Recovery of valuable compounds from orange processing wastes using supercritical carbon dioxide extraction

Enzyme-Assisted Extraction of Nuciferine and Quercetin from Lotus Leaves Using the Aqueous-Based Method: A Sustainable Approach

“…The TFC values of the sample or extract were determined according to Argun . The sample was reacted sequentially with NaNO 2 and AlCl 3 to form a flavonoid–aluminum complex.…”

“…The total alkaloid content (TAC) of the lotus leaf extract was determined according to the method described by Argun with minor modifications. 10 μL of the lotus leaf extract concentrate/standard solution was diluted to 5 mL with trichloromethane (chloroform), and 1 mL of potassium hydrogen phthalate buffer (pH 4) and 1 mL of bromocresol green solution were added . The mixture was shaken, then 5 mL of chloroform was added and shaken vigorously after mixing.…”

“…The TFC values of the sample or extract were determined according to Argun. 34 The sample was reacted sequentially with NaNO 2 and AlCl 3 to form a flavonoid−aluminum complex. The calibration curve was prepared using standard solutions of quercetin, and absorbance was measured at 510 nm.…”

“…10 μL of the lotus leaf extract concentrate/standard solution was diluted to 5 mL with trichloromethane (chloroform), and 1 mL of potassium hydrogen phthalate buffer (pH 4) and 1 mL of bromocresol green solution were added. 34 The mixture was shaken, then 5 mL of chloroform was added and shaken vigorously after mixing. The mixture was kept at room temperature for 30 min.…”

Insight into Green Extraction for Roselle as a Source of Natural Red Pigments: A Review

“…Supercritical carbon dioxide (ScCO 2 ) is often used to extract bioactive compounds, such as phenolic, flavonoid, and antioxidant compounds [ 16 , 17 , 18 ]. This method is a novel approach for optimizing the extraction of chemicals of interest, especially for anthocyanins since it is a non-hazardous and eco-friendly solvent.…”