Recovery Rate of Rat Skin Micropores Made by Dissolving Microneedles

Ito, Yoshiaki; Hamazaki, Naoki; Higashino, Hirofumi; Miyamoto, Nao; Murakami, Yuki; Inoue, Nana; Takada, Kanji

doi:10.1016/s1773-2247(13)50087-6

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…These results indicate that no damage was caused by the inflammation from the DMN application. These results were supported by our previous studies, which showed no evident inflammation and microscopic destruction of skin tissue in the microscopic image data and recovery of the rat skin after insertion of the DMNs 21 , 22 . Therefore, this device could be a noninvasive sampling method.…”

Section: Resultssupporting

confidence: 90%

Therapeutic Drug Monitoring of Vancomycin in Dermal Interstitial Fluid Using Dissolving Microneedles

Ito¹,

Inagaki²,

Kobuchi³

et al. 2016

Int. J. Med. Sci.

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Objective: To design an alternative painless method for vancomycin (VCM) monitoring by withdrawing interstitial fluid (ISF) the skin using dissolving microneedles (DMNs) and possibly replace the conventional clinical blood sampling method.Methods: Male Wistar rats were anesthetized with 50 mg/kg sodium pentobarbital. Vancomycin at 5 mg/mL in saline was intravenously administered via the jugular vein. ISF was collected from a formed pore at 15, 30, 45, 60, 75, 90, and 120 min after the DMNs was removed from the skin. In addition, 0.3 mL blood samples were collected from the left femoral vein.Results: The correlation between the plasma and ISF VCM concentrations was significantly strong (r = 0.676, p < 0.05). Microscopic observation of the skin after application of the DMNs demonstrated their safety as a device for sampling ISF.Conclusion: A novel monitoring method for VCM was developed to painlessly determine concentrations in the ISF as opposed to blood sampling.

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Section: Resultssupporting

confidence: 90%

Therapeutic Drug Monitoring of Vancomycin in Dermal Interstitial Fluid Using Dissolving Microneedles

Ito¹,

Inagaki²,

Kobuchi³

et al. 2016

Int. J. Med. Sci.

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“…Recently, skin interstitial fluid (ISF), which fills the intercellular substance of dermis, is considered as a promising alternative biofluid source because it is formed by capillary filtration through blood and thus has similar composition to that of plasma . Besides, skin ISF can be sampled through piercing microholes in the stratum corneum without damaging the blood vessels in skin tissue and can heal within several minutes, which can avoid bacterial infection . Thus, since the sampling of skin ISF involves minimal invasion and risk of infection and can also be easily self‐administered, skin ISF presents as a highly desirable sample resource for POCT.…”

Section: Introductionmentioning

confidence: 99%

A Hydrogel Microneedle Patch for Point‐of‐Care Testing Based on Skin Interstitial Fluid

Niu

et al. 2020

Adv Healthcare Materials

166

149

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Skin interstitial fluid (ISF) holds great potential as a highly desirable sample resource for point‐of‐care testing (POCT), where the key is to effectively collect target biomarkers from ISF. Hydrogel microneedle patch has great promise for ISF extraction. However, it is challenging to recover target biomarkers from the extracted skin ISF in hydrogel microneedle patches in an easy‐administrated, fast, and bulky instrument‐free manner. Herein, a hydrogel microneedle patch made of polyvinyl alcohol (PVA) and chitosan (CS) for POCT based on the ISF is developed. The phase transition property of PVA/CS hydrogel makes the microneedles stiff when dry, which is beneficial to easily penetrate into skin. Meanwhile, the highly porous microstructure of hydrogel offers good swelling ability for extraction of ISF and the thermal degradation property of PVA makes it convenient to quickly and efficiently recover target biomarkers from microneedle patch. The capability of this platform for POCT applications is further demonstrated by successful detections of typical biomarkers collected from the mimetic skin and monitoring the glucose level in rabbit skins during a day. The developed hydrogel microneedle patch holds the advantages of extraction of skin ISF and recovery of biomarkers, thus presenting as a powerful platform for skin ISF‐based POCT applications.

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“…It revealed that DMNs punctured the skin with about 200 μm depth and formed regular cone-like pinhole morphology in the skin ( Figure 4(B) ). According to previous study (Ito et al., 2013 ), the results proved DMNs we prepared could effectively penetrate the SC without touching nerves or blood vessels in the dermis.…”

Section: Resultsmentioning

confidence: 54%

Dissolving microneedle patch-assisted transdermal delivery of methotrexate improve the therapeutic efficacy of rheumatoid arthritis

et al. 2022

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Methotrexate (MTX) is a first-line treatment for rheumatoid arthritis (RA), but its clinical use is greatly limited by the adverse effects and poor patient compliance caused by traditional oral administration or injection. In recent years, some transdermal drug delivery systems have received considerable attention due to overcoming these shortcomings. In this study, we developed dissolving microneedle patch (DMNP) for transdermal delivery of MTX to treat RA safely and effectively. The morphology, mechanical strength, skin insertion, drug content, in vitro transdermal delivery, and other properties of DMNP were characterized. Meanwhile, the adjuvant-induced arthritis model of rats was established to investigate the therapeutic effect of MTX-loaded DMNP in vivo. The results showed that the microneedles had excellent morphology with neat array and complete needles, good puncture performance and mechanical strength, and rapid intradermal dissolution rate. In vitro transdermal delivery results indicated that microneedles could significantly increase drug transdermal permeation compared with the cream group. The pharmacological study showed that MTX-loaded DMNP significantly alleviated paw swelling, inhibit inflammatory response via downregulating the levels of TNF-α and IL-1β, relieved synovium destruction with less cartilage erosion, and slowed the progression of RA in AIA rats. Besides, DMNP presented better therapeutic performance than cream or intragastric administration at the same dosage of MTX. In conclusion, the MTX-loaded dissolving microneedle patch has advantages of safety, convenience, and high efficacy over conventional administrations, laying a foundation for the transdermal drug delivery system treatment of rheumatoid arthritis.

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Recovery Rate of Rat Skin Micropores Made by Dissolving Microneedles

Cited by 8 publications

References 30 publications

Therapeutic Drug Monitoring of Vancomycin in Dermal Interstitial Fluid Using Dissolving Microneedles

Therapeutic Drug Monitoring of Vancomycin in Dermal Interstitial Fluid Using Dissolving Microneedles

A Hydrogel Microneedle Patch for Point‐of‐Care Testing Based on Skin Interstitial Fluid

Dissolving microneedle patch-assisted transdermal delivery of methotrexate improve the therapeutic efficacy of rheumatoid arthritis

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