Ferruginous sandstone considered as a one of the economically significant uranium mineralized areas in the southwestern part of the Sinai that located at the lower member of Um Bogma Formation in Abu Thor area. The studied sample was characterized geologically as a sandstone rock contain 450 mg/kg of uranium. This work is aimed to compare between eco-friendly bioleaching and conventional leaching processe. The uranium bioleaching efficiency achieved 80% by using a native fungal strain; Aspergillus nidulans as a green technology with the optimimum conditions as; 3 pH value, 3% pulp density, 7 days incubation period, and 30 °C incubation temperatures. Whereas the traditional technique using alkaline and sulphuric acid leaching attained 68.5% and 95% respectively at the best condition. Finally, the shrinking core model was represented the layer diffusion process by acquiring apparent activation energy 18.28 and 16.82 kJ/mol for carbonate and sulphuric acid leaching, respectively.