Recovery of nicotine-free proteins from tobacco leaves using phosphate buffer system under controlled conditions

Fu, Hao; Machado, Peter A.; Hahm, Tae‐Shik; Kratochvil, Robert J.; Wei, Cheng‐I; Lo, Y. Martin

doi:10.1016/j.biortech.2009.10.045

Cited by 32 publications

(23 citation statements)

References 28 publications

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“…A tobacco extract has been shown to induce apoptosis in oral squamous carcinoma cells [15]. Interestingly, an optimal phosphate buffer system can effectively remove nicotine residuals and recover proteins from tobacco leaves up to 94.5% [2]. In the current study, we proposed that the nicotine-free tobacco extract can intervene tumorigenesis by targeting the DNA damage response pathway.…”

Section: Introductionmentioning

confidence: 86%

“…The nicotine-free tobacco extract used in this study was prepared form low alkaloid tobacco (N. tabacum L. cv. MD-609LA) using the Na 2 HPO 4 -KH 2 PO 4 buffer system as described previously [2]. The nicotine was completely removed from the protein and undetectable in the extract [2].…”

Section: Cell Culture and Chemicalsmentioning

confidence: 99%

“…Due to health concerns and government regulations in the last 20 years, tobacco production has been declining. Irrespective of smoking uses, tobacco is in fact a good source of biomass with superior protein quality and nutrition value [1,2]. Tobacco contains thousands of chemicals and proteins with various characteristics [3].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Nicotine-free Tobacco Extract on DNA Damage Responses in Cancerous and Non-cancerous Cells

Lo¹

2014

J Carcinog Mutagen

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The use of cigarette products tags tobacco as a crop with serious health concerns, but nutraceutical potentials of tobacco constituents other than nicotine have not been adequately explored. The objective of this study was to investigate the effect of nicotine-free tobacco extract on the activation of tumorigenesis barriers. Four lines of human cells were treated with a nicotine-free tobacco extract (0.1 and 1 mg/mL, 0-48 h). MRC-5 and CCD841 non-cancerous cells treated with the tobacco extract showed hypersensitivity, apoptosis and cell cycle arrest in S and G 2 /M phases, and induction of DNA damage response as evidenced by phosphorylation of ataxia telangiectasia mutated at Ser-1981 and histone H2A.X at Ser-139. In contrast, HCT 116 cancerous cells, with or without functional DNA mismatch repair, were resistant to the tobacco extract treatment. These results suggest that tobacco components other than nicotine may have a chemoprevention potential that stifles tumorigenesis at the early stage of tumorigenesis.

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Section: Introductionmentioning

confidence: 86%

Section: Cell Culture and Chemicalsmentioning

confidence: 99%

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Effect of Nicotine-free Tobacco Extract on DNA Damage Responses in Cancerous and Non-cancerous Cells

Lo¹

2014

J Carcinog Mutagen

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“…However, there are cultivars available in tobacco that produce reduced levels of unwanted secondary metabolites like nicotine (Menassa et al 2001). Alternatively, efficient purification systems for the removal of unwanted nicotine could be utilized to get nicotine-free recombinant proteins (Fu et al 2010). Other leafy crops that have been used for recombinant protein production include spinach (Yusibov et al 2002), lettuce (Ruhlman et al 2007) and lucerne (Khoudi et al 1999).…”

Section: Strategies For Optimum Protein Productionmentioning

confidence: 99%

Molecular farming: strategies, expression systems and bio-safety considerations

Ahmad¹

2014

CZECH J GENET PLANT

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Ahmad K. (2014): Molecular farming: strategies, expression systems and bio-safety considerations. Czech J. Genet. Plant Breed., 50: 1-10.Molecular farming is an experimental application of biotechnology that involves the genetic modification of crops for the production of proteins and chemicals for medicinal and commercial purposes. The vast majority in the developing world cannot afford the high cost of therapeutics produced by existing methods. We need to produce not only new therapeutics but also cheaper versions of the existing ones. Molecular farming could offer a viable option for this growing need for biopharmaceuticals. Plant made therapeutics are cheaper, safer, can be abundantly produced and easily stored. Here, strategies and approaches utilized in plant molecular farming are discussed. Furthermore, the bio-safety considerations related to this emerging field are also discussed.

