Recruitment of neutrophils during IgE-dependent cutaneous late phase reactions in the mouse is mast cell-dependent. Partial inhibition of the reaction with antiserum against tumor necrosis factor-alpha.

Abstract

Introduction

Modulation by IL-10 of antigen-induced allergic responses in mice

“…2B). These results extend those from previous studies showing the participation of TNF-α in leukocyte infiltration associated with IgE-dependent cutaneous inflammation in sensitized mice (Wershil et al 1991). Together, these results suggest that the inhibitory effect of IL-10 probably involved downregulation of TNF-α generation in the BAL fluid, since treatment of sensitized mice with a specific anti-TNF-α antiserum drastically reduced airway eosinophilia.…”

NF-κB and TNF-α: A Positive Autocrine Loop in Human Lung Mast Cells?

“…Furthermore, the sustained release of preformed and newly synthesized TNF-␣ following appropriate stimulation represents a mechanism by which mast cell-derived TNF-␣ can exert its actions on leukocyte migration and activation and the initiation of late-phase allergic inflammation. With the use of KO mice and adoptive transfer experiments, mast cell-derived TNF-␣ has been shown to contribute significantly to leukocyte infiltration (36). Also, unlike macrophages and lymphocytes, which contain little or no preformed TNF-␣, this cytokine is stored in human mast cells in many tissue sites (5,6,37).…”

Immunomodulatory mast cells: negative, as well as positive, regulators of immunity

“…Some of the positive immunomodulatory functions of mast cells that have been proposed based on in vitro studies have been confirmed in vivo using mast cell knock-in mice 1,54,[57][58][59][60][61][62][63][64][65][66][67][68][69][70] or in mice lacking specific mast-cell-associated proteases [26][27][28][32][33][34] or lacking specific protease enzymatic activity 11 (Table 1 ). In many of these studies, the end points assessed included the recruitment of particular immune cells, such as granulocytes 28,32,33,[57][58][59][60][61]63,64,[67][68][69][70] , DCs 62,64,65,71 or various subpopulations of lymphocytes 64,67,70,72 .…”

“…In many of these studies, the end points assessed included the recruitment of particular immune cells, such as granulocytes 28,32,33,[57][58][59][60][61]63,64,[67][68][69][70] , DCs 62,64,65,71 or various subpopulations of lymphocytes 64,67,70,72 . Many of these studies also demonstrated that the lack of mast cells 54,[57][58][59][60][61][62][63][64]67,69,70 or a specific mast-cell product 28,32,33,54,60,63,67,68,70 also reduced the pathology associated with the immune response or impaired its effectiveness in promoting host resistance to infection.…”