“…A small aliquot was taken for routine testing and counts including WCC, blood group confirmation, microbial studies and viable CD34 and CD3 cell number enumeration by flow cytometry, using a modified single platform ISHAGE gating method. 10,11 Antibody panel includes: CD34-PE (clone 8G12; Becton Dickinson, Franklin Lakes, NJ, USA), CD45-FITC (clone 2D1; Becton Dickinson) and 7-AAD (Becton Dickinson). CD3-APC (clone SK7; Becton Dickinson) was also routinely included in the antibody panel for marrow harvests starting in 2014.…”