Recovery of the Hepatic Carcinogen-Metabolizing Capacity in Schistosome-Infected Mice After Treatment With the Antischistosomal Praziquantel

Sheweita, Salah A.; Mostafa, M.

doi:10.3892/or.2.1.155

Cited by 10 publications

(18 citation statements)

References 14 publications

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“…In mice, S. mansoni increased the activity of various drug-metabolizing enzymes, including cytochrome P450, cytochrome b 5 , NADPH-cytochrome c reductase, aryl hydrocarbon hydroxylase, and dimethylnitrosamine N-demethylase I (DMN-dI), at earlier stages of schistosomal infection; at later stages of infection, these activities subsided again [9,21]. In addition, cytochrome P450 content has also been decreased in S. mansoni-infected human livers [8,22].…”

Section: Introductionmentioning

confidence: 96%

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Changes in expression and activity of glutathione S‐transferase in different organs of schistosoma haematobium‐infected hamster

Sheweita

Mostafa

Ebid

et al. 2003

J Biochem & Molecular Tox

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Schistosomiasis is a major health problem in many subtropical developing countries, causing a number of serious pathologies, including bladder cancer. Most of the toxic compounds formed as a result of these infestations are derived either exogenously or formed endogenously and can be conjugated with glutathione (GSH) via glutathione S-transferase (GST). The present study investigates the effect of Schistosma haematobium infection on the activity of GST and glutathione reductase (GR) and levels of glutathione and free radicals (measured as thiobarbituric acid reactive substances) in different organs of the male hamster. The total activity of GST was increased in several organs; in kidney by 50 and 46% at 6 and 10 weeks postinfection, respectively, and in bladder tissues by 169, 23, and 130% at 2, 4, and 6 weeks postinfection, respectively. In support of this, the expression of GST isozymes was also induced in kidney and bladder tissues at early stages (2, 4, and 6 weeks) and reduced at the later stages of infection (8 and 10 weeks). In contrast, the expression of these isozymes was decreased in the spleen and liver at 2, 4, 6, 8, and 10 weeks postinfection. Also, such activity was decreased in lungs by 74 and 78% and in bladders by 65 and 72% at 8 and 10 weeks postinfection, respectively. GSH levels increased in lungs by 95, 40, and 56% at 2, 4, and 6 weeks and in spleen by 26 and 74% at 4 and 6 weeks, respectively, but decreased at later stages of S. haematobium infection in these organs. The depletion of GSH levels also occurred in bladders by 72 and 54% at 8 and 10 weeks postinfection, respectively. The activity of GR was increased in the livers, lungs, and kidneys of the S. haematobium-infected hamster. TBARS also increased in the lung by 14, 65, 53, 828, and 624% and in the kidney by 64, 29, 87, 190, and 111%, and in the bladder by 216, 23, 1468, 528, and 1025% at 2, 4, 6, 8, and 10 weeks postinfection, respectively. This study indicates that low GST expression and high levels of free radicals could provide new evidence for damage to the bladder and other organs as a result of S. haematobium infection.

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Section: Introductionmentioning

confidence: 96%

“…Previous studies have demonstrated the influence of S. mansoni on the hepatic content of cytochrome P450 and other drug-metabolizing enzymes after different periods of infection in the liver of male mice [9,21,26]. No previous studies have been conducted on hamster tissues under the influence of S. haematobium.…”

Section: Introductionmentioning

confidence: 99%

Changes in expression and activity of glutathione S‐transferase in different organs of schistosoma haematobium‐infected hamster

Sheweita

Mostafa

Ebid

et al. 2003

J Biochem & Molecular Tox

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“…The mycelia were removed, chilled on ice, washed with phosphate buffer (pH 7) and homogenized separately in 3 volume in the same buffer. The crude homogenate was centrifuged at 10 000 × g for 10 minutes at 4°C (Sheweita and Mostafa 1995). The supernatant fraction was used as the enzyme source.…”

Section: Methodsmentioning

confidence: 99%

Gibberellin and auxin production by plant root-fungi and their biosynthesis under salinity-calcium interaction

Hasan¹

2002

Plant Soil Environ.

