Recovery of vestibular function in young guinea pigs after streptomycin treatment. Glutamate decarboxylase activity and nystagmus response assessment

Meza, Graciela; Solano-Flores, Luis Pastor; Poblano, Adrián

doi:10.1016/0736-5748(92)90030-4

Cited by 13 publications

(9 citation statements)

References 14 publications

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“…Damaged, but surviving, hair cells may be found after systemic treatments where the levels of aminoglycosides are sufficiently weak as to not kill the animals from nephrotoxicity. Taken together, the observations suggest the possibility that loss and replacement of hair bundles may occur in humans and could potentially contribute to previously unexplained delayed recovery of auditory and vestibular sensitivity in mammals after treatments with ototoxic antibiotics (Black et al, 1987;Nicol et al, 1992;Meza et al, 1992). Such cellular repair might also explain delayed recovery of hearing thresholds after certain types of noise exposure, a phenomenon that is reported to continue for days (Mills et al, 1970).…”

Section: Discussionmentioning

confidence: 76%

Survival of bundleless hair cells and subsequent bundle replacement in the bullfrog's saccule

et al. 2002

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Our senses of hearing and balance depend upon hair cells, the sensory receptors of the inner ear. Millions of people suffer from hearing and balance deficits caused by damage to hair cells as a result of exposure to noise, aminoglycoside antibiotics, and antitumor drugs. In some species such damage can be reversed through the production of new cells. This proliferative response is limited in mammals but it has been hypothesized that damaged hair cells might survive and undergo intracellular repair. We examined the fate of bullfrog saccular hair cells after exposure to a low dose of the aminoglycoside antibiotic gentamicin to determine whether hair cells could survive such treatment and subsequently be repaired. In organ cultures of the bullfrog saccule a combination of time-lapse video microscopy, two-photon microscopy, electron microscopy, and immunocytochemistry showed that hair cells can lose their hair bundle and survive as bundleless cells for at least 1 week. Time-lapse and electron microscopy revealed stages in the separation of the bundle from the cell body. Scanning electron microscopy (SEM) of cultures fixed 2, 4, and 7 days after antibiotic treatment showed that numerous new hair bundles were produced between 4 and 7 days of culture. Further examination revealed hair cells with small repaired hair bundles alongside damaged remnants of larger surviving bundles. The results indicate that sensory hair cells can undergo intracellular self-repair in the absence of mitosis, offering new possibilities for functional hair cell recovery and an explanation for non-proliferative recovery.

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Section: Discussionmentioning

confidence: 76%

Survival of bundleless hair cells and subsequent bundle replacement in the bullfrog's saccule

et al. 2002

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“…In mammals, although morphological evidence is available pointing to regeneration of hair cells Warchol et al, 1993;Sobkowicz et al, 1996;Lopez et al, 1997;Zheng et al, 1999] functional recovery was not confirmed using direct end-organ parameters. Studies that had shown functional recovery after ototoxic treatment used functional parameters that were (partially) based on central compensation [Meza et al, 1992[Meza et al, , 1996Taura et al, 2006;Staecker et al, 2007]. In this study we used parameters of direct end-organ function, so the effects of central compensation did not bias the longerterm results.…”

Section: Long-term Effect Of Gentamicin Treatmentmentioning

confidence: 99%

“…In a recent study where type-I hair cell regeneration was induced with Math1 gene transfer, it was shown that the appearance of the new type-I hair cells was not normal [Xu et al, 2012]. Whether vestibular function may recover in mammals as a result of hair cell regeneration is thus an important question, and this has been addressed by only a few studies [Meza et al, 1992[Meza et al, , 1996Taura et al, 2006]. Meza et al [1992] showed recovery of vestibular function in guinea pigs treated with streptomycin by assessing nystagmus responses.…”

mentioning

confidence: 99%

“…Whether vestibular function may recover in mammals as a result of hair cell regeneration is thus an important question, and this has been addressed by only a few studies [Meza et al, 1992[Meza et al, , 1996Taura et al, 2006]. Meza et al [1992] showed recovery of vestibular function in guinea pigs treated with streptomycin by assessing nystagmus responses. Meza et al [1996] studied damage and recovery of otolith function in the rat after streptomycin treatment by swimming behaviour.…”

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confidence: 99%

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Does Vestibular End-Organ Function Recover after Gentamicin-Induced Trauma in Guinea Pigs?

