Recrystallization of Ice Crystals in Trehalose Solution at Isothermal Condition

Quantitative Analysis of Glassy State Relaxation and Ostwald Ripening during Annealing Using Freeze-Drying Microscopy

“…The differences in the coarsening rates can be explained with the water mobility in each solution. Klinmalai et al [27] assumed that water molecules interact in two different ways with saccharides -low interaction for so-called free water and stronger interaction for so-called associating water. The ratio of free water to associating water will thus have an impact on the net water mobility of the solution.…”

Small-molecule fulvic acid with strong hydration ability for non-vitreous cellular cryopreservation

“…One effective strategy is mimicking the adsorption inhibition mechanism of AFP through designing AFP-similar repeated ice-binding/amphiphilic structures. Besides, some works demonstrated the importance of hydration, and proposed the linear correlation between the ice recrystallization activity and the hydration ability of small molecular carbohydrates ( Czechura et al., 2008 ; Klinmalai et al., 2017 ; Tam et al., 2008 ), implying an alternative, simple strategy of improving hydration for designing ice growth/recrystallization inhibition materials. Although many ice-control materials were reported and some of them indeed displayed attractive ice growth/recrystallization inhibition abilities, only a part of them showed potential application in cryopreservation area, and very few especially small molecules could achieve non-vitreous cell cryopreservation without needing any permeable cryoprotectants such as glycerol and dimethyl sulfoxide.…”

Transformation process of ice crystallized from a glassy dilute trehalose aqueous solution

“…The effects of trehalose on liquid water and crystalline ice are studied in broad scientific fields such as solution chemistry, chemical physics, biology, medical science and food engineering. [3][4][5][6][7][8][9][10][11][12] However, the interaction between trehalose and water is not yet fully understood and the authenticity of the peculiarity of trehalose is debated actively.…”

“…There are several reports on the effect of trehalose on ice during the cooling process of a trehalose aqueous solution. 10,12,13 However, there are few studies on the effect of trehalose on glassy water and crystalline ice after crystallization of the glassy water.…”