Recovery of Pantoea ananatis, causal agent of center rot of onion, from weeds and crops in Georgia, USA

Gitaitis, R. D.; Walcott, R. R.; Culpepper, Stanley; Sanders, H.; Zolobowska, L.; Langston, David B.

doi:10.1016/s0261-2194(02)00078-9

Cited by 68 publications

(75 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4c and d). The representative sequence of OTU GP4 was identical to that of P. ananatis, which is a plant-associated bacterium isolated from diverse plants, including soybeans (14). The representative sequence of OTU FM1 had a high level of identity to that of E. mundtii (99%), which has also been isolated from soybeans as a biopreserver producing bacteriocin (48), suggesting that the phytosphere could be a good source of beneficial bacteria for the fermentation industry.…”

Section: Discussionmentioning

confidence: 96%

Autoregulation of Nodulation Interferes with Impacts of Nitrogen Fertilization Levels on the Leaf-Associated Bacterial Community in Soybeans

Ikeda

Anda

Inaba

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

The diversities leaf-associated bacteria on nonnodulated (Nod ؊ ؉؉ soybeans (46% to 76%) and, conversely, increased those of Gammaproteobacteria and Firmicutes in these mutant soybeans. In the Alphaproteobacteria, cluster analyses identified two operational taxonomic units (OTUs) (Aurantimonas sp. and Methylobacterium sp.) that were especially sensitive to nodulation phenotypes under SN fertilization and to nitrogen fertilization levels. Arbuscular mycorrhizal infection was not observed on the root tissues examined, presumably due to the rotation of paddy and upland fields. These results suggest that a subpopulation of leaf-associated bacteria in wild-type Nod ؉ soybeans is controlled in similar ways through the systemic regulation of autoregulation of nodulation, which interferes with the impacts of N levels on the bacterial community of soybean leaves.)Although diverse microorganisms reside in the phytosphere as endophytes, epiphytes, and rhizosphere bacteria, many questions about the driving forces and ecological rules underlying the relationships between these microbes and plants remain unanswered (18,39). During their evolution, legumes have developed two systems for attaining mutual symbioses with rhizobia and mycorrhizae. One of the systems genetically required for rhizobial and arbuscular mycorrhizal interactions in plants overlaps in a common signaling pathway (CSP) leading to successful symbioses (24). Plants also have a control system for regulating the degree of nodulation and mycorrhization on roots by rhizobia and mycorrhizae, respectively. This autoregulatory system occurs through long-distance signaling between shoots and roots (33). Leguminous plants deficient in the CSP and autoregulation systems develop nonnodulated (Nod Ϫ ) and hypernodulated (Nod ϩϩ ) roots, respectively. However, the degree to which plants use similar or identical systems, such as CSP and autoregulation, for interactions with other microorganisms in the phytosphere remains unclear.Recently, it was shown that the bacterial and fungal community structures in the roots of symbiosis-defective mutants of Medicago truncatula (32) and soybean (22) differ from those in the roots of wild-type host plants; it was also shown that certain microbes preferentially associate with arbuscular mycorrhizal roots (41) and nodulated (Nod ϩ ) roots (22). However, unexpectedly, analyses of the rhizosphere community in soybeans have revealed that the bacterial community in nonnodulated soybeans is more similar to that in hypernodulated soybeans than to that in wild-type soybeans (22).The autoregulation of nodulation occurs through long-distance signaling between shoots and roots (33), and a heavy supply of nitrate to the roots of leguminous plants inhibits nodulation through autoregulation (6, 34). Thus, it is possible that the nodulation phenotype and host nitrogen status affect the structure of the microbial community in aboveground tissues, such as stems and leaves, as has been observed in roots (22). Indeed, the results of our previous study o...

show abstract

Section: Discussionmentioning

confidence: 96%

Autoregulation of Nodulation Interferes with Impacts of Nitrogen Fertilization Levels on the Leaf-Associated Bacterial Community in Soybeans

