Recovery of mitogenomes from whole genome sequences to infer maternal diversity in 1883 modern taurine and indicine cattle

Abstract

Maternal diversity based on a sub-region of mitochondrial genome or variants were commonly used to understand past demographic events in livestock. Additionally, there is growing evidence of direct association of mitochondrial genetic variants with a range of phenotypes. Therefore, this study used complete bovine mitogenomes from a large sequence database to explore the full spectrum of maternal diversity. Mitogenome diversity was evaluated among 1883 animals representing 156 globally important cattle breeds. Overall, the mitogenomes were diverse: presenting 11 major haplogroups, expanding to 1309 unique haplotypes, with nucleotide diversity 0.011 and haplotype diversity 0.999. A small proportion of African taurine (3.5%) and indicine (1.3%) haplogroups were found among the European taurine breeds and composites. The haplogrouping was largely consistent with the population structure derived from alternate clustering methods (e.g. PCA and hierarchical clustering). Further, we present evidence confirming a new indicine subgroup (I1a, 64 animals) mainly consisting of breeds originating from China and characterised by two private mutations within the I1 haplogroup. The total genetic variation was attributed mainly to within-breed variance (96.9%). The accuracy of the imputation of missing genotypes was high (99.8%) except for the relatively rare heteroplasmic genotypes, suggesting the potential for trait association studies within a breed.

Mitochondrial DNA diversity of D-loop region in three native Turkish cattle breeds

“…Indeed, AB is the most geographically distributed and preferred among native breeds, while ZAV is raised in the limited region of eastern Türkiye. As expected, nucleotide diversity was higher in native Turkish cattle breeds than in a previous study conducted on several cosmopolitan cattle breeds (ANG, BS, HF, JER and SIM) (Dorji et al, 2022). The long-term of artificial selection practices to increase phenotypic performance, such as milk yield, could lead to reduced genetic diversity and the extension of genomic homozygosity in pure cosmopolitan cattle breeds (Kelleher et al, 2017).…”

Genomic reconstruction of the successful establishment of a feralized bovine population on the subantarctic island of Amsterdam

“…Finally, mitochondrial haplogroups from each individual were inferred using MitoToolPy (Peng et al ., 2015) based on the “treeFile” provided by Dorji et al . (2022) for the ARS_UCD1.2 assembly.…”

“…Finally, mitochondrial haplogroups from each individual were inferred using MitoToolPy (Peng et al, 2015) based on the "treeFile" provided by Dorji et al (2022) for the ARS_UCD1.2 assembly. For each individual, a fasta file was obtained by extracting genotypes from the VCF file using the consensus command from bcftools v1.10.2 and using the reference genome fasta file.…”