Recruitment of the 40S ribosomal subunit by the West Nile virus 3′ UTR promotes the cross-talk between the viral genomic ends for translation regulation

Ramos-Lorente, Sara Esther; Berzal-Herranz, Beatriz; Romero-López, Cristina; Berzal-Herranz, Alfredo

doi:10.1016/j.virusres.2024.199340

Cited by 2 publications

(1 citation statement)

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“…The 5 ′ UTR is about 100 nucleotides long and contains elements with significant roles in replication and translation [8,9]. The 3 ′ UTR is relatively larger, reaching approximately 630 nucleotides [10]. It is divided into three highly structured domains.…”

Section: Introductionmentioning

confidence: 99%

West Nile Virus Subgenomic RNAs Modulate Gene Expression in a Neuronal Cell Line

Bampali,

Kouvela,

Kesesidis

et al. 2024

Viruses

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Subgenomic flaviviral RNAs (sfRNAs) are small non-coding products of the incomplete degradation of viral genomic RNA. They accumulate during flaviviral infection and have been associated with many functional roles inside the host cell. Studies so far have demonstrated that sfRNA plays a crucial role in determining West Nile virus (WNV) pathogenicity. However, its modulatory role on neuronal homeostasis has not been studied in depth. In this study, we investigated the mechanism of sfRNA biosynthesis and its importance for WNV replication in neuronal cells. We found that sfRNA1 is functionally redundant for both replication and translation of WNV. However, the concurrent absence of sfRNA1 and sfRNA2 species is detrimental for the survival of the virus. Differential expression analysis on RNA-seq data from WT and ΔsfRNA replicon cell lines revealed transcriptional changes induced by sfRNA and identified a number of putative targets. Overall, it was shown that sfRNA contributes to the viral evasion by suppressing the interferon-mediated antiviral response. An additional differential expression analysis among replicon and control Neuro2A cells also clarified the transcriptional changes that support WNV replication in neuronal cells. Increased levels of translation and oxidative phosphorylation, post-translational modification processes, and activated DNA repair pathways were observed in replicon cell lines, while developmental processes such as axonal growth were deficient.

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Section: Introductionmentioning

confidence: 99%