Recovery of proteins and other biological compounds using fibrous materials: I. Adsorption by salt addition

Chen, Li Ang; Carbonell, Ruben G.; Serad, George A.

doi:10.1002/(sici)1097-4660(199908)74:8<733::aid-jctb111>3.3.co;2-w

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“…In a previous study, CAF were shown to act as a surface adsorbant not simply as a ®lter aid of salted-out proteins. 19 In the present system¯ocs formed by BSA±CMC did not adsorb to the CAF.…”

Section: Bsa Recovery From Solution Using Cmc and Cmc Cafmentioning

confidence: 49%

“…This is almost two orders of magnitude lower than the dosages required when salt was used as the driving force for the adsorption of particles and biomolecules on the ®brets (around 46 g per g protein). 19 It is also observed that there is an optimal dosage of CMC required in the process. Addition of polyelectrolyte beyond the optimum level resulted in lower protein removal, as previously observed with a model protein system (Fig 1), and higher turbidity of the ®nal ®ltrate (Fig 5(b)).…”

Section: Optimization Of the Cmc Ctf/caf Systemmentioning

confidence: 99%

“…27 Dissolved proteins may not adsorb to the surface of the ®brets unless a stronger driving force is provided, such as addition of salts. 19 When CTF and CMC were used together, the ®nal protein concentration in the ®ltrate from the solution of BSA fats was slightly lower than from the BSA only. However, the improvement on the overall BSA removal from solution, as compared with the system using CMC alone, was not as signi®cant as in the case of the actual wastewater samples.…”

Section: Effect Of Fats On Protein Recovery By Cmc Ctf/ Caf ± Model Pmentioning

confidence: 99%

“…In a previous work, we studied the effect of salt addition. 19 Cellulose triacetate ®brets (CTF) were found to be the most appropriate material, having optimal degrees of hydrophilicity for dispersion in aqueous environmens, and hydrophobicity for interaction with salted-out proteins. CTF also have a high surface area for adsorption to take place and a highly ®brillated structure for the retention of small aggregates by physical entrapment.…”

Section: Introductionmentioning

confidence: 99%

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Recovery of proteins and other biological compounds using fibrous materials: II. Flocculation by polyelectrolyte addition

Chen

Carbonell

Serad

1999

J. Chem. Technol. Biotechnol.

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Polyelectrolytes have been used in wastewater treatment processes to destabilize colloidal suspensions of proteins, cells and other biological compounds, resulting in¯occulation. When a solution containing a single model protein, bovine serum albumin (BSA), is treated with a polyelectrolyte, carboxymethyl cellulose (CMC), large and strong¯ocs are formed, which are easily retained by a 20 mm pore size ®lter. However, when a mixture of proteins, cells, and fats from an actual wastewater sample is treated in the same manner, smaller and weaker¯ocs are observed. An adsorption and ®ltration process for the recovery of valuable biological compounds using cellulosebased ®brous materials has been developed. When used simultaneously with CMC, cellulose acetate and triacetate ®brets (CAF and CTF) resulted in high recovery of biomolecules from solution at very low dosages of both polyelectrolyte and ®brets. CMC interacts with biomolecules by electrostatic interactions and polymer bridging, while CTF/CAF facilitate¯oc growth by adsorption and bridging of primary particles and by entrapment of small aggregates within their highly ®brillated microstructure.

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Section: Bsa Recovery From Solution Using Cmc and Cmc Cafmentioning

confidence: 49%

Section: Optimization Of the Cmc Ctf/caf Systemmentioning

confidence: 99%