Recovery of the membrane potential of the sectioned striated muscle fibers of the guinea-pig cremaster.

Ninomiya, Jesús G.; Mascher, Dieter

doi:10.2170/jjphysiol.32.529

Cited by 2 publications

(4 citation statements)

References 22 publications

(23 reference statements)

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“…The time course of the recovery of membrane potential found in the present study with an electrode situated at 100 or 200 jam from the lesion matches closely that registered at 0.5 mm from the injury by Ninomiya and Mascher [1982]. This correspondence suggests that the diffusion barrier does not form at the front of the penetration of the extracellular medium, i.e.. 350 jum inside the fiber [Echeverría etal.. 1983], but at the lesioned surface.…”

Section: Discussionsupporting

confidence: 86%

“…1982] , support the former hypothesis. Furukawa [ 1984] has confirmed some of our previous results in regard to the healing-over of the cremaster muscle and the lack of this process in the diaphragm [Ninomiya and Mascher. 1982].…”

Section: Discussionsupporting

confidence: 85%

“…The membrane potential of intact striated fibers of the cremaster muscle of the guinea pig is -77.5 ±5.9 mV [Ninomiya and Mascher, 1982], After 1 h in Krebs' solu tion in vivo their membrane potential is -75 ±5.4 mV [Alonso-de Florida et al. 1972: Ninomiya and Mascher.…”

Section: Eleclrophysiological Measurementsmentioning

confidence: 99%

“…Moreover, the cremaster muscle presents a calciumdependent recuperation of membrane potential after tran section of its striated fibers, probably due to the formation of a new barrier to diffusion which prevents the flow of material at the cut end. Ninomiya and Mascher [1982] hypothesized that membrane sealing and plug formation play an important role in the healing-over in the injured cremaster muscle. Morphological studies provided partial evidence supporting this view [Echeverría et al, 1983].…”

Section: Introductionmentioning

confidence: 99%

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Microscopical and Electrophysiological Studies on the Healing-Over of Striated Fibers of Cremaster Muscle of the Guinea Pig

Echeverría¹,

Ninomiya²,

Vázquez‐Nin³

1987

Cells Tissues Organs

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In previous works it has been shown that the striated fibers of the cremaster muscle undergo a calcium-dependent healing-over reaction after transection and that the fibers of the diaphragm under the same conditions do not recover. In the present work striated fibers of the cremaster and diaphragm are studied, using electrophysiological techniques, light and electron microscopy, 15, 30, 45 and 6.0 min after transection in an attempt to clarify the process leading to the recovery of the fibers of the cremaster muscle. The recording of membrane potentials at different times after the lesion in the immediate vicinity of the cut end demonstrates that the new diffusion barrier is formed at the damaged surface. Light microscopy of fibers of cremaster transected in vitro indicates the existence of a rapid process preventing the outflow of particulate constituents of the cytoplasm 1 min after the lesion. Electron microscopy shows that this hindrance is due to a compact disposition of filaments derived from altered myofibrils near the cut end. This filamentary plug does not exist in the diaphragm. Cell membrane closing is a slow phenomenon which is completed between 30 and 60 min after the lesion in different fibers. No reconstitution of the cell membrane was found in the fibers of the diaphragm. Rapid and slow responses are interpreted as particular cases of the surface precipitation reaction known in several cell types for more than 40 years.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 85%

Section: Eleclrophysiological Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microscopical and Electrophysiological Studies on the Healing-Over of Striated Fibers of Cremaster Muscle of the Guinea Pig

Echeverría¹,

Ninomiya²,

Vázquez‐Nin³

1987

Cells Tissues Organs

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Healing‐over in skeletal muscles of the guinea pig following induced injury

Furukawa

1984

Muscle and Nerve

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Electrical membrane properties of cremaster muscle fibers of the guinea pig were determined before and after sectioning, using the microelectrode method. After sectioning, the membrane was depolarized to a maximum level within 5 minutes and the original level was reverted to within 60 minutes. Following restoration of the membrane potential, the spatial distribution of electrotonic potential indicated an electrical sealing at the cut end, and an action potential could be evoked. Therefore, it would appear that the cremaster muscle possesses the healing-over phenomenon. This process was dependent on temperature and [Ca2+]o and was not affected by pretreatment of actinomycin D, a protein synthesis inhibitor. This healing-over process also occurs in the extensor digitorum longus (EDL) muscle of the guinea pig but not in the diaphragm. These results indicate that some mammalian skeletal muscle fibers possess the healing-over phenomenon, as is observed in cardiac muscle fibers.

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Recovery of the membrane potential of the sectioned striated muscle fibers of the guinea-pig cremaster.

Abstract: We investigated changes in membrane potential after small

Cited by 2 publications

References 22 publications

Microscopical and Electrophysiological Studies on the Healing-Over of Striated Fibers of Cremaster Muscle of the Guinea Pig

Microscopical and Electrophysiological Studies on the Healing-Over of Striated Fibers of Cremaster Muscle of the Guinea Pig

Healing‐over in skeletal muscles of the guinea pig following induced injury

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