Recurrent urinary tract infections in healthy and nonpregnant women

Glover, Matthew S.; Moreira, Cristiano G.; Sperandio, Vanessa; Zimmern, Philippe E.

doi:10.1016/j.urols.2013.11.007

Cited by 101 publications

(91 citation statements)

References 89 publications

(102 reference statements)

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“…Previous studies have shown that recurrent UTI can occur as a consequence of reinfection from persistent bacteria or reinitiation of infection from a fecal source (9). Fecal flora can serve as a UPEC reservoir for subsequent recolonization of the bladder (10).…”

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confidence: 99%

Lipopolysaccharide Domains Modulate Urovirulence

et al. 2016

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.1086/599839), we assessed NU14 for Th 2 -associated cytokines. We found NU14 infection stimulated TLR4-dependent bladder interleukin-33 (IL-33) production. Inoculation with rfaG, waaL, wzzE, and wzyE mutants showed decreased IL-33 production. We quantified antigen-specific antibodies after infection and found significantly increased IgE and IgG1 in ⌬waaP mutant-infected mice. Our studies show LPS structural constituents mediate multiple aspects of the UPEC life cycle, including the ability to acutely colonize bladders, form reservoirs, and evoke innate and adaptive immune responses.

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confidence: 99%

Lipopolysaccharide Domains Modulate Urovirulence

et al. 2016

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“…Since T-cell responses can be severely hampered by prolonged and/ or repeated cognate antigenic exposure, 44,45 the activation difference could very well be a consequence of RUTI. In this regard, the presence of intracellular bacterial communities, which are known to be an important pathophysiological factor in RUTI, 46 could contribute to the frequent (re) stimulation of MAIT cells.…”

Section: Discussionmentioning

confidence: 99%

Circulating mucosal‐associated invariant T cells in subjects with recurrent urinary tract infections are functionally impaired

Terpstra

Remmerswaal

Aalderen

et al. 2020

Immunity Inflam & Disease

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Background: Urinary tract infection recurrence is common, particularly in women and immunocompromised patients, such as renal transplant recipients (RTRs). Mucosal-associated invariant T (MAIT) cells play a role in the antibacterial response by recognizing bacterial riboflavin metabolites produced by bacteria such as Escherichia coli. Here, we investigated whether MAIT cells are involved in the pathogenesis of recurrent urinary tract infections (RUTIs).Methods: Using multichannel flow cytometry, we characterized the MAIT cell phenotype and function in blood from immunocompetent adults with (n = 13) and without RUTIs (n = 10) and in RTRs with (n = 9) and without RUTIs (n = 10).Results: There were no differences in the numbers of MAIT cells between the study groups. MAIT cells in patients with RUTI expressed T-bet more often than those in controls. MAIT cells from immunocompetent RUTI participants required more antigen-presenting cells coincubated with E. coli to evoke a similar cytokine and degranulation response than those from controls. This effect was absent in the RTR with RUTI vs RTR control groups, where the overall percentage of MAIT cells that responded to stimulation was already reduced. Conclusion: Circulating MAIT cells in immunocompetent individuals withRUTIs respond to bacterial stimuli with reduced efficacy, which suggests that they are involved in the pathogenesis of RUTIs. K E Y W O R D S MAIT cells, recurrent urinary tract infection, renal transplantation ---

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“…Since T‐cell responses can be severely hampered by prolonged and/or repeated cognate antigenic exposure, 44,45 the activation difference could very well be a consequence of RUTI. In this regard, the presence of intracellular bacterial communities, which are known to be an important pathophysiological factor in RUTI, 46 could contribute to the frequent (re)stimulation of MAIT cells.…”

Section: Discussionmentioning

confidence: 99%

645. Mucosal-Associated Invariant T cells in Renal Tissue From Patients With Recurrent Urinary Tract Infections

Terpstra

Sinnige

Remmerswaal

et al. 2018

Open Forum Infectious Diseases

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BackgroundMucosal associated invariant T (MAIT) cells are innate-like T-cells involved in the antibacterial and fungal response by recognizing riboflavin metabolites produced by these organisms. MAIT cells are present in blood and are highly abundant in the mucosa of the liver, lungs and intestines. In murine models of urinary tract infection (UTI), MAIT cells appear to migrate to the bladder and decrease the bacterial load. It is however unknown whether MAIT cells reside in the human urogenital tract and renal tissue and whether they play a role in the first-line defense against (recurrent) UTI (RUTI).MethodsWe used a fluorescently labelled MR1-tetramer in conjunction with 14-color flowcytometry to identify and characterize MAIT cells in renal allografts after allograft failure caused by RUTI (n = 6) or rejection (n = 6) and in healthy kidney tissue surgically removed because of renal cell carcinoma (adjacent nontumorous tissue) (n = 5).ResultsThe mean percentage of MAIT cells within the lymphogate was higher in the RUTI kidneys (2.24%) compared with the rejection kidneys (0.14%) and the control kidneys (0.11%) (P < 0.05).Characterization of MAIT cells was impossible in some control samples due to MAIT cells counts <25 (predefined cutoff value), therefore the control group was excluded from further statistical analysis.MAIT cells in RUTI kidneys appear to have a less activated profile compared with the rejection kidneys, with a lower expression of Ki67 (P < 0.01). Though the expression of the tissue resident marker CD69/CD103 was higher in 4/6 RUTI kidneys, this difference was not significant.ConclusionMAIT cells are present in renal tissue that is or has been subjected to an immunologic response. MAIT cells in RUTI kidneys display a more quiescent and in some samples more tissue resident phenotype than MAIT cells in rejection kidneys. These findings may suggest that (I) MAIT cells play a role in the first-line defense in the kidney and (II) that after RUTI, MAIT cells remain in renal tissue in a quiescent state. We postulate that this might be favorable in case of a second hit from an uropathogen.Figure 1.Presence and characterization of MAIT cells in renal tissue.Disclosures F. Bemelman, Astellas: We received an unrestricted grant from Astellas to establish a Biobank for patients with renal diseases. The samples described in this abstract are obtained from this Biobank., Grant recipient.

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Recurrent urinary tract infections in healthy and nonpregnant women

Cited by 101 publications

References 89 publications

Lipopolysaccharide Domains Modulate Urovirulence

Lipopolysaccharide Domains Modulate Urovirulence

Circulating mucosal‐associated invariant T cells in subjects with recurrent urinary tract infections are functionally impaired

645. Mucosal-Associated Invariant T cells in Renal Tissue From Patients With Recurrent Urinary Tract Infections

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