Recruitment of intestinal CD45RA+ and CD45RC+ cells induced by a candidate oral vaccine against porcine post-weaning colibacillosis

Božić, Frane; Lacković, Gordana; Stokes, Christopher; Valpotić, Ivica

doi:10.1016/s0165-2427(02)00033-8

Cited by 20 publications

(18 citation statements)

References 27 publications

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“…Such immunostimulatory effects of the copolymers on Th1 and Th2 responses following the facilitation of uptake of exogenous antigens by APC have been reported [13]. The diversity of the immune cell subsets that could be stimulated by POE-POP in the postweanling pig model system, it suggests that the copolymers when applied as the adjuvant with specific vaccinal immunogen (non-ETEC strain) or alone as an IRM (applied in the current study), may affect either the immunologically commited (T, B and NK-cells) or naïve (CD45RA + ) lymphoid cells [29]. Consistent with a former finding of selectively activated CD1 + and CD21 + gut-residing B cells is our previous finding of stimulated proliferation of IgA + plasma cells and sIgA antibodies by the immunization with POE-POP adjuvanted non-ETEC vaccine candidate strain [20,32].…”

Section: Discussionmentioning

confidence: 88%

“…A single cell suspensions (100 μL) were prepared in triplicates (comprising 10,000 cells each) and incubated with mAbs (50 μL) (Table 1) and processed as described previously [29]. The fluorescence of the mAb-labelled porcine lymphoid cells was quantified using a Coulter EPICS-XL flow cytometer (Beckman Coulter, Miami, FL, USA) as detailed earlier [30].…”

Section: Methodsmentioning

confidence: 99%

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Effect of polyoxyethylene and polyoxypropylene nonionic block copolymers on performance and recruitment of immune cell subsets in weaned pigs

et al. 2013

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BackgroundBecause European-wide directives are restricting the non-clinical use of antibiotics as in-feed growth promotors in swine production, there is an intensive search for alternative strategies for control and prevention of losses among young pigs. With the growing knowledge of the porcine immune system and its endogenous modulation, it has been clearly established that exogenous immunomodulation using adjuvants and immune response modifiers (IRMs) represents an important prophylactic/therapeutic approach in the prevention/treatment of both stress- and microbial-induced disorders that accompaning weaning. However, it is essential to select a fully evaluated agent which may act either as a nonspecific IRM or synergistically as an adjuvant with vaccines. The synthetic macromolecules with a long history as adjuvant and IRM are nonionic block copolymers which consist of polyoxyethylene (POE) and polyoxypropylene (POP) molecules.MethodsThe aim of this work was to evaluate the effectiveness of POE-POP given as a single peroral dose on productivity parameters such as body weight gain, feed intake and feed conversion ratio, and systemic and intestinal immune parameters by assessing the proportions of CD45+ lymphoid cells, CD4+ and CD8+ T cells, and CD21+ B cells in the peripheral blood as well as the number of CD45RA+ naive lymphoid cells residing in the ileal mucosa in weaned pigs during a follow-up study 5 weeks after the treatment.ResultsPigs treated with POE-POP had better feed intake (+ 14.57%), higher average body mass at the end of the experiment (20.91 kg vs. 17.61 kg), and higher body weight gain in relation to Day 0 (191.63% vs. 144.58%) as well as in relation to nontreated pigs (+ 18.74%), with a lower feed conversion ratio (− 30.26%) in comparison to the control pigs. A much lower diarrhea severity score (5 vs. 54) was recorded in pigs treated with POE-POP (− 90.74%) than in the control pigs. A higher average diarrhea severity (ADS) was recorded in the control pigs (1.54 vs. 0.14), whereas the treatmant group had much a lower ADS ratio (− 90.91%) after 35 days of the experiment. The pigs that were treated with POE-POP had an increased proportion of CD45+, CD4+ and CD8+ cells at Day 21 (at p < 0.05, p < 0.05 or p < 0.01, respectively), Day 28 (at p < 0.01, respectively) and Day 35 (at p < 0.01, p < 0.05 or p < 0.01, respectively) as well as of CD21+ cells at Day 28 (p < 0.05) and Day 35 of the experiment (p < 0.01). Also, these pigs had more numerous CD45RA+ cells in interfollicular (p < 0.05) and follicular areas (p < 0.01) of the ileal Peyer’s patches than did control pigs.ConclusionThis property of POE-POP to induce recruitment of circulating and intestinal immune cell subsets in weaned pigs may allow the use of IRM-active block copolymers as adjuvants for vaccines, particularly those orally delivered and targeted to the gut-associated lymphoid tissues that are well known to promote rather tolerogenic than protective immune responses.

