Recovery of Oesophagotomum dentatum from pigs by isolation of parasites migrating from large intestinal contents embedded in agar-gel

Slotved, Hans Christian; Barnes, Elizabeth H; Bjørn, H.; Çhristensen, C. M.; Eriksen, L.; Roepstorff, Allan; Nansen, P.

doi:10.1016/0304-4017(95)00916-7

Cited by 81 publications

(39 citation statements)

References 7 publications

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“…Helminth-free pigs (Danish Landrace × Yorkshire × Duroc; both sexes; 20 kg live weight) were inoculated by intragastric intubation with 6×10 3 L 3 . Fourth larval (L 4 ) and adult stages of O. dentatum were recovered from large intestinal content on day 10 (early L 4 ), day 15 (late L 4 ) or day 25 (adult) after inoculation, respectively, harvested into physiological saline using an agar gel method (Slotved et al 1996) (with both sexes being separated using a dissection microscope) and subsequently snap frozen in liquid nitrogen. The specific identity of adult nematodes was determined using an existing description (Haupt 1966).…”

Section: Parasite Materialsmentioning

confidence: 99%

Ubiquitin-conjugating enzyme genes in Oesophagostomum dentatum

et al. 2006

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Full-length genes representing different isoforms of the ubiquitin-conjugating enzyme UBC-2 were isolated from Oesophagostomum dentatum, cloned and sequenced. The alignment of their sequences (designated Od-ubc-2.1 to Od-ubc-2.3) revealed nucleotide variation at three positions within the predicted open reading frame of 444 bp. Substitutions were at positions 141 (A<-->G), 142 (A<-->G) and 296 (T<-->C). Both former substitutions resulted in amino acid changes from a glycine residue to an arginine residue, whereas the latter resulted in a change from isoleucine to threonine. Comparison of predicted OD-UBC-2 with UBC-2 (protein) homologues/orthologues from 12 other species representing nematodes, Drosophila melanogaster, Saccharomyces cerevisiae, mice and humans revealed identities between species varying from 77 to 100% at the amino acid level, and motifs associated with protein conformation and function were identified. While the function of a representative ubc-2 gene from O. dentatum could not be established in C. elegans, it is likely to play a key role in the catabolism of proteins and in the development of O. dentatum.

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Section: Parasite Materialsmentioning

confidence: 99%

Ubiquitin-conjugating enzyme genes in Oesophagostomum dentatum

et al. 2006

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“…The rapid collection of fully mobile worms in a clean suspension of saline was made possible by a newly developed agar-gel migration technique for recovery of nematodes in the pig (Slotved et al 1996). Almost all worms emerge from the agar gel within approximately 1-1.5 h of the setup (unpublished observations).…”

Section: Transplantation Proceduresmentioning

confidence: 99%

“…The large intestines were divided into five sections [cecum (1), colon 0-20% (2), colon 21-40% (3), colon 41-60% (4), and colon 61-100% (5)] to study the localization of the worm population. Then, 10% of the contents from each section, dissolved in 0.9% saline solution, was mixed with equal amounts of 2% agar according to the method described by Slotved et al (1996). Worms collected using the agar-gel technique were counted and differentiated according to sex and stage, and the length of adult worms was measured using a digital image-analysis system.…”

Section: Procedures At Killingmentioning

confidence: 99%

Growth and fecundity of Oesophagostomum dentatum in high-level infections and after transplantation into naive pigs

Çhristensen¹,

Barnes²,

Nansen³

et al. 1996

Parasitology Research

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This experiment was designed to examine the growth, proportion of stages, and fecundity of an Oesophagostomum dentatum population by transplantation of a known small number of worms from a high-density population into helminth-naive recipient pigs. Approximately 1,500 4-week-old worms [69% fourth-stage larvae (L4), 31% adult worms] were transplanted into each of 5 recipient pigs (group B), and these pigs, along with a group of 5 high-level-infection control pigs (group C), were killed at 4 weeks after transplantation to determine and compare the worm burdens. By 2 weeks after transplantation and throughout the experiment, fecal egg counts of group B exceeded those of group C and the fecundity of the worms was higher, though not statistically significantly so, in the transplanted worms. In the recipient pigs, all worms (approx. 70% establishment) had developed to the adult stage and were significantly longer than worms recovered from the group C pigs.

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“…The mucosal surface was washed thoroughly with warm water (38°C). One liter aliquots were then processed for worm recovery and enumeration by a modification of the agar gel technique of Slotved et al (1996). A 2% agar solution (Bacto-Agar) was prepared by mixing the agar with tap water.…”

Section: Calculating the Reduction In Worm Burdenmentioning

confidence: 99%

Efficacy of ivermectin in calves against a resistant Cooperia oncophora field isolate

Njue

Prichard

2004

Parasitol Res

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Since 1999, two Cooperia oncophora isolates, originally obtained from the United Kingdom, have been maintained by regular passage through calves at the Macdonald Campus, McGill University farm. One isolate, IVS, was originally susceptible to ivermectin, while the IVR isolate was originally resistant to ivermectin. These two isolates have been used to study the mechanisms of ivermectin resistance. To confirm the susceptible/resistant status after 4 years of passaging through calves, a controlled study was performed in which two worm-free calves were experimentally infected with IVS and another two worm-free calves with the IVR infective larvae. The calves were treated with ivermectin (0.2 mg/kg) subcutaneously (Ivomec Injection) 21 days after infection. Ivermectin at the recommended dose was 100% effective at eliminating the IVS isolate, since no eggs were found in feces, and no adult worms were found in the small intestine of the treated IVS-infected calf. In contrast, the IVR-infected calf continued to pass eggs in feces even after treatment with ivermectin, and adult worms (250) were found in the small intestine at necropsy. The untreated calves had 1,330 and 848 adult worms, respectively, for the IVS and IVR infected animals.

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Recovery of Oesophagotomum dentatum from pigs by isolation of parasites migrating from large intestinal contents embedded in agar-gel

Cited by 81 publications

References 7 publications

Ubiquitin-conjugating enzyme genes in Oesophagostomum dentatum

Ubiquitin-conjugating enzyme genes in Oesophagostomum dentatum

Growth and fecundity of Oesophagostomum dentatum in high-level infections and after transplantation into naive pigs

Efficacy of ivermectin in calves against a resistant Cooperia oncophora field isolate

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