Recruitment of NIMA kinase shows that maturation of the<i>S. pombe</i>spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity

Grallert, Ágnes; Krapp, Andrea; Bagley, Steven; Hagan, Iain

doi:10.1101/gad.296204

Cited by 97 publications

(137 citation statements)

References 71 publications

(79 reference statements)

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“…Therefore, we investigated whether Ppa2p, Ypa1p, and Ypa2p also affect the asymmetric segregation of Cdc7p to the new SPB during anaphase. In wild-type cells, Cdc7p is associated with both SPBs in early mitosis in all cells, becoming asymmetric in late anaphase as it remains associated only with the new SPB (Sohrmann et al 1998;Grallert et al 2004). In late anaphase Cdc7p-GFP was associated with only one SPB in the great majority of cells (59 of 60 cells; Figure 5A), as described previously (Sohrmann et al 1998;Grallert et al 2004).…”

Section: Ypa2-7 Ndmentioning

confidence: 69%

“…In wild-type cells, Cdc7p is associated with both SPBs in early mitosis in all cells, becoming asymmetric in late anaphase as it remains associated only with the new SPB (Sohrmann et al 1998;Grallert et al 2004). In late anaphase Cdc7p-GFP was associated with only one SPB in the great majority of cells (59 of 60 cells; Figure 5A), as described previously (Sohrmann et al 1998;Grallert et al 2004). Examination of late anaphase ypa2-D cells revealed an increase in the number of cells that retained Cdc7p-GFP on both SPBs (8 of 60, compared to 1 of 60 in wild type; Figure 5A).…”

Section: Ypa2-7 Ndmentioning

confidence: 69%

“…Laser ablation of SPBs suggests that at least one SPB must be intact during anaphase B for cytokinesis to occur (Magidson et al 2006). Duplication of the S. pombe SPB appears to be conservative (Ding et al 1997;Grallert et al 2004), generating distinguishable old and new SPBs (Grallert et al 2004). Some of the SIN proteins (Cdc7p, Cdc16p, Byr4p, Sid1p, and Cdc14p) show asymmetric localization on one of the two SPBs during mitosis (Sohrmann et al 1998;Cerutti and Simanis 1999;Guertin et al 2000;Li et al 2000), which is thought to be important for regulating SIN activity (Garcia-Cortes and McCollum 2009).…”

Section: Sin Signaling Requires Association Of Sin Proteins With the mentioning

confidence: 99%

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Characterization of ypa1 and ypa2, the Schizosaccharomyces pombe Orthologs of the Peptidyl Proyl Isomerases That Activate PP2A, Reveals a Role for Ypa2p in the Regulation of Cytokinesis

Goyal

2012

Genetics

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The Schizosaccharomyces pombe septation initiation network (SIN) regulates cytokinesis. Cdc7p is the first kinase in the core SIN; we have screened genetically for SIN regulators by isolating cold-sensitive suppressors of cdc7-24. Our screen yielded a mutant in SPAC1782.05, one of the two fission yeast orthologs of mammalian phosphotyrosyl phosphatase activator. We have characterized this gene and its ortholog SPAC4F10.04, which we have named ypa2 and ypa1, respectively. We find that Ypa2p is the major form of protein phosphatase type 2A activator in S. pombe. A double ypa1-D ypa2-D null mutant is inviable, indicating that the two gene products have at least one essential overlapping function. Individually, the ypa1 and ypa2 genes are essential for survival only at low temperatures. The ypa2-D mutant divides at a reduced cell size and displays aberrant cell morphology and cytokinesis. Genetic analysis implicates Ypa2p as an inhibitor of the septation initiation network. We also isolated a cold-sensitive allele of ppa2, the major protein phosphatase type 2A catalytic subunit, implicating this enzyme as a regulator of the septation initiation network. SCHIZOSACCHAROMYCES pombe cells take the form of a cylinder capped by hemispherical ends. During interphase, cells grow mainly at their tips. Cell elongation ceases after mitotic commitment, and the cytoskeleton is reorganized in preparation for nuclear and cell division (reviewed by McCully and Robinow 1971;Mitchison and Nurse 1985;Marks et al. 1986;Hagan and Hyams 1988;Hagan 1998). The cell divides by fission after forming a septum in the middle of the cell. The site of division is defined at the onset of mitosis (Daga and Chang 2005), by the formation of an actomyosin contractile ring (CAR), whose assembly continues throughout mitosis (Wu et al. 2003;Pollard 2008;Pollard and Wu 2010). At the end of anaphase, the CAR is thought to guide synthesis of the multilayered division septum (reviewed by (Ishiguro 1998;Sipiczki 2007). Septation Initiation NetworkThe S. pombe septation initiation network (SIN) is a network of protein kinases that is essential for cytokinesis in the mitotic cycle; reviewed by (Gould and Simanis 1997;Simanis 2003;Wolfe and Gould 2005;Goyal et al. 2011). It is tightly regulated through the mitotic cell cycle to assure proper coordination of mitosis and cytokinesis, and during meiosis, where it is essential for spore formation (Krapp et al. 2006). Signaling requires the activity of three protein kinases, each of which has a regulatory subunit (kinase regulator); Cdc7p-Spg1p (Fankhauser and Simanis 1994;Schmidt et al. 1997;Mehta and Gould 2006), Sid1p-Cdc14p (Fankhauser and Simanis 1993;Guertin et al. 2000;Guertin and McCollum 2001), and Sid2p-Mob1p (Sparks et al. 1999;Hou et al. 2000;Salimova et al. 2000). SIN signaling is modulated by the nucleotide status of the GTPase Spg1p (Schmidt et al. 1997;Sohrmann et al. 1998), which is determined by the balance of spontaneous nucleotide exchange, a putative GEF, Etd1p Garcia-Cortes and McCollu...

