Recurrent detection of VIM-1-producing<i>Escherichia coli</i>clone in German pig production

Irrgang, Alexandra; Fischer, Jennie; Grobbel, Mirjam; Schmoger, Silvia; Skladnikiewicz-Ziemer, Tanja; Thomas, Katharina; Hensel, Andreas; Tenhagen, Bernd‐Alois; Käsbohrer, Annemarie

doi:10.1093/jac/dkw479

Cited by 21 publications

(51 citation statements)

References 6 publications

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“…However, newer publications indicate that the trend of finding carbapenemase-producing bacteria in livestock as well as food did not stop. Three recently published manuscripts described the finding of additional VIM-1-positive isolates in Germany: E. coli derived from the colon contents of slaughter pigs (Irrgang et al, 2017 ), as well as two S . Infantis isolated from minced pork meat and a sick piglet (Borowiak et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis

Roschanski¹,

Fischer

Falgenhauer

et al. 2018

Front. Microbiol.

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Carbapenems are last-resort antibiotics used in human medicine. The increased detection of carbapenem-resistant Enterobacteriaceae (CRE) is therefore worrying. In 2011 we reported the first livestock-associated VIM-1-producing Salmonella (S.) enterica serovar Infantis (R3) isolate from dust, sampled in a German chicken fattening farm. Due to this observation we retrospectively investigated more than 536 stored bacterial cultures, isolated from 45 chicken fattening farms during the years 2011 and 2012. After a non-selective overnight incubation, the bacteria were transferred to selective media. Escherichia (E.) coli and Salmonella growing on these media were further investigated, including antibiotic susceptibility testing, carbapenemase gene screening and whole genome sequencing (WGS). In total, four CRE were found in three out of 45 investigated farms: Besides R3, one additional Salmonella (G-336-1a) as well as two E. coli isolates (G-336-2, G-268-2). All but G-268-2 harbored the blaVIM-1 gene. Salmonella isolates R3 and G-336-1 were closely related although derived from two different farms. All three blaVIM-1-encoding isolates possessed identical plasmids and the blaVIM-1- containing transposon showed mobility at least in vitro. In isolate G-268-2, the AmpC beta-lactamase gene blaCMY-2 but no known carbapenemase gene was identified. However, a transfer of the phenotypic resistance was possible. Furthermore, G-268-2 contained the mcr-1 gene, combining phenotypical carbapenem- as well as colistin resistance in one isolate. Carbapenem-resistant Enterobacteriaceae have been found in three out of 45 investigated chicken flocks. This finding is alarming and emphasizes the importance of intervention strategies to contain the environmental spread of resistant bacteria in animals and humans.

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Section: Discussionmentioning

confidence: 99%

Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis

Roschanski¹,

Fischer

Falgenhauer

et al. 2018

Front. Microbiol.

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“…Recent publications have reported the occurrence of carbapenem-nonsusceptible Enterobacteriaceae in wild birds, livestock, and food products and their spread, related to plasmid-mediated carbapenemases ( 11 , 17 – 19 ). As carbapenems are not licensed for use in livestock, it is assumed that the occurrence of carbapenemase-producing bacteria is triggered by coselective pressure, since plasmids carrying bla NDM-1 , like the plasmid chosen for use in this study, commonly harbor multiple but variable resistance determinants ( 16 , 22 ).…”

Section: Discussionmentioning

confidence: 99%

“…The above-mentioned studies and recent reports on VIM-1-producing Escherichia coli and S . Infantis isolates in swine and poultry farms ( 17 , 18 ) showed that the spread and persistence of carbapenemase-producing bacteria in wild birds and livestock are a reality. With recent reports of VIM-1-producing S .…”

Section: Introductionmentioning

confidence: 99%

In Vivo Transfer and Microevolution of Avian Native IncA/C ₂ bla _NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study

