Recovery of serum proteins using cellulosic affinity membrane modified by immobilization of Cu2+ ion

Abstract

SYNOPSISAn affinity membrane was prepared from a porous cellulose membrane, and adsorption and recovery of serum proteins were investigated from the viewpoint that affinity membranes are efficacious against separation and purification of biomaterials. Into the cellulose membrane, iminodiacetate (IDA) group that acts as a ligand to metal ions was introduced (Cell-IDA membrane), and then Cu2+ ion was immobilized (Cell-IDA-Cu membrane). Bovine serum albumin (BSA) and y-globulin (BrG), which are the major proteins in blood, were adopted as model proteins to be separated. The Cell-IDA-Cu membrane had large adsorption capacity for these proteins despite the low degree of modification. The amounts of proteins adsorbed on the Cell-IDA-Cu membrane increased with increasing pH, and ByG was adsorbed more than BSA. High protein recoveries from the Cell-IDA-Cu membrane were obtained. The separation of these proteins was also conducted under the optimum conditions of adsorption and recovery, and ByG was concentrated more than BSA although the initial concentration of ByG was lower than that of BSA. 0 1996 John Wiley & Sons, Inc. I NTRO DUCT10 NA number of studies on the separation and purification of biomaterials, such as proteins and enzymes, have been developed with a growing demand for them in many fields ranging from food to drug. Affinity column chromatography is one of the most powerful techniques to separate and purify them, and different kinds of packings are available. Immobilized metal affinity chromatography (IMAC) was also evolved for the isolation of biomaterials.' There have been extensive discussions on the properties as well as preparation of IMAC.2-6 In IMAC, transition metal ions immobilized on polymer matrices are coordinated by proteins through their electron donor groups, such as histidine, cysteine, and tryptophanIn addition to the development of novel adsorbents for affinity columns, improvements in adsorbent geometry have been undertaken to process a large quantity of proteins rapidly. In order to obtain the higher performance on the processing ability of

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Preparation and performance of cellulose acetate/polyethyleneimine blend microfiltration membranes and their applications

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Membrane Chromatography: Preparation and Applications to Protein Separation

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