Recruitment and selection of ovarian follicles for determination of ovulation rate in the pig

Knox, Robert V.

doi:10.1016/j.domaniend.2005.02.025

Cited by 92 publications

(111 citation statements)

References 45 publications

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“…According to Brüssow and Wähner [35], PMSG is the only agent that can stimulate sufficient ovulatory follicles to produce large viable litters. However, do Lago et al [36] and Martinat-Botté et al [3] found that PMSG treatment increased the ovulation rate, but also had a negative influence on embryonic viability, probably because it increased follicular heterogeneity in the preovulatory pool and caused the asynchronous development of embryos [37,38].…”

Section: Paritymentioning

confidence: 99%

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Effect of a GnRH analogue (Maprelin) on the reproductive performance of gilts and sows

Jong

Kauffold²,

Engl³

et al. 2013

Theriogenology

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a b s t r a c tThe ability of peforelin (l-GnRH-III) to stimulate follicular growth, FSH release, and estrus in gilts after altrenogest treatment and in sows after weaning was investigated. In three farrow-to-wean herds, with at least 600 sows and average production performance, 216 gilts, 335 primiparous, and 1299 pluriparous sows were randomly allocated to three treatments: peforelin (M group: Maprelin), eCG (F group: Folligon), and physiological saline solution (C group). Animals were treated 48 hours after their last altrenogest treatment (gilts) or 24 hours after weaning (sows). The weaning-to-estrus interval, estrus duration, estrus rate (ER), pregnancy rate, and total born (TB), live born, and stillborn (SB) numbers were recorded and compared between treatments for the different parity groups (gilts and primiparous and pluriparous sows). Follicle sizes were measured in representative animals from each group on the occasion of their last altrenogest treatment or at weaning, and also on the occasions of their first (FS1) and second (FS2) attempted inseminations. Blood samples were taken to determine FSH concentrations at weaning and 2 hours after injection, and progesterone concentrations 10 days after the first insemination attempt. The relative change in FSH concentrations was calculated. Significant differences were found for ER within 7 days of weaning in pluriparous sows (95%, 91%, and 90% for the M, F, and C groups, respectively, P ¼ 0.005). Gilts in the F-group had high TB numbers, and pluriparous sows in the M group had high SB numbers (TB gilts ¼ 13.6, 15.4, and 14.9 [P ¼ 0.02] and SB pluriparous sows ¼ 1.8, 1.4, and 1.7 [P ¼ 0.05] for the M, F, and C groups, respectively). The M group had the highest FS1 (for gilts) and FS2 (for pluriparous sows) values: FS1 ¼ 5.4, 4.9, and 4.9 mm [P ¼ 0.02] and FS2 ¼ 6.8, 5.3, and 6.3 mm [P ¼ 0.03] for the M, F, and C groups, respectively. There were no significant differences between the different treatments within each parity group with respect to any of the other variables. Overall, peforelin treatment had small but positive effects on the ER and follicle growth in certain parity groups but did not seem to affect litter sizes or FSH and progesterone levels in sows on the occasions of the corresponding examinations.

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Section: Paritymentioning

confidence: 99%

“…Increased levels of FSH during the follicular phase increase follicular size [39,40] and the size of the CL [38,41], which lead to elevated P4 levels [42].…”

Section: Paritymentioning

confidence: 99%

Effect of a GnRH analogue (Maprelin) on the reproductive performance of gilts and sows

Jong

Kauffold²,

Engl³

et al. 2013

Theriogenology

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“…were injected i.m. with a single dose of 1250 IU eCG (Folligon; Intervet, Boxmeer, The Netherlands) to promote follicular growth in 60-72 h. The dose of eCG used to stimulate follicular growth was chosen according to several reports (Mattioli et al 2001, Shimuzi et al 2002, Galeati et al 2003 since it guarantees a slight superovulatory treatment (Knox 2005). Ovulation was induced with hCG (Corulon; Intervet) treatment (750 IU, i.m.)…”

