Recruitment of multiple cell lines by collagen-synthetic copolymer matrices in corneal regeneration

Li, F.; Griffith, May; Li, Z.; Tanodekaew, S; Sheardown, Heather; Hakim, M. O.; Carlsson, D. J.

doi:10.1016/j.biomaterials.2004.07.063

Cited by 88 publications

(79 citation statements)

References 43 publications

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“…The maximum achievable YIGSR concentration of 3.1 x 10-2 mg/ml collagen is significantly greater than that obtained previously using the PNiPAAmbased crosslinking agent at 1.6x10-6 mg/mg of collagen. 44 More recently, Liu et al showed that biologically interactive corneal substitutes could also be fabricated from interpenetrating polymeric networks of collagen and a synthetic phosphorylcholine (lipid). 45 In this case, one network comprised of collagen (either porcine or recombinant human) crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS).…”

Section: B Hybrid Scaffolds and Nanocomposites For Promoting Regenermentioning

confidence: 99%

Regenerative Approaches as Alternatives to Donor Allografting for Restoration of Corneal Function

et al. 2012

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A range of alternatives to human donor tissue for corneal transplantation are being developed to address the shortfall of good quality tissues as well as the clinical conditions for which allografting is contraindicated. Classical keratoprostheses, commonly referred to as artificial corneas, are being used clinically to replace minimal corneal function. However, they are used only as last resorts, as they are associated with significant complications, such as extrusion/rejection, glaucoma, and retinal detachment. The past few years have seen significant developments in technologies designed to replace part or the full thickness of damaged or diseased corneas with materials that encourage regeneration to different extents. This review describes selected examples of these corneal substitutes, which range from cell-based regenerative strategies to keratoprostheses with regenerative capabilities via tissue-engineered scaffolds pre-seeded with stem cells. It is unlikely that one corneal substitute will be best for all indications, but taken together, the various approaches may soon be able to supplement the supply of human donor corneas for transplantation or allow restoration of diseased or damaged corneas that cannot be treated by currently available techniques.

Funding Agencies|Swedish Research Council, County Council of Ostergotland, Sweden||Canadian Stem Cell Network||NSERC Canada|SAF2011-22500|Spanish Ministerio de Economia y Competitividad||

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Section: B Hybrid Scaffolds and Nanocomposites For Promoting Regenermentioning

confidence: 99%

Regenerative Approaches as Alternatives to Donor Allografting for Restoration of Corneal Function

et al. 2012

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Funding Agencies|Swedish Research Council, County Council of Ostergotland, Sweden||Canadian Stem Cell Network||NSERC Canada|SAF2011-22500|Spanish Ministerio de Economia y Competitividad||

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“…Innervation also stimulated twofold epithelial and keratocyte proliferation, better epithelial stratification, the establishment of a protective mucin layer by day 10, and a greater resistance to toxic agents, when compared with noninnervated constructs (52). In a further effort to improve corneal innervation and epithelial reconstruction, the group developed a copolymer [poly(N-isopropylacrylamidecoacrylic acid-coacryloxysuccinimide), PNiPAAm-coAAccoASI, designated TERP and an analog containing the YIGSR cell adhesion motif, designated TERP-5 (53,54). These materials were reacted with collagen to form crosslinked gels, which were then cast on top of the standard collagen-CS matrices; this construct was then surrounded by a collagen-CS ring, which was seeded with DRGs.…”

Section: Advances In Tissue-engineered Corneal Equivalentsmentioning

confidence: 99%

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

et al. 2008

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ABSTRACT:The field of corneal tissue engineering has made many strides in recent years. The challenges of engineering a biocompatible, mechanically stable, and optically transparent tissue are significant. To overcome these challenges, researchers have adopted two basic approaches: cell-based strategies for manipulating cells to create their own extracellular matrix, and scaffold-based strategies for providing strong and transparent matrices upon which to grow cells. Both strategies have met with some degree of success. In addition, recent advances have been made in innervating a tissueengineered construct. Future work will need to focus on further improving mechanical stability of engineered constructs as well as improving the host response to implantation.

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“…[1][2][3] Many thermosensitive polymer solutions have a lower critical solution temperature (LCST), a temperature at which these polymers in aqueous solution experience a phase change from a soluble state to an insoluble state. Poly(NIPAAm) exhibits a LCST at 32°C.…”

Section: Introductionmentioning

confidence: 99%

New Hydrolysis-Dependent Thermosensitive Polymer for an Injectable Degradable System

2007

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Novel, bioerodible, thermosensitive poly(NIPAAm-co-dimethyl-γ-butyrolactone), with hydrolysisdependent thermosensitivity, were synthesized by radical polymerization with varying dimethyl-γ-butyrolactone content and the properties of the copolymers were characterized using Differential Scanning Calorimetry (DSC), High Performance Liquid Chromatography (HPLC) in conjunction with Static Light Scattering, Fourier Transformed Infrared Spectroscopy (FTIR), Nuclear Magnetic Resonance (NMR) and acid titration. The lower critical solution temperature (LCST) of the copolymers decreased with increasing dimethyl-γ-butyrolactone content, but then increased after ring-opening hydrolysis of the dimethyl-γ-butyrolactone side group. FTIR and NMR spectra showed the copolymerization of these two monomers and the hydrolysis-dependent ring-opening of the dimethyl-γ-butyrolactone side group. It was also found that there are no low-molecular-weight byproducts but rather dissolution of the polymer chains at 37°C during the time frame of application. Models of the kinetics suggest that the hydrolysis reaction is self-catalytic due to an increase in hydrophilicity, and thus accessible water concentration, caused by ring-opening of the dimethyl-γ-butyrolactone.

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Recruitment of multiple cell lines by collagen-synthetic copolymer matrices in corneal regeneration

Cited by 88 publications

References 43 publications

Regenerative Approaches as Alternatives to Donor Allografting for Restoration of Corneal Function

Regenerative Approaches as Alternatives to Donor Allografting for Restoration of Corneal Function

The Development of a Tissue-Engineered Cornea: Biomaterials and Culture Methods

New Hydrolysis-Dependent Thermosensitive Polymer for an Injectable Degradable System

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