyclonal capture antibodies and fluorochrome-derivatized sample passing through the microcolumns (33 ). Recycling immunoaffinity chromatography would be expected to detect a larger fraction of cytokines interacting with blood proteins, based on the same argument.…”

Section: Discussionmentioning

confidence: 99%

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Simultaneous Measurement of 25 Inflammatory Markers and Neurotrophins in Neonatal Dried Blood Spots by Immunoassay with xMAP Technology

et al. 2005

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Simultaneous Measurement of 25 Inflammatory Markers and Neurotrophins in Neonatal Dried Blood Spots by Immunoassay with xMAP Technology

et al. 2005

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“…The application of immunoaffinity techniques to analyze clinical samples has expanded over the past 5 years and the development of modification to these techniques, that enable multiple analytes to be measured, has advanced it even further (Nelson et al, 1998;Phillips and Krum, 1998 ). The technique can easily be adapted to automated and miniaturized systems therefore making it amenable to clinical and epidemiological studies.…”

Section: Discussionmentioning

confidence: 99%

“…Five immunoaffinity columns were connected to the selector enabling recycling of each sample consecutively through all five columns in a similar fashion to that previously described ( Phillips and Krum, 1998 ). The Scout also permitted elution of each individual column without disturbing the others.…”

Section: Instrumentationmentioning

confidence: 99%

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Assessing environmental exposure in children: immunotoxicology screening

Phillips

2000

J Expo Sci Environ Epidemiol

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Environmental exposure to a number of xenobiotics, including pesticides, can have serious effects on the immune system of children, thus rendering them susceptible to infections or other disease states. To study this problem, a recycling chromatographic system for assessing cytokine profiles in humans has been developed and used for the measurement of immune system function in children with documented exposure to residential pesticides. The system is capable of measuring 30 different analytes in a single sample thus enabling the same time examination of representative markers of immune differentiation and function. In the present study, a cohort of 25 exposed children were examined and shown to exhibit a number of features; all subjects demonstrated some abnormalities in cytokines associated with hematopoiesis. Additionally, elevations in pro -inflammatory cytokines and neuropeptides indicated a state of generalized and neurogenic inflammation. Further analysis indicated that a depression of the cellular arm of the immune system that correlated with clinical indicators of lowered host resistance to infection could also be detected in a subgroup of the exposed subjects. All exposed children demonstrated evidence of hyperstimulation of the humoral immune system as indicated by elevated IL -5 concentrations and clinical allergy. The degree of immune dysregulation in the exposed children was found to be quite marked when compared to similar studies performed on age -matched controls. Journal of Exposure Analysis and Environmental Epidemiology ( 2000 ) 10, 769 ± 775.

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Serum Proteins

Ritchie

Whicher

2000

Encyclopedia of Analytical Chemistry

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Plasma contains a large number of different proteins with varying structural characteristics. The “plasma proteins” have been defined on the basis of plasma concentration and amenability to measurement in certain types of measurement systems. In the past, they were proteins present with concentrations above 0.05 g L −1 , but more recently detection limits have been decreased to about 0.001 g L −1 . However, the group of proteins is not defined on a pathophysiological basis and the definition remains idiosyncratic and incomprehensible to the noncognoscenti. The function of an increasing proportion of them is known and the evidence suggests that they are present either with a specific role in the plasma or that they represent cellular proteins shed into the circulation as a result of degradative processes. Many plasma proteins show characteristic changes in concentration or structure either resulting from genetic dictates that may give rise to disease directly or as a result of changes reflecting pathological or physiological processes. All the transcellular fluids, such as cerebrospinal fluid (CSF), urine, synovial fluid and saliva, contain most or all of the plasma proteins, but there are some additional proteins secreted specifically into these fluids. This chapter reviews the many methods applied to proteins for clinical and research purposes. Also addressed are the pitfalls not often appreciated by the worker presented with a sample to be analyzed in an increasingly impenetrable “machine”.

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Recycling immunoaffinity chromatography for multiple analyte analysis in biological samples

Cited by 40 publications

References 30 publications

Simultaneous Measurement of 25 Inflammatory Markers and Neurotrophins in Neonatal Dried Blood Spots by Immunoassay with xMAP Technology

Simultaneous Measurement of 25 Inflammatory Markers and Neurotrophins in Neonatal Dried Blood Spots by Immunoassay with xMAP Technology

Assessing environmental exposure in children: immunotoxicology screening

Serum Proteins

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