Recovery of Vibrio harveyi from scale drop and muscle necrosis disease in farmed barramundi, Lates calcarifer in Vietnam

Dong, Ha Thanh; Taengphu, S; Sangsuriya, Pakkakul; Charoensapsri, Walaiporn; Phiwsaiya, Kornsunee; Sornwatana, Thakorn; Khunrae, Pongsak; Rattanarojpong, Triwit; Senapin, Saengchan

doi:10.1016/j.aquaculture.2017.02.005

Cited by 81 publications

(46 citation statements)

References 28 publications

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“…Dong, Taengphu et al. () performed experimental challenges with several bacterial species recovered from naturally diseased fish and found that only V. harveyi fulfilled Koch's postulates. The novel histopathological changes of the kidney (e.g., collapsed kidney tubules and sloughing of epithelial cells) observed in both naturally and experimentally diseased fish suggested possible involvement of V. harveyi toxins.…”

Section: Discussionmentioning

confidence: 99%

“…It is widely accepted that gross signs are useful for presumptive diagnosis, but this needs to be confirmed by more specific histological, microbiological or molecular methods. The gross sign of scale loss ("scale drop") occurs not only with SDDV-infected fish but also with other diseases in farmed sea bass such as Tenacibaculosis caused by T. maritimum (Avendano-Herrera, Toranzo, & Magarinos, 2006) and "scale drop and muscle necrosis disease (SDMND)" in Vietnamese sea bass farms (Dong, Taengphu et al, 2017). The latter case involved multiple infections of both culturable and unculturable bacteria, and a unique isolate of Vibrio harveyi was found to play a major role (Dong, Taengphu et al, 2017;Kayansamruaj et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The latter case involved multiple infections of both culturable and unculturable bacteria, and a unique isolate of Vibrio harveyi was found to play a major role (Dong, Taengphu et al, 2017;Kayansamruaj et al, 2018). Dong, Taengphu et al (2017) done to identify the tissue of highest viral load for diagnostic purposes.…”

Section: Discussionmentioning

confidence: 99%

“…DNA was extracted from the kidney and liver of individual fish collected between 2016 and 2018 using lysis buffer containing proteinase K as previously described (Sangsuriya et al., ) followed by conventional DNA extraction using phenol–chloroform and ethanol precipitation (Sambrook & Russell, ). Similar DNA extracts originating from clinically healthy sea bass from a previous study (Dong, Taengphu et al., ) were included as negative controls. Extracted DNA was spectrophotometrically measured and adjusted to 100 ng/μl for PCR amplification.…”

Section: Methodsmentioning

confidence: 99%

“…One emerging disease in farmed L. calcarifer in Southeast Asia is scale drop disease (SDD) caused by a novel Megalocytivirus (SDDV) (De Groof et al., ; Gibson‐Kueh et al., ). Another is scale drop and muscle necrosis (SDMN) disease associated with a pathogenic Vibrio harveyi isolate (Dong, Taengphu et al., ; Kayansamruaj et al., ). Yet another is infectious spleen and kidney necrosis disease (ISKND) caused by the virus called ISKNV genotype II (Dong, Jitrakorn et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia

Senapin

Dong

Meemetta

et al. 2018

Journal of Fish Diseases

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In Southeast Asia, a new disease called scale drop disease (SDD) caused by a novel Megalocytivirus (SDDV) has emerged in farmed Asian sea bass (Lates calcarifer) in Singapore, Malaysia and Indonesia. We received samples from an Eastern Thai province that also showed gross signs of SDD (loss of scales). Clinical samples of 0.2–1.1 kg L. calcarifer collected between 2016 and 2018 were examined for evidence of SDDV infection. Histopathology was similar to that in the first report of SDDV from Singapore including necrosis, inflammation and nuclear pyknosis and karyorrhexis in the multiple organs. Intracytoplasmic inclusion bodies were also observed in the muscle tissue. In a density‐gradient fraction from muscle extracts, TEM revealed enveloped, hexagonal megalocytiviral‐like particles (~100–180 nm). By PCR using primers derived from the Singaporean SDDV genome sequence, four different genes were amplified and sequenced from the Thai isolate revealing 98.7%–99.9% identity between the two isolates. Since viral inclusions were rarely observed, clinical signs and histopathology could not be used to easily distinguish between SDD caused by bacteria or SDDV. We therefore recommend that PCR screening be used to monitor broodstock, fry and grow‐out fish to estimate the current impact of SDDV in Southeast Asia and to prevent its spread.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia

