Recovery of Virus-Free Peach Trees From Selected Clones by Shoot-Tip Grafting in Vitro

Juárez, José; Arregui, J. M.; Camarasa, E.; Cambra, M.; Llácer, G.; Ortega, Cristian Valarezo; Ortega, Victoria; Navarro, Luís

doi:10.17660/actahortic.1989.235.9

Cited by 4 publications

(3 citation statements)

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“…In peach and almond, disease free plants were produced for the viruses Prunus Necrotic Ring Spot Virus (PNRSV), Prune Dwarf Virus (PDV), Apple Mosaic Virus (ApMV) and Apple Chlorotic Leaf Spot Virus (ACLSV) using the STG technology, with better results than the use of thermotherapy (Navarro et al, 1982;Juárez et al, 1988;Juárez et al, 1992). Modifi cations of the STG protocols have been eff ective for eliminating recalcitrant viruses that were not eliminated by thermotherapy and/or chemotherapy of meristem culture (Conejero et al, 2013).…”

Section: Production Of Virus-free Plants By Tissue Culturementioning

confidence: 99%

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

et al. 2013

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This review is an overview of traditional and modern breeding methodologies being used to develop new Prunus cultivars (stone fruits) with major emphasis on peach, sweet cherry and Japanese plum. To this end, common breeding tools used to produce seedlings, including in vitro culture tools, are discussed. Additionally, the mechanisms of inheritance of many important agronomical traits are described. Recent advances in stone fruit transcriptomics and genomic resources are providing an understanding of the molecular basis of phenotypic variability as well as the identifi cation of allelic variants and molecular markers. These have potential applications for understanding the genetic diversity of the Prunus species, molecular marker-assisted selection and transgenesis. Simple Sequence Repeat (SSR) and Single Nucleotide Polymorphism (SNPs) molecular markers are described as useful tools to describe genetic diversity in peach, sweet cherry and Japanese plum. Additionally, the recently sequenced peach genome and the public release of the sweet cherry genome are discussed in terms of their applicability to breeding programs.

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Section: Production Of Virus-free Plants By Tissue Culturementioning

confidence: 99%

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

et al. 2013

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“…By increasing or decreasing the size of the shoot tip from 0.7 mm, a decrease in the percent micrograft success rate was observed. Size of the scion had been shown to have a significant effect on micrograft success, with higher survival rate with larger scion size and lower with smaller scion size (Navarro 1984;Gabova et al 1988;Juarez et al 1988Juarez et al , 1992Lou and Gould 1999;Thimmappaiah et al 2002;Onay et al 2004;Suarez et al 2005). Increased success observed in the larger size shoot apex might be because of better cambial contact between the scion and the stock (Thimmappaiah et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

In vitro production of Indian citrus ringspot virus (ICRSV) free kinnow plants employing phytotherapy coupled with shoot tip grafting

Sharma

Rani

Zaidi

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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This paper reports the elimination of Indian citrus ringspot virus (ICRSV) from "kinnow" (Citrus nobilis Lour × Citrus deliciosa Tenora) employing phytotherapy coupled with shoot tip grafting under in vitro conditions. Nodal segments from infected mother plant (indexed by indirect enzyme-linked immunosorbent assay [ELISA] and reverse transcriptase PCR [RT-PCR]) were cultured on Murashige and Skoog medium containing 2iP (1 mg/l or 4.9 μM) and malt extract (800 mg/l) along with different concentrations of aqueous extracts from leaves of Azadirachta indica (Neem), Sorghum vulgare (Jowar), and roots of Boerhaavia diffusa (Punarnava). Shoot tips were excised from the nodal sprouts and grafted on to rough lemon (Citrus jambhiri) under aseptic conditions. Maximum effect (50% virus elimination) was seen for aqueous leaf extracts of A. indica followed by B. diffusa root extract (42.86%) and S. vulgare leaf extract (31.58%). Plants/plantlets were considered virus-free only when showing negative reactions by both indirect ELISA and RT-PCR.

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“…This necessitates the production of certified healthy virus free propagation material for establishing new orchards. Shoot tip grafting (STG), shoot tip/meristem culture, thermotherapy and chemotherapy have been used separately or in combination to eliminate viruses from the plants (Gabova 1988;Juarez et al 1988Juarez et al , 1992Balamuralikrishnan et al 2003;Manganaris et al 2003;Nascimento et al 2003;Ram et al 2005;Valero et al 2003;Verma et al 2005). The use of heat treatment for the elimination of ringspot viruses was first reported in stone fruit species by Nyland (1960).…”

Section: Introductionmentioning

confidence: 99%

In vitro production of Indian citrus ringspot virus (ICRSV) free Kinnow plants employing thermotherapy coupled with shoot tip grafting

Sharma

Singh

Rani

et al. 2007

Plant Cell Tiss Organ Cult

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Indian citrus ringspot virus (ICRSV) is known to cause serious disease problem in Kinnow (Citrus nobilis Lour · C. deliciosa Tenora) plants. This work reports the elimination of ICRSV by using thermotherapy coupled with shoot tip grafting in vitro. Nodal segments from infected mother plants (indexed by indirect ELISA and RT-PCR) were treated both in water bath and moist hot air at different temperatures viz. 40, 45 and 50°C for 30, 60 and 120 min and cultured on MS medium containing 2-iP (1 mg/l) and malt extract (800 mg/l). Shoot tips were excised from the nodal sprouts and grafted on to rough lemon (C. jambhiri) rootstock under aseptic conditions. Water bath treatment was found to be more effective as compared to moist hot air treatment as maximum number of ICRSV free plants (36.84%) were obtained by grafting the tips (0.7 mm) taken from the nodal segments treated at 50°C in water bath for 2 h. In an alternate treatment regime, 1-year-old infected plants were kept at various temperatures viz.36, 38 and 40°C in a thermotherapy chamber. Maximum of 60% ICRSV free plants were obtained by grafting the tips (0.7 mm) from the plants placed at 40°C followed by the plants placed at 38°C (59.09%) and the least was observed in case of the plants placed at 36°C (40.74%). Only those plants/plantlets were considered virus free, which showed negative reaction both in Indirect ELISA and RT-PCR.

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Recovery of Virus-Free Peach Trees From Selected Clones by Shoot-Tip Grafting in Vitro

Cited by 4 publications

References 0 publications

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

Breeding in peach, cherry and plum: from a tissue culture, genetic, transcriptomic and genomic perspective

In vitro production of Indian citrus ringspot virus (ICRSV) free kinnow plants employing phytotherapy coupled with shoot tip grafting

In vitro production of Indian citrus ringspot virus (ICRSV) free Kinnow plants employing thermotherapy coupled with shoot tip grafting

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