Ikeda

Anda

Inaba

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…to the aviation fuel environment is not surprising. Pantoea ananatis, the bacterium responsible for onion center rot [16], has not been previously documented as associated with hydrocarbons or hydrocarbon degradation. P. ananatis was isolated from the refueling truck and two storage tanks at Edwards AFB, CA.…”

Section: Discussionmentioning

confidence: 99%

Characterization of microbial contamination in United States Air Force aviation fuel tanks

Rauch

Graef

Rozenzhak

et al. 2005

J IND MICROBIOL BIOTECHNOL

106

View full text Add to dashboard Cite

Bacteria and fungi, isolated from United States Air Force (USAF) aviation fuel samples, were identified by gas chromatograph fatty acid methyl ester (GC-FAME) profiling and 16S or 18S rRNA gene sequencing. Thirty-six samples from 11 geographically separated USAF bases were collected. At each base, an above-ground storage tank, a refueling truck, and an aircraft wing tank were sampled at the lowest sample point, or sump, to investigate microbial diversity and dispersion within the fuel distribution chain. Twelve genera, including four Bacillus species and two Staphylococcus species, were isolated and identified. Bacillus licheniformis, the most prevalent organism isolated, was found at seven of the 11 bases. Of the organisms identified, Bacillus sp., Micrococcus luteus, Sphinogmonas sp., Staphylococcus sp., and the fungus Aureobasidium pullulans have previously been isolated from aviation fuel samples. The bacteria Pantoea ananatis, Arthrobacter sp., Alcaligenes sp., Kocuria rhizophilia, Leucobacter komagatae, Dietza sp., and the fungus Discophaerina fagi have not been previously reported in USAF aviation fuel. Only at two bases were the same organisms isolated from all three sample points in the fuel supply distribution chain. Isolation of previously undocumented organisms suggests either, changes in aviation fuel microbial community in response to changes in aviation fuel composition, additives and biocide use, or simply, improvements in isolation and identification techniques.

show abstract

“…Mattick 2008), we tested three P. ananatis-specific primer pairs in one assay: (1) PanITS1/EC5 (Walcott et al 2003), (2) PanITS1/EC5-Gi (Gitaitis et al 2002) and (3) Pan16S/ Pan16AS (De Boer 2003), using the strains hybridized to the microarray: M241, M304, M408, M471 and ATCC 33244. The sensitivity of the PCR detection was tested for each primer pair and bacterial strain on a series of a tenfold dilution of genomic DNA, starting with 50 ng/ll.…”

Section: Using a Touch-down Pcr Technique (Td-pcr) (Korbie Andmentioning

confidence: 99%

DNA microarray-based detection and identification of bacterial and viral pathogens of maize

et al. 2017

View full text Add to dashboard Cite

We describe the construction and evaluation of DNA microarray for simultaneous detection and identification of five microbial pathogens of maize: Pantoea ananatis, P. agglomerans, Enterobacter cloaceae subsp. dissolvens, Maize dwarf mosaic virus (MDMV) and Sugarcane mosaic virus (SCMV). Unlike other DNA microarrays described, our microarray comprises probes targeting the whole genomes of the tested pathogens. Control probes are complementary to genomes of closely related microorganisms that are nonpathogenic to maize and against maize and human genome sequences in order to avoid the potential false-positive results. Obtained results indicate that the fluorescence signals from pathogen and control probes are well distinguished in all performed experiments. The microarray's performance was compared with classical PCR-based pathogen detection method, and the versatility of the assay was tested in silico.

show abstract

Recovery of Pantoea ananatis, causal agent of center rot of onion, from weeds and crops in Georgia, USA

Cited by 68 publications

References 13 publications

Autoregulation of Nodulation Interferes with Impacts of Nitrogen Fertilization Levels on the Leaf-Associated Bacterial Community in Soybeans

Autoregulation of Nodulation Interferes with Impacts of Nitrogen Fertilization Levels on the Leaf-Associated Bacterial Community in Soybeans

Characterization of microbial contamination in United States Air Force aviation fuel tanks

DNA microarray-based detection and identification of bacterial and viral pathogens of maize

Contact Info

Product

Resources

About