Bremer

Versnel²,

Hendriksen³

et al. 2014

Audiol Neurotol

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Until 1993 it was commonly accepted that regeneration of vestibular hair cells was not possible in mammals. Two histological studies then showed structural evidence for spontaneous regeneration of vestibular hair cells after gentamicin treatment. There is less evidence for functional recovery going along with this regenerative process; in other words, do regenerated hair cells function adequately? This study aims to address this question, and in general evaluates whether spontaneous functional recovery may occur, in the short or long term, in mammals after ototoxic insult. Guinea pigs were treated with gentamicin for 10 consecutive days at a daily dose of 125 mg/kg body weight. Survival times varied from 1 day to 16 weeks. Vestibular short-latency evoked potentials (VsEPs) to linear acceleration pulses were recorded longitudinally to assess otolith function. After the final functional measurements we performed immunofluorescence histology for hair cell counts. Auditory brainstem responses (ABRs) to click stimuli were recorded to assess cochlear function. As intended, gentamicin treatment resulted in significant loss of utricular hair cells and accompanying declines in VsEPs. Hair cell counts 8 or 16 weeks after treatment did not significantly differ from counts after shorter survival periods. Maximal functional loss was achieved 1-4 weeks after treatment. After this period, only 2 animals showed recovery of VsEP amplitude - all other animals did not reveal signs of regeneration or recovery. In contrast, after initial ABR threshold shifts there was a small but significant recovery. We conclude that spontaneous recovery of otolith function, in contrast to cochlear function, is very limited in guinea pigs. These results support the concept of intratympanic gentamicin treatment where gentamicin is used for chemoablation of the vestibular sensory epithelia.

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“…It has been suggested for some time that aminoglycoside drugs are ototoxic [2]. Data from reports of research in animals [11] and some studies carried on in human infants support these results [1,4]. Data from reports of research in animals [11] and some studies carried on in human infants support these results [1,4].…”

Section: Introductionmentioning

confidence: 97%

Amikacin alters auditory brainstem conduction time in newborns

Poblano¹,

Belmont²,

Sosa³

et al. 2003

Journal of Perinatal Medicine

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The purpose of this paper was to describe whether there are some relationships between amikacin serum levels and central conduction time in brainstem auditory evoked potentials (BAEP) within therapeutic range levels in newborns as index of drug toxicity in brainstem auditory centers in neonatally exposed infants. We performed a cross-sectional study to compare BAEP from 35 infants under amikacin administration and 24 control infants; both examinations were blinded to investigators. Bivariate and partial correlations were calculated between amikacin and BAEP measurements in treated infants. Amikacin determinations were within therapeutic levels. No clinical alterations in BAEP were found and no differences between amikacin-treated and control infants were found. Significant positive Pearson correlation between latency of I-III interwaves interval and amikacin Cmin serum levels was found and was present when calculations were controlled by partial correlations for gestational age at birth and Apgar score at 5 min. The findings suggest that increased amikacin levels in newborns are related to increased latencies in I-III interwave interval in infants, which may be an early index of brainstem effects of subclinical neurotoxicity of amikacin.

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Recovery of vestibular function in young guinea pigs after streptomycin treatment. Glutamate decarboxylase activity and nystagmus response assessment

Cited by 13 publications

References 14 publications

Survival of bundleless hair cells and subsequent bundle replacement in the bullfrog's saccule

Survival of bundleless hair cells and subsequent bundle replacement in the bullfrog's saccule

Does Vestibular End-Organ Function Recover after Gentamicin-Induced Trauma in Guinea Pigs?

Amikacin alters auditory brainstem conduction time in newborns

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