Section: Ovarian Collectionmentioning

confidence: 99%

Spatio-temporal analysis of vascular endothelial growth factor expression and blood vessel remodelling in pig ovarian follicles during the periovulatory period

Martelli¹,

Berardinelli²,

Russo³

et al. 2006

Journal of Molecular Endocrinology

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Vascular endothelial growth factor (VEGF) expression pattern and blood vessel remodelling were evaluated during the transition from the preovulatory follicle to the corpus luteum (CL). To this end, prepubertal gilts were treated with equine chorionic gonadotrophin (eCG) to collect preovulatory follicles (60 h after eCG) and with human chorionic gonadotrophin (hCG) to obtain periovulatory follicles 18 h and 36 h later. The VEGF mRNA content was analysed by in situ hybridization, while protein localization in follicular fluid (FF) and in granulosa and theca compartments was evaluated by ELISA, immunohistochemistry or western blot. Blood vessel architecture and vascular area (VA) were investigated using immunohistochemistry for von Willenbrand Factor, a specific endothelial marker. Vascular remodelling was finally tested using Ki-67 immunocytochemistry as a proliferation marker, or -smooth muscle actin ( -SMA) as a specific mural cell marker. eCG-treated follicles showed high VEGF levels and two concentric blood vessel networks composed of proliferating endothelial cells without any association with mural components. hCG injection inhibited VEGF synthesis in the granulosa compartment and, as a consequence, the protein fell within the FF. In parallel, endothelial cell proliferation stopped and the VA decreased. Close to ovulation, VEGF production restarted in both follicular compartments and VEGF mRNA content significantly increased in the theca layer. Changes in follicular VEGF secretion were observed; the protein disappeared from FF and was observed in the extracellular matrix. An active angiogenesis characterized the follicle; endothelial cell proliferation was associated with a recruitment of -SMA-positive mural cells. The data presented in this work showed that, in the phases preceding ovulation, a complete vascular remodelling occurs, characterized by both an evident neovascularization and the appearance of blood vessels presenting smooth musculature which could be involved in CL formation after ovulation.

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“…However, until now, no gene explaining a major effect on reproductive performance has been found in pigs, suggesting that this is a complex phenomenon. Possibly for this reason, authors have chosen to study the different components of reproductive performance like ovulation rate (Knox, 2005), perinatal mortality (van der Lende et al, 2001) or maternal qualities (Algers and Uvnas-Moberg, 2007) …”

Section: Introductionmentioning

confidence: 99%

A pilot study on transcriptome data analysis of folliculogenesis in pigs

Tosser‐Klopp

Cao

Bonnet

et al. 2009

Animal

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Three different stages of pig antral follicles have been studied in a granulosa-cell transcriptome analysis on nylon microarrays (1152 clones). The data have been generated from seven RNA follicle pools and several technical replicates were made. The objective of this paper was to state the feasibility of a transcriptomic protocol for the study of folliculogenesis in the pig. A statistical analysis was chosen, relying on the linear mixed model (LMM) paradigm. Low variability within technical replicates was hence checked with a LMM. Relevant genes that might be involved in the studied process were then selected. For the most significant genes, statistical methods such as principal component analysis and unsupervised hierarchical clustering were applied to assess their relevance, and a random forest analysis proved their predictive value. The selection of genes was consistent with previous studies and also allowed the identification of new genes whose role in pig folliculogenesis will be further investigated.

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Recruitment and selection of ovarian follicles for determination of ovulation rate in the pig

Cited by 92 publications

References 45 publications

Effect of a GnRH analogue (Maprelin) on the reproductive performance of gilts and sows

Effect of a GnRH analogue (Maprelin) on the reproductive performance of gilts and sows

Spatio-temporal analysis of vascular endothelial growth factor expression and blood vessel remodelling in pig ovarian follicles during the periovulatory period

A pilot study on transcriptome data analysis of folliculogenesis in pigs

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