Recursive Least Squares Filtering Algorithms for On-Line Viscoelastic Characterization of Biosamples

Abstract

The mechanical characterization of biological samples is a fundamental issue in biology and related fields, such as tissue and cell mechanics, regenerative medicine and diagnosis of diseases. In this paper, a novel approach for the identification of the stiffness and damping coefficients of biosamples is introduced. According to the proposed method, a MEMS-based microgripper in operational condition is used as a measurement tool. The mechanical model describing the dynamics of the gripper-sample system considers the pseudo-rigid body model for the microgripper, and the Kelvin–Voigt constitutive law of viscoelasticity for the sample. Then, two algorithms based on recursive least square (RLS) methods are implemented for the estimation of the mechanical coefficients, that are the forgetting factor based RLS and the normalised gradient based RLS algorithms. Numerical simulations are performed to verify the effectiveness of the proposed approach. Results confirm the feasibility of the method that enables the ability to perform simultaneously two tasks: sample manipulation and parameters identification.

Selective Laser Sintering PLA/Mg Composite Scaffold with Promoted Degradation and Enhanced Mechanical

“…As a result, the compromised stress transfer mechanism detrimentally impacts the mechanical properties of the composite bone scaffold. This impairment signi cantly compromises the scaffold's ability to effectively transmit and distribute stress, leading to diminished mechanical performance 36,37 . These ndings suggest that, in terms of mechanical properties, the optimal Mg particle content for the composite bone scaffold in this study should be limited to 3%.…”

Design and Validation of a Single-SOI-Wafer 4-DOF Crawling Microgripper

“…MEMS-based microgrippers, equipped with rotary comb-drive actuators, have been presented to characterize the mechanical properties of cells [12,13,14]. Further applications of microgrippers could consists of in vitro simulation of basic surgical operations, by testing and analyzing the microgripper–tissue interactions.…”

Mechanical Response of Four-Bar Linkage Microgrippers with Bidirectional Electrostatic Actuation

“…Another important field of application is biology: manipulation of single cells is an essential step to understand cells behaviors and interactions [9,10]. For example, microgrippers with force sensors where developed for manipulating biological cells [11,12] or to characterize the mechanical properties of biosamples [13,14]. The sensing [15,16] and the control [17,18] of the gripping forces has also been a fundamental issue in developing microgrippers.…”