Recovery of the Gut Microbiota after Antibiotics Depends on Host Diet, Community Context, and Environmental Reservoirs

Ng, Katharine M.; Aranda-Díaz, Andrés; Tropini, Carolina; Frankel, Matthew Ryan; Treuren, William Van; O’Loughlin, Colleen T.; Merrill, Bryan D.; Yu, Feiqiao Brian; Pruss, Kali M.; Oliveira, Rita Almeida; Higginbottom, Steven K.; Neff, Norma; Fischbach, Michael A.; Xavier, Karina B.; Sonnenburg, Justin L.; Huang, Kerwyn Casey

doi:10.1016/j.chom.2020.09.001

Cited by 48 publications

(84 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The alpha diversity of gut microbiota in aged mice did not recover to the baseline level, suggesting the elimination of community members during the antibiotic treatment (Ng et al, 2020). These observations are consistent with previous studies in young mice (Suez et al, 2018) and in an independent experiment that we carried out in 2-month-old mice ( Figure S1).…”

Section: Spontaneous Recovery Of Gut Microbiota Following Antibiotic supporting

confidence: 91%

Establishment and Resilience of Transplanted Gut Microbiota in Aged Mice

Wang

Tang

et al. 2021

Preprint

View full text Add to dashboard Cite

Fecal microbiota transplantation (FMT), a procedure in which fecal material is transferred from a donor to a recipient, has been increasingly used as a treatment to restore healthy gut microbiota. There is a substantial difference in the composition of gut microbiota between young and aged hosts, but little is known about whether age matching between the FMT donor and recipient affects microbiota restoration and long-term maintenance. In the present investigation, we aimed to study the establishment and resilience of transplanted gut microbiota in aged recipients. We treated naturally aged mice (20 months old) with a broad-spectrum antibiotic cocktail and monitored the restoration of gut microbiota over 8 weeks. The diversity of gut microbiota in aged mice failed to reach the baseline level via spontaneous recovery; in contrast, FMT from either (age-)matched or unmatched donors facilitated the recovery of gut microbiota diversity. The microbiota transplanted from different donors successfully established in the aged recipients and had long-term effects on the gene expression profiles of the host colon. Finally, we evaluated the long-term maintenance of transplanted microbiota via intentional disruption of gut homeostasis. We found that lack of age matching between FMT donors and recipients may decrease the resilience of transplanted gut microbiota against colonic inflammation. The results from our study systematically examining the effects of FMT on the gut homeostasis of aged hosts suggest that the compatibility between donors and recipients should be taken into account when implementing FMT.

show abstract

Section: Spontaneous Recovery Of Gut Microbiota Following Antibiotic supporting

confidence: 91%

Establishment and Resilience of Transplanted Gut Microbiota in Aged Mice

Wang

Tang

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The ability of the gut microbiota to recover to the baseline level after stopping antibiotics may vary depending on differences in the antibiotic administration, the host species, the community context, and environmental reservoirs [2,22]. In the present study, the gentamicin bath signi cantly changed the composition of the gut bacterial community; however, after 7 days, the gut microbiota was still similar to that during the gentamicin bath.…”

Section: Variation In Gut Microbiota Diversitymentioning

confidence: 47%

Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

Tong

Cui

Jia

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Amphibians frequently receive an antibiotic bath after feedlot placement to control bacterial diseases. The potential collateral effect of these antibiotics on the frog microbiota is largely unknown. Antibiotics are frequently employed to examine the role of the gut microbiota. Existing research relies mainly on oral antibiotics, but knowledge regarding the effects of antibiotics on the gut microbiota through a bath or local antimicrobial therapies is limited. Results: The gut microbiota of gentamicin, recovered, and control Rana dybowskii groups were compared by Illumina high-throughput sequencing, and the functional profiles were analysed using a phylogenetic investigation of communities by the reconstruction of unobserved states (PICRUSt). Furthermore, the relationship between gut microbiota structures and forecast function compositions was determined. The results showed that the alpha diversity indices were significantly reduced by the gentamicin bath, which significantly changed the composition of the gut microbiota. After 7 days, the gut microbiota was still similar to that during the gentamicin bath. Forty-four indicator species were selected at the genus level, namely, 42 species indicating the control group and 2 species indicating the gentamicin and recovery groups. Potential pathogenic bacteria belonging to Aeromonas, Citrobacter, and Chryseobacterium significantly decreased after the gentamicin bath. The community similarity assays did not show an obvious discrepancy in the functional composition between the gentamicin and control frogs, indicating that the functions of the gut bacterial community were highly redundant. Conclusions: The gentamicin bath significantly reduced the alpha diversity of the gut microbiota of R. dybowskii. Gentamicin significantly changed the structure of the gut microbiota, and the gut microbiotas exhibited weak resilience and did not totally recover after seven days. The gentamicin bath did not change the functional composition of the gut microbiota of R. dybowskii, and there was no significant correlation between the composition of the gut microbiota and the functional composition, illustrating the high intestinal functional redundancy of the frog gut bacterial community. This work offers basic data for upcoming research, including the establishment of the amphibian gut microbiota and local antibiotic administration, and has important implications for aquaculture management and amphibian conservation.

show abstract

“…These data suggest that the recovery of gut microbiota likely depends on type of antibiotics, drug dose regimen, and host. Similarly, other factors may affect the recovery such as host diet, community context, environmental reservoirs, post-antibiotic environment factors like probiotic intake and autologous FMT (Ng et al, 2019; Suez et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Dynamic modulation of spleen germinal center reactions by gut bacteria during Plasmodium infection

Mandal

Denny

Namazzi

et al. 2021

Preprint

View full text Add to dashboard Cite

Gut microbiota educate the local and distal immune system in early life to imprint long-term immunological outcomes while maintaining the capacity to dynamically modulate the local mucosal immune system throughout life. It is unknown if gut microbiota provide signals that dynamically regulate distal immune responses following an extra-gastrointestinal infection. Using the murine model of malaria, we show that existing spleen germinal center reactions are malleable to dynamic cues provided by gut bacteria that impact parasite burden. Gut bacteria composition was also shown to correlate with the severity of malaria in humans. Whereas antibiotic-induced changes in gut bacteria has been associated with immunopathology or impairment of immunity, our data demonstrate antibiotic-induced changes in gut bacteria can enhance humoral immunity to Plasmodium. This effect is not universal, but depends on baseline gut bacteria composition. These data demonstrate the dynamic communications that exist between gut bacteria and the systemic immune system as well as the plasticity of an ongoing humoral immune response.

show abstract

Recovery of the Gut Microbiota after Antibiotics Depends on Host Diet, Community Context, and Environmental Reservoirs

Cited by 48 publications

References 0 publications

Establishment and Resilience of Transplanted Gut Microbiota in Aged Mice

Establishment and Resilience of Transplanted Gut Microbiota in Aged Mice

Changes in the Gut Microbiota Diversity of Brown Frogs (Rana Dybowskii) After Local Antimicrobial Therapy

Dynamic modulation of spleen germinal center reactions by gut bacteria during Plasmodium infection

Contact Info

Product

Resources

About