Recovery of regenerating goldfish retinal ganglion cells is slowed in the absence of the topographically correct synaptic target

Burmeister, Donald W.; Dunn-Meynell, Ambrose A.

doi:10.1016/0006-8993(87)90824-9

Cited by 4 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As mentioned, cell death in the retina depends on the type of optic nerve lesion, as occurs in Rana pipiens [23] or in the goldfish according to the number of lesions [53], in addition, topographical specificity also influences the recovery of cell body after damage in the goldfish [5]. Optic axotomy not only increased the number of neurites per cell and the branching, it also modified the effect of taurine.…”

Section: Discussionmentioning

confidence: 93%

“…Optic tectum was obtained without crush or after crush of the optic nerve and cultured for several days, the medium was filtered and added in 50% fresh medium. Length of neurites was measured in (A) intact retinal explants with medium from intact optic tectum, F (5,117) target in this process. In addition, anterograde transport of several molecules, including taurine [17,39], from the retina to the tectum also could regulate outgrowth.…”

Section: Discussionmentioning

confidence: 99%

“…After lesion of the optic nerve in the goldfish, the morphological changes of ganglion cells begin to disappear when axons start innervation of the tectum, however, it takes 3 months for these neurons to return to normal shape [32]. The tectum organizes the fibers coming from the retina, indicating topographical specificity by different elements in the target [5]. Contact with appropriate targets triggers intracellular signals before synapses are formed and the cells secrete transmitters to facilitate the proper innervation, the activitydependent refinement of connections, and the right communication, which results from retrograde and anterograde transport of messengers [18].…”

Section: Introductionmentioning

confidence: 99%

“…Medium from intact (Medium A) or post-crush (Medium B) optic tectum at 3, 6, 10, 14 or 20 days after crush were cultured for 3 days, the medium was filtered and added in 50% fresh medium. Length of neurites was measured in intact retinal explants, F(5,137) = 2.85, PB 0.02, and in postcrush retinal explants, F (6,337) = 8.54, PB 0.001. * P B 0.05 respecting control.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Differential taurine effect on outgrowth from goldfish retinal ganglion cells after optic crush or axotomy. Influence of the optic tectum

Cubillos¹,

Urbina²,

Lima³

2000

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

The interaction between innervated tissues, targets and nerves is crucial in the maintenance of physiological conditions, and the disturbance of this harmony causes the production of morphological and biochemical changes. After lesion of the optic nerve, several modifications take place in the retina, the optic tectum and the optic nerve. The influence of the tectum on the outgrowth from the goldfish retina and the possible role of taurine was studied. Ganglion retinal cells were identified by retrolabeling with Dil. Crushing the optic nerve 10 days prior to plating retinal cells, as compared with optic axotomy, did not affect the survival of cultured retinal cells, as well as the length of the neurites. However, the number of neurites per cell and the branching of the longest fiber were higher after axotomy than after crushing. The addition of taurine to the medium did not modify this response at 5 days in culture. At early periods in culture, the stimulatory effect on isolated ganglion cell outgrowth produced by taurine was enhanced after axotomy respecting crushing of the optic nerve, but was not affected in retinal explants. The addition of medium from cultured optic tectum several days post‐crush of the optic nerve to retinal explants from intact retinas or coming from post‐crush retina modified the outgrowth, being inhibitory or stimulatory in a time‐dependent manner. The co‐culture of optic tectum and retina also affected the outgrowth from the retina with a byphasic shape. The results support the differential response of the retina facing partial or complete interruption with the target and limit the effect of taurine to early periods in culture. In addition, the production of inhibitory factors from the tectum, plus the stimulatory ones, are strongly supported by this work.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Differential taurine effect on outgrowth from goldfish retinal ganglion cells after optic crush or axotomy. Influence of the optic tectum

Cubillos¹,

Urbina²,

Lima³

2000

Intl J of Devlp Neuroscience

View full text Add to dashboard Cite

show abstract

“…Axons from temporal RGCs (projecting to intact tectum) are represented earlier, and recover from chromatolysis faster, than those arising from nasal RGCs (now deprived of their normal tectal region) (Schmidt, 1983;Burmeister and Dunn-Meynell, 1987). A complementary finding is that, following caudal collicular ablation in neonatal hamster, compression of the projection is more accurate for temporal than for nasal RGC axons (Finlay et al, 1979).…”

Section: Restoration Of Topographymentioning

confidence: 93%

Regenerating optic axons restore topography after incomplete optic nerve injury

Dunlop

Tee

Goossens

et al. 2007

J of Comparative Neurology

View full text Add to dashboard Cite

Following complete optic nerve injury in a lizard, Ctenophorus ornatus, retinal ganglion cell (RGC) axons regenerate but fail to restore retinotectal topography unless animals are trained on a visual task (Beazley et al. [ 1997] J Comp Neurol 370:105-120, [2003] J Neurotrauma 20:1263-1270). Here we show that incomplete injury, which leaves some RGC axons intact, restores normal topography. Strict RGC axon topography allowed us to preserve RGC axons on one side of the nerve (projecting to medial tectum) while lesioning those on the other side (projecting to lateral tectum). Topography and response properties for both RGC axon populations were assessed electrophysiologically. The majority of intact RGC axons retained appropriate topography in medial tectum and had normal, consistently brisk, reliable responses. Regenerate RGC axons fell into two classes: those that projected topographically to lateral tectum with responses that tended to habituate and those that lacked topography, responded weakly, and habituated rapidly. Axon tracing by localized retinal application of carbocyanine dyes supported the electrophysiological data. RGC soma counts were normal in both intact and axotomized RGC populations, contrasting with the 30% RGC loss after complete injury. Unlike incomplete optic nerve injury in mammals, where RGC axon regeneration fails and secondary cell death removes many intact RGC somata, lizards experience a "win-win" situation: intact RGC axons favorably influence the functional outcome for regenerating ones and RGCs do not succumb to either primary or secondary cell death.

show abstract

Effect of conditioning lesions on regeneration of goldfish optic axons: time course of the cell body reaction to axotomy

et al. 1990

View full text Add to dashboard Cite

Recovery of regenerating goldfish retinal ganglion cells is slowed in the absence of the topographically correct synaptic target

Cited by 4 publications

References 19 publications

Differential taurine effect on outgrowth from goldfish retinal ganglion cells after optic crush or axotomy. Influence of the optic tectum

Differential taurine effect on outgrowth from goldfish retinal ganglion cells after optic crush or axotomy. Influence of the optic tectum

Regenerating optic axons restore topography after incomplete optic nerve injury

Effect of conditioning lesions on regeneration of goldfish optic axons: time course of the cell body reaction to axotomy

Contact Info

Product

Resources

About