Senapin

Dong

Meemetta

et al. 2018

Journal of Fish Diseases

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Natural Concurrent Infection of Vibrio harveyi and V. alginolyticus in Cultured Hybrid Groupers in Malaysia

et al. 2019

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In September 2016, a marine fish farm operator in Selangor, Malaysia, reported a disease outbreak affecting juvenile hybrid groupers (Camouflage Grouper Epinephelus polyphekadion × Tiger Grouper E. fuscoguttatus). The average daily mortality was 120 fish, resulting in a cumulative mortality rate of 29% within 10 d. The affected hybrid groupers displayed lethargy, excessive mucus production, rotten fins, congestion of livers and kidneys, and enlargement of spleens. Microscopically, general congestion of the brains and internal organs was evident. Vibrio harveyi and V. alginolyticus were successfully isolated from the diseased fish. The isolated pathogens were found to be sensitive to oxytetracycline and tetracycline, but resistant towards ampicillin and vancomycin. Experimental infections using the isolated V. harveyi (10 8 CFU/mL), V. alginolyticus (10 8 CFU/mL), and concurrent infection by V. harveyi (10 8 CFU/mL) and V. alginolyticus (10 8 CFU/mL) in juvenile Asian Seabass Lates calcarifer resulted in 60, 100, and 100% mortality, respectively, within 240 h postinfection. The experimentally infected Asian Seabass demonstrated similar clinical signs and histopathological changes as the naturally infected hybrid groupers. However, concurrently infected

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Environmental Factors Associated with the Presence of Vibrionaceae in Tropical Cage‐Cultured Marine Fishes

et al. 2019

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This study investigated the environmental factors associated with the presence of Vibrionaceae in economically important cage‐cultured tropical marine fishes: the Asian Seabass Lates calcarifer, snapper Lutjanus sp., and hybrid grouper Epinephelus sp. Fish sampling was conducted at monthly intervals between December 2016 and August 2017. The body weight and length of individual fish were measured, and the skin, eye, liver, and kidney were sampled for bacterial isolation and identification. Water physicochemical parameters during the sampling activities were determined, and the enumeration of total Vibrionaceae count was also conducted from water and sediment samples. Nine species of Vibrio were identified, including V. alginolyticus, V. diabolicus, V. harveyi, V. campbellii, V. parahaemolyticus, V. rotiferianus, V. furnissii, V. fluvialis, and V. vulnificus. Photobacterium damselae subsp. damselae was also identified. A total of 73% of the isolated Vibrio belonged to the Harveyi clade, followed by the Vulnificus clade (5.5%) and Cholera clade (0.6%). Highest occurrence of Vibrio spp. and P. damselae subsp. damselae was found in hybrid grouper (72%), followed by Asian Seabass (48%) and snapper (36%). The associations of Vibrio spp. and P. damselae subsp. damselae with the host fish were not species specific. However, fish mortality and fish size showed strong associations with the presence of some Vibrio spp. On average, 60% of the infected cultured fish exhibited at least one clinical sign. Nevertheless, inconsistent associations were observed between the pathogens and water quality. The yearlong occurrence and abundance of Vibrionaceae in the environmental components indicate that they might serve as reservoirs of these pathogens.

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Recovery of Vibrio harveyi from scale drop and muscle necrosis disease in farmed barramundi, Lates calcarifer in Vietnam

Cited by 81 publications

References 28 publications

Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia

Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia

Natural Concurrent Infection of Vibrio harveyi and V. alginolyticus in Cultured Hybrid Groupers in Malaysia

Environmental Factors Associated with the Presence of Vibrionaceae in Tropical Cage‐Cultured Marine Fishes

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