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“…Cigarette smoking involves inhaling the smoke regularly and habitually owing to the greatly addictive properties of nicotine (N. L. Benowitz, 1988;Aldo Leone, Landini, & Leone, 2010 Ludwig Reimann of Germany isolated nicotine from the tobacco plant in 1828 (Henningfield & Zeller, 2006). Nicotine is a hygroscopic oily liquid, and it is miscible with water in its base form, whereas its salt form is usually solid and water soluble to produce its nitrogenous base (Fu et al, 2010). Nicotine has two enantiomeric forms.…”

Section: Historical Backgroundmentioning

confidence: 99%

Development of Nicotine Loaded Chitosan Nanoparticles for Lung Delivery

Wang¹

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Cigarette smoking has become one of the leading causes of preventable deaths all over the world, causing a serious threat to human wellbeing and a tremendous economic burden to governments. The currently available treatment options for smoking cessation are not efficient. The pulmonary delivery of drugs by methods such as dry powder inhaler (DPI) has been recognized as one of the most efficient routes of drug delivery to the targeted area. The lung delivery of nicotine in this way would be expected to mimic the effects of tobacco smoking and could significantly reduce the negative health effects of smoking that result from nicotine addiction.The aim of this study is to develop nanoparticles with controlled physicochemical properties using biodegradable polymer of chitosan (CS) loaded with nicotine salt for pulmonary delivery as DPI formulations. The outcomes of this project are expected to be a safe and effective CS-based nicotine formulation for pulmonary delivery to treat nicotine dependence. This thesis specially addresses the research questions: (1) how to develop a feasible process to manufacture the nanoparticles suitable for pulmonary drug delivery using biodegradable polymer of CS containing nicotine salt; (2) how to develop an animal model for pulmonary drug delivery from DPI formulation using a nose-only inhalation device.The current therapeutic options for treatment of smoking addiction have been reviewed, and current advancements of technology in pulmonary drug delivery are summarised. Buccal administration of drugs exhibits a low oral bioavailability, and sublingual tablets and chewing gums can be swallowed before being absorbed.Although nasal spray of nicotine is a fast way to deliver nicotine into the bloodstream, Development of Nicotine Loaded Chitosan Nanoparticles for Lung Delivery iii the rate of absorption is still not as rapid as cigarette smoking. Therefore, it is proposed that delivery of nicotine with sustained drug release profile direct into the deep lung is likely to more effectively mimic the rapid effects of cigarette smoking on a physiological level, which could eliminate patient craving and allow the tapering of nicotine level over time to improve patients' compliance.Water in oil (w/o) emulsion crosslinking method has been used to fabricate nicotine hydrogen tartrate (NHT)-loaded CS nanoparticles which separate as solid microaggregates. The physicochemical properties of particles are characterized by a series of techniques. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) shows that the prepared particles are spherical in morphology with rough surface after loading CS particles with NHT. Dynamic Light Scattering (DLS)by Zetasizer determined nanoparticle size ranging from 167.6 nm to 411 nm, while DLS by Mastersizer demonstrated that the microaggregates of these nanoparticles are in the respirable range (<5µm). The surface positive charge as measured by zeta potential tends to decrease with increase of mass ratios of NHT to CS, which is in contr...

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Recovery of nicotine-free proteins from tobacco leaves using phosphate buffer system under controlled conditions

Cited by 32 publications

References 28 publications

Effect of Nicotine-free Tobacco Extract on DNA Damage Responses in Cancerous and Non-cancerous Cells

Effect of Nicotine-free Tobacco Extract on DNA Damage Responses in Cancerous and Non-cancerous Cells

Molecular farming: strategies, expression systems and bio-safety considerations

Development of Nicotine Loaded Chitosan Nanoparticles for Lung Delivery

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