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Rhizosphere and rhizoplane of fababean (Vicia faba), melochia (Corchorus olitorius), sesame (Sesamum indicum) and soyabean (Glycine max) plants are inhabited with fungi, mostly Aspergillus flavus, A. niger, Fusarium oxysporum, Penicillium corylophilum, P. cyclopium, P. funiculosum and Rhizopus stolonifer. All fungal species have the ability to produce gibberellin (GA) but F. oxysporum was found to produce both GA and indole-acetic acid (IAA). The optimum period for GA and IAA production by F. oxysporum was 10 days in the mycelium and 15 days in the filtrate at 28°C. The contents of GA and IAA were significantly increased at 0.5 and 1% NaCl after 5 days, but they were lowered at 4% (700 mM) NaCl. Cytochrome P-450 was significantly increased under salt stress at 0.5-7% NaCl. Calcium decreased NaCl stress on F. oxysporum by significant elevating GA biosynthesis at 40 mM Ca 2+ /700 mM Na + . GA at 10 µM and Ca 2+ at 10 mM enhanced the germination of seeds under 175 mM Na + .Keywords: GA; IAA; cytochrome; fungi; calcium; salinityThe microbial synthesis of plant growth regulators (gibberellin and auxin) is an important factor in soil fertility (Kampert and Strzelczyk 1975). Gibberellin (GA) and indole-acetic acid (IAA) are secondary metabolites, which are important biotechnological products, produced commercially from fungi for the agricultural and horticultural industry (Deacon 1984, Bruckner 1992. GA was isolated from Gibberella fujikuroi by T. Yabuta and IAA was isolated from Rhizopus suinus by K.V. Thimann in 1935. GA 3 is the dominant component of the gibberellin complexes isolated from fungi (Bozhkova et al. 1991). It is now generally agreed that GA and IAA are very widely distributed throughout the plant kingdom.Salinity represents one of the most important factors exerting stress on fungal as well as plant cell metabolism. Kaur et al. (1998) found that GA 3 at 6µM concentration induced increased germination and seedling growth under salt stress. Calcium is particularly important nutrient in plants exposed to NaCl stress because of its role in maintaining the structural and functional integrity of membrane (Leopold and Willing 1984) and in cell wall extensibility (Taiz 1984).No recent study explains the ability of filamentous fungi, associated with roots of plant crops, for the GA and IAA production and their role toward the host plant crops. Also, no studies explain the effect of salinity on the activity of rhizosphere and rhizoplane fungi and role of Ca 2+ and GA in alleviation the salt stress. The present work was commenced by collecting four plant crops for isolation of both rhizosphere and rhizoplane fungi, and screening their efficiencies for GA and IAA production. Also, effect of salinity on GA, IAA and cytochrome P-450 biosynthesis by F. oxysporum was studied. The roles of GA and Ca 2+ in reversing the toxicity of salt stress effect in seedlings were also studied. MATERIAL AND METHODSCollection of samples. Ten samples of fababean (Vicia faba), melochia (Corchorus olitorius), sesame (Sesamum ind...

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“…It has been demonstrated that S. mansoni infection increased the activity of drug-metabolizing enzymes in the liver including P-450, cytochrome b5, and NADPH-cytochrome C reductase at earlier stages (30 days) of schistosomal infection; at later stages of infection (75 days), these activities subsided again [78]. The decrease in the activity of drug-metabolizing enzymes in the liver of humans and experimental animals in the later stages of the disease might be related to the development of liver fibrosis or to toxic metabolites produced either by adult S. mansoni worms or their deposited ova [79,80].…”

Section: Disturbed Carcinogen Activation In Schistosomiasismentioning

confidence: 99%

“…Most aromatic amines are initially activated by N-hydroxylation, mainly in the liver via a cytochrome P-450 catalyzed reaction [88,89] which is influenced by Schistosomiasis [78]. Thus the effect of Schistosomiasis on the metabolic activation of amines might be similar to those of polycyclic aromatic hydrocarbons.…”

Section: Disturbed Carcinogen Activation In Schistosomiasismentioning

confidence: 99%

Schistosomiasis and Neoplasia

Yosry

2006

Infection and Inflammation: Impacts on Oncogenesis

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Schistosomiasis is endemic in at least 75 tropical and subtropical countries where 600 million people are at risk of which over 200 million are infected. Three species, S. hematobium, S. mansoni and S. japonicum, account for the majority of human infections. There is sufficient evidence that S. hematobium, the predominant etiologic agent for urinary schistosomiasis, is carcinogenic to humans leading to squamous cell carcinoma of the urinary bladder, a relatively uncommon vesical cancer in nonendemic areas. There is limited evidence suggesting that S. japonicum is possibly carcinogenic to humans leading to colorectal cancer and is a risk factor for hepatocellular carcinoma formation. There is inadequate evidence for the carcinogenicity of S. mansoni in humans. S. mansoni may still be linked to hepatocellular carcinoma through potentiating the effects of hepatitis B virus and hepatitis C virus on the liver. In this article, the relationship between schistosomiasis and neoplasia will be reviewed.

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Recovery of the Hepatic Carcinogen-Metabolizing Capacity in Schistosome-Infected Mice After Treatment With the Antischistosomal Praziquantel

Cited by 10 publications

References 14 publications

Changes in expression and activity of glutathione S‐transferase in different organs of schistosoma haematobium‐infected hamster

Changes in expression and activity of glutathione S‐transferase in different organs of schistosoma haematobium‐infected hamster

Gibberellin and auxin production by plant root-fungi and their biosynthesis under salinity-calcium interaction

Schistosomiasis and Neoplasia

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