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Section: Discussionmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Effect of polyoxyethylene and polyoxypropylene nonionic block copolymers on performance and recruitment of immune cell subsets in weaned pigs

et al. 2013

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“…One million passage 3 ASCs were suspended in 100 μL PBS containing 10 μg/ml fluorescein isothiocyanate-conjugated primary antibodies specific to mesenchymal stromal cells (MSCs) (CD29, CD44, CD90, and CD105) and hematopoietic cells (CD31 and CD45) (n = 3). The following expression markers reactive with the porcine antigen isoforms were used: Alexa Fluor 647 Mouse Anti-Pig CD29 (BD Bioscience, New Jersey, USA), Anti-CD44 antibody [IM7] (Abcam plc, Cambridge, UK), APC Mouse Anti-Human CD90 (BD-Biosciences), Anti-CD105 antibody [MEM-229] (Abcam plc), PE Mouse Anti-Rat CD31 (BD Biosciences), and Monoclonal Antibody to CD45/LCA (CD45R)-PE (Acris Antibodies, Inc. CA, USA) [24] , [25] , [26] , [27] , [28] . Cell fluorescence was evaluated with a Gallios flow cytometer (Beckman Coulter, Tokyo, Japan) and the data were analyzed using Karuza for Gallios software (Beckman Coulter).…”

Section: Methodsmentioning

confidence: 99%

Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis

Maruya¹,

Kanai²,

Kobayashi³

et al. 2017

Regenerative Therapy

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ObjectiveAdipose-derived stem cells (ASCs) are capable of multiple differentiation pathways, imparting immunomodulatory effects, and secreting factors that are important for wound healing. These characteristics can be exploited to decrease the incidence of anastomotic leakage.MethodsIn order to delay local wound healing at the anastomotic site, we induced ischemia in a portion of porcine small intestine by ligating vessels. Then, we injected mitomycin C into the serosa of the small intestine above the ligated vessels. Anastomotic sites were created by 2 cm incisions made in the opposite mesenteric area. ASCs were isolated from the porcine subcutaneous fat tissues and expanded under culture conditions. ASCs were trypsinized and seeded on temperature-responsive dishes and cultured to form confluent sheets. Three ASC sheets were transplanted onto the serous membrane after suturing. The extent of anastomotic wound healing was evaluated by bursting pressure, hydroxyproline content, and mRNA expression of collagen-1 alpha1 and collagen-3 alpha1.ResultsWe found that transplantation of ASC sheets increased anastomotic site bursting pressure. Additionally, transplantation of ASC sheets increased the hydroxyproline content of the anastomoses. Furthermore, transplantation of ASC sheets increased mRNA expression of collagen-1 alpha1 and collagen-3 alpha1.ConclusionsOur findings showed that transplantation of autologous ASC sheets enhanced collagen synthesis and anastomotic strength. Further studies are necessary to identify substances that, in combination with ASC sheets, might enhance collagen synthesis and healing in sites of anastomosis.

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“…Blood samples (1 ml) from 7 pigs in each group were taken weekly from vena cava cranialis into glass tubes (Beckton Dickinson, Plymouth, UK) with ethylenediaminetetraacetic acid (EDTA) (Sigma, Germany) as anticoagulant for FCM analysis as described previously (Valpotić et al 1994;Božić et al 2002).…”

Section: Bacteriologymentioning

confidence: 99%

Effect of mannan oligosaccharide supplementation on blood and intestinal immune cells, bacteria numbers and performance in weaned pigs

et al. 2016

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In animal production, alternative strategies to the use of antibiotic growth promoters have stimulated research of dietary supplements to increase resistance, reduce post-weaning gut disorders and growth checks in pigs. This study was performed to determine the influence of dietary mannan oligosaccharide as a potential alternative to antibiotic growth promoters in weaned pigs on: (1) proliferation of circulating and intestinal immune cell subsets, (2) incidence and severity of diarrhoea, and (3) performance during 35 days of trial. Forty-six pigs from a commercial farm were divided into two groups comprising 23 pigs each and treated at 4 weeks of age as follows: controls received standard weaner diet, whereas diet for principals was supplemented with 0.2% of mannan oligosaccharide. The pigs were monitored/sampled on daily, weekly or monthly basis for feed efficiency, growth rate, diarrhoea severity scores, blood samples, rectal swabs and intesinal samples for bacterial isolation/counts and immunohistology/ histomorphometry analyses. The principals were heavier (P < 0.05) at 28 days and grew faster between days 7 and 28 compared to controls. Total diarrhoea score recorded in these pigs was decreased by 20.37%. Total bacterial load in jejunum was decreased in these pigs (23 × 10 7 vs. 19 × 10 8 colony forming units/ml) at day 35. Between days 28 and 35 or days 21 and 28, the principals increased in proportions of circulating CD45 + , CD4 + , CD21 + , CD8 + cells (P < 0.01), respectively. These pigs had increased (P < 0.05) the number of CD45RA + cells in interfollicular and follicular areas of ileal Payer's patches at day 35. We concluded that dietary modulation with mannan oligosaccharide resulted in stimulation of immune responses and reduced number of gut microbiota, but not necessarily promoting the growth of pigs.

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Recruitment of intestinal CD45RA+ and CD45RC+ cells induced by a candidate oral vaccine against porcine post-weaning colibacillosis

Cited by 20 publications

References 27 publications

Effect of polyoxyethylene and polyoxypropylene nonionic block copolymers on performance and recruitment of immune cell subsets in weaned pigs

Effect of polyoxyethylene and polyoxypropylene nonionic block copolymers on performance and recruitment of immune cell subsets in weaned pigs

Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis

Effect of mannan oligosaccharide supplementation on blood and intestinal immune cells, bacteria numbers and performance in weaned pigs

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