Hadžiabdić

Fischer

Malorny

et al. 2018

Antimicrob Agents Chemother

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The emergence and spread of carbapenemase-producing Enterobacteriaceae (CPE) in wildlife and livestock animals pose an important safety concern for public health. With our in vivo broiler chicken infection study, we investigated the transfer and experimental microevolution of the blaNDM-1-carrying IncA/C2 plasmid (pRH-1238) introduced by avian native Salmonella enterica subsp. enterica serovar Corvallis without inducing antibiotic selection pressure. We evaluated the dependency of the time point of inoculation on donor (S. Corvallis [12-SA01738]) and plasmid-free Salmonella recipient [d-tartrate-fermenting (d-Ta+) S. Paratyphi B (13-SA01617), referred to here as S. Paratyphi B (d-Ta+)] excretion by quantifying their excretion dynamics. Using plasmid profiling by S1 nuclease-restricted pulsed-field gel electrophoresis, we gained insight into the variability of the native plasmid content among S. Corvallis reisolates as well as plasmid acquisition in S. Paratyphi B (d-Ta+) and the enterobacterial gut microflora. Whole-genome sequencing enabled us to gain an in-depth insight into the microevolution of plasmid pRH-1238 in S. Corvallis and enterobacterial recipient isolates. Our study revealed that the fecal excretion of avian native carbapenemase-producing S. Corvallis is significantly higher than that of S. Paratyphi (d-Ta+) and is not hampered by S. Paratyphi (d-Ta+). Acquisition of pRH-1238 in other Enterobacteriaceae and several events of plasmid pRH-1238 transfer to different Escherichia coli sequence types and Klebsiella pneumoniae demonstrated an interspecies broad host range. Regardless of the microevolutionary structural deletions in pRH-1238, the single carbapenem resistance marker blaNDM-1 was maintained on pRH-1238 throughout the trial. Furthermore, we showed the importance of the gut E. coli population as a vector of pRH-1238. In a potential scenario of the introduction of NDM-1-producing S. Corvallis into a broiler flock, the pRH-1238 plasmid could persist and spread to a broad host range even in the absence of antibiotic pressure.

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“…The strA/B , catA1 genes together with genes conferring resistance to heavy metals were carried by the same plasmid. Related IncHI2 VIM-1-plasmids were also found in concomitant E. coli strains isolated from German farms [ 35 , 56 , 57 ]. Two additional isolates belonging to the same VIM-1-producing S. Infantis clone were recently reported [ 36 ].…”

Section: Carbapenem Resistance In Non-typhoidal Serovars Of mentioning

confidence: 99%

Resistance to Carbapenems in Non-Typhoidal Salmonella enterica Serovars from Humans, Animals and Food

Fernández

Guerra

Rodicio

2018

Veterinary Sciences

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Non-typhoidal serovars of Salmonella enterica (NTS) are a leading cause of food-borne disease in animals and humans worldwide. Like other zoonotic bacteria, NTS have the potential to act as reservoirs and vehicles for the transmission of antimicrobial drug resistance in different settings. Of particular concern is the resistance to critical “last resort” antimicrobials, such as carbapenems. In contrast to other Enterobacteriaceae (e.g., Klebsiella pneumoniae, Escherichia coli, and Enterobacter, which are major nosocomial pathogens affecting debilitated and immunocompromised patients), carbapenem resistance is still very rare in NTS. Nevertheless, it has already been detected in isolates recovered from humans, companion animals, livestock, wild animals, and food. Five carbapenemases with major clinical importance—namely KPC (Klebsiella pneumoniae carbapenemase) (class A), IMP (imipenemase), NDM (New Delhi metallo-β-lactamase), VIM (Verona integron-encoded metallo-β-lactamase) (class B), and OXA-48 (oxacillinase, class D)—have been reported in NTS. Carbapenem resistance due to the production of extended spectrum- or AmpC β-lactamases combined with porin loss has also been detected in NTS. Horizontal gene transfer of carbapenemase-encoding genes (which are frequently located on self-transferable plasmids), together with co- and cross-selective adaptations, could have been involved in the development of carbapenem resistance by NTS. Once acquired by a zoonotic bacterium, resistance can be transmitted from humans to animals and from animals to humans through the food chain. Continuous surveillance of resistance to these “last resort” antibiotics is required to establish possible links between reservoirs and to limit the bidirectional transfer of the encoding genes between S. enterica and other commensal or pathogenic bacteria.

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Recurrent detection of VIM-1-producingEscherichia coliclone in German pig production

Cited by 21 publications

References 6 publications

Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis

Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis

In Vivo Transfer and Microevolution of Avian Native IncA/C ₂ bla _NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study

Resistance to Carbapenems in Non-Typhoidal Salmonella enterica Serovars from Humans, Animals and Food

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Recurrent detection of VIM-1-producingEscherichia coliclone in German pig production

Cited by 21 publications

References 6 publications

Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis

Retrospective Analysis of Bacterial Cultures Sampled in German Chicken-Fattening Farms During the Years 2011–2012 Revealed Additional VIM-1 Carbapenemase-Producing Escherichia coli and a Serologically Rough Salmonella enterica Serovar Infantis

In Vivo Transfer and Microevolution of Avian Native IncA/C 2 bla NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study

Resistance to Carbapenems in Non-Typhoidal Salmonella enterica Serovars from Humans, Animals and Food

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In Vivo Transfer and Microevolution of Avian Native IncA/C ₂ bla _NDM-1 -Carrying Plasmid pRH-1238 during a Broiler Chicken Infection Study