Recovery of structurally intact norovirus from food-contact surfaces

Ha, Ji‐Hyoung; Kim, Myung L.; Choi, Changsun; Choi, In‐Soo; Myoung, Jinjong; Ha, Sang‐Do

doi:10.1016/j.foodcont.2014.07.044

Cited by 12 publications

(6 citation statements)

References 35 publications

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“…These results did not show contamination; consequently, it demonstrated the specificity of the technique as nonspecific binding was not found. Our results are corroborated by other studies that have also demonstrated important findings on the capacity of IMS to concentrate viruses and on the increase of sensibility when combined with a molecular approach such as PCR or qPCR [29,31,41,42]. A result that must be noted is the increase of efficiency in detecting samples with low amounts of HAdV genomic copies when these were concentrated by IMS.…”

Section: Standardization Of Ims Parameterssupporting

confidence: 89%

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

et al. 2021

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The main goal of this study was to apply magnetic bead surface functionalization in the form of immunomagnetic separation (IMS) combined with real-time polymerase chain reaction (qPCR) (IMS-qPCR) to detect Human mastadenovirus species C (HAdV-C) and F (HAdV-F) in water samples. The technique efficiency was compared to a nonfunctionalized method (ultracentrifugation) followed by laboratory detection. Tests were carried out to standardize IMS parameters followed by tests on 15 water samples concentrated by IMS and ultracentrifugation. Microscopic analyses detected a successful beads–antibody attachment. HAdV was detected up to dilutions of 10−6 by IMS-qPCR, and samples concentrated by IMS were able to infect cell cultures. In water samples, HAdV-C was detected in 60% (monoclonal) and 47% (polyclonal) by IMS-qPCR, while 13% of samples concentrated by ultracentrifugation gave a positive result. HAdV-F was positive in 27% of samples by IMS-qPCR (polyclonal) and ultracentrifugation and 20% by IMS-qPCR (monoclonal). The rate of detection varied from 4.55 × 102 to 5.83 × 106 genomic copies/L for IMS-qPCR and from 2.00 × 102 to 2.11 × 103 GC/L for ultracentrifugation. IMS showed to be a more effective concentration technique for HAdV than ultracentrifugation, improving the assessment of infectious HAdV in water resources.

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Section: Standardization Of Ims Parameterssupporting

confidence: 89%

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

et al. 2021

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“…Moreover, ( Bhardwaj J. et al, 2021 ) demonstrate that non-porous fomites are the main contributors to the transmission of viral infection because the survival duration of infectious viruses on them is much longer than that on porous fomites. To investigate the survivability of HuNoV, Ha et al (2015) report that HuNoV GII.4 exists on artificially contaminated stainless-steel surfaces for up to 4 weeks following artificial inoculation. Previous studies have already investigated the use of UVC light to disinfect noroviruses ( Jean et al, 2011 ; Bosshard et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Virucidal Efficacy of Human Norovirus Using Combined Sprayed Slightly Acidic Electrolyzed Water and Ultraviolet C-Light-Emitting Diode Irradiation Treatment Based on Optimized Capture Assay for Quantitative RT-qPCR

Song¹,

Dang²,

Ha³

et al. 2022

Front. Microbiol.

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Slightly acidic electrolyzed water (SAEW), an effective non-thermal virucidal treatment, is used widely to prevent infectious viral cross-contamination. Surface disinfection technologies using ultraviolet C-light-emitting diode (UVC-LED) irradiation have recently attracted considerable attention. The SAEW sprayer technique is an efficient approach to preventing the spread of infectious viral pathogens in the public healthcare sector. Therefore, we investigated a small-scale system comprising sprayed SAEW disinfection combined with UVC-LED irradiation to inactivate the human norovirus (HuNoV) in the environment. A stainless-steel surface was inoculated with a HuNoV genogroup II genotype 4 (GII.4) to achieve maximum reduction values of 3.21 log10 genomic copies. For optimal disinfection conditions, the response surface methodology based on the Box–Behnken design revealed that the specific treatment conditions for inactivation of HuNoV GII.4 were an SAEW droplet volume of 180 μL, 30 ppm available chlorine concentration of SAEW, and a UVC-LED exposure dose of 2 mJ/cm2. The results indicate that the combined disinfection treatment could efficiently prevent the spread of HuNoVs in environment. Furthermore, the quadratic polynomial equations of the 3-D response surface can be employed to predict the effects of combined disinfection treatment on HuNoV contamination on environmental surfaces. Therefore, sprayed SAEW disinfection combined with UVC-LED irradiation proposed in this study may offer insights for designing optimal control strategies and techniques to prevent the transmission of infectious diseases, particularly HuNoV.

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“… 12 Scientists in the Netherlands have announced that the RNA virus was found in the WWTPs in the city of Amersfoort, 32 miles south‐east of Amsterdam. 13 For SARS‐COV‐2 RNA was found in sewerage across 19 Australian locations, according to a study variable affected individuals ranged from 171 to 1090. individuals were found within neighborhoods. 14 From this corresponding report, they additionally reported because viral RNA transcripts existed spelled out utilizing this qualitative polymerase chain reaction reverse transcriptase, culminating were two instances of confirmed identification from the WWTPs inside one six‐day period duration.…”

Section: Initial Covid‐19 Conditions In Wastewatermentioning

confidence: 99%

“…12 Scientists in the Netherlands have announced that the RNA virus was found in the WWTPs in the city of Amersfoort, 32 miles south-east of Amsterdam. 13…”

Section: Initial Covid-19 Conditions In Wastewatermentioning

confidence: 99%

Consequence of COVID‐19 occurrences in wastewater with promising recognition and healing technologies: A review

Manimekalai

Arulmozhi

Ammasi

et al. 2022

Env Prog and Sustain Energy

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Presently, the coronavirus (COVID‐19) epidemic presents a major threat to global communal fitness also socio‐financial development. Ignoring worldwide isolation as well as shutdown attempts, the occurrence of COVID‐19 infected patients continues to be extremely large. Nonetheless, COVID‐19's final course, combined with the prevalence of emerging contaminants (antibiotics, pharmaceuticals, nanoplastics, pesticides, and so forth) in wastewater treatment plants (WWTPs), presents a major problem in wastewater situations. The research, therefore, intends near examine an interdisciplinary as well as technical greet to succor COVID‐19 with subsequent COVID cycles of an epidemic as a framework for wastewater treatment settings. This research investigated the potential for wastewater‐based epidemiology to detect SARS‐CoV‐2 also the enzymes happening in wastewater conditions. In addition, a chance for the incorporation into the WWTPs of emerging and robust technologies such as mesmeric nanobiotechnology, electrochemical oxidation, microscopy, and membrane processes to enhance the overall likelihood of environmental consequences of COVID‐19 also strengthen such quality of water is resolved.

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Recovery of structurally intact norovirus from food-contact surfaces

Cited by 12 publications

References 35 publications

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

Functionalized Surfaces as a Tool for Virus Sensing: A Demonstration of Human mastadenovirus Detection in Environmental Waters

Evaluation of Virucidal Efficacy of Human Norovirus Using Combined Sprayed Slightly Acidic Electrolyzed Water and Ultraviolet C-Light-Emitting Diode Irradiation Treatment Based on Optimized Capture Assay for Quantitative RT-qPCR

Consequence of COVID‐19 occurrences in wastewater with promising recognition and healing technologies: A review

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