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Section: Ypa2-7 Ndmentioning

confidence: 69%

Section: Ypa2-7 Ndmentioning

confidence: 69%

Section: Sin Signaling Requires Association Of Sin Proteins With the mentioning

confidence: 99%

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Characterization of ypa1 and ypa2, the Schizosaccharomyces pombe Orthologs of the Peptidyl Proyl Isomerases That Activate PP2A, Reveals a Role for Ypa2p in the Regulation of Cytokinesis

Goyal

2012

Genetics

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“…Although the asymmetric competence for signaling of two otherwise indistinguishable SPBs arises primarily from extrinsic, spatial context cues to drive mitotic exit of budding yeast, it is primarily the age of the SPB and not external cues that determines which of the two anaphase SPBs will host the active version of the fission yeast equivalent of the MEN (Simanis 2003;Grallert et al 2004). Thus, even in the simple single-celled yeasts, different strategies can turn one of the two spindle poles into a unique signaling platform from which to determine cell fate.…”

Section: Intrinsic Controls Set By Spb Maturation Establishes One Spbmentioning

confidence: 99%

The Centrosome and Its Duplication Cycle

Hagan

Glover

2015

Cold Spring Harb Perspect Biol

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“…In anaphase B, Cdc7p (Grallert et al, 2004;Sohrmann et al, 1998) and Sid1p (Guertin et al, 2000) associate with the new SPB (nSPB), whereas Byr4p and Cdc16p associate with the old SPB (oSPB) (Cerutti and Simanis, 1999;Li et al, 2000). Mutants that compromise asymmetric distribution of SIN proteins on the SPBs during mitosis deregulate septation (García-Cortés and McCollum, 2009;Singh et al, 2011;Sohrmann et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Analysis ofS. pombeSIN protein SPB-association reveals two genetically separable states of the SIN

Wachowicz

Chasapi

Krapp

et al. 2014

Journal of Cell Science

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The Schizosaccharomyces pombe septation initiation network (SIN) regulates cytokinesis, and asymmetric association of SIN proteins with the mitotic spindle pole bodies (SPBs) is important for its regulation. Here, we have used semi-automated image analysis to study SIN proteins in large numbers of wild-type and mutant cells. Our principal conclusions are: first, that the association of Cdc7p with the SPBs in early mitosis is frequently asymmetric, with a bias in favour of the new SPB; second, that the early association of Cdc7p-GFP to the SPB depends on Plo1p but not Spg1p, and is unaffected by mutations that influence its asymmetry in anaphase; third, that Cdc7p asymmetry in anaphase B is delayed by Pom1p and by activation of the spindle assembly checkpoint, and is promoted by Rad24p; and fourth, that the length of the spindle, expressed as a fraction of the length of the cell, at which Cdc7p becomes asymmetric is similar in cells dividing at different sizes. These data reveal that multiple regulatory mechanisms control the SIN in mitosis and lead us to propose a two-state model to describe the SIN.

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Recruitment of NIMA kinase shows that maturation of theS. pombespindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity

Abstract: Mitotic exit in

Cited by 97 publications

References 71 publications

Characterization of ypa1 and ypa2, the Schizosaccharomyces pombe Orthologs of the Peptidyl Proyl Isomerases That Activate PP2A, Reveals a Role for Ypa2p in the Regulation of Cytokinesis

Characterization of ypa1 and ypa2, the Schizosaccharomyces pombe Orthologs of the Peptidyl Proyl Isomerases That Activate PP2A, Reveals a Role for Ypa2p in the Regulation of Cytokinesis

The Centrosome and Its Duplication Cycle

Analysis ofS. pombeSIN protein SPB-association reveals two genetically separable states of the SIN

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