Recovery of Microvascular Blood Perfusion and Energy Metabolism of the Obstructed Rabbit Urinary Bladder after Relieving Outlet Obstruction

Lin, Alex Tong‐Long; Chen, Kuang-Kuo; Yang, Chih-Hsin; Chang, Luke S.

doi:10.1159/000019780

Cited by 13 publications

(7 citation statements)

References 20 publications

(17 reference statements)

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“…However, whether a patient develops a higher post‐void residual or eventual urinary retention is not only dependent on the grade of BOO. Numerous in vitro and in vivo animal studies have reported the bladder's response to acute or chronic BOO . Several in vitro studies have shown a consistent relationship between increased bladder mass and altered contractile responses in muscle strips in prolonged BOO in rat, rabbit and cat preparations .…”

Section: What Is the Value Of Current Animal Models Of Concomitant DImentioning

confidence: 99%

Detrusor underactivity: Pathophysiological considerations, models and proposals for future research. ICI-RS 2013

Koeveringe

Rademakers

Birder

et al. 2014

Neurourol. Urodynam.

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Aims Detrusor underactivity, resulting in either prolonged or inefficient voiding, is a common clinical problem for which treatment options are currently limited. The aim of this report is to summarize current understanding of the clinical observation and its underlying pathophysiological entities. Methods This report results from presentations and subsequent discussion at the International Consultation on Incontinence Research Society (ICI-RS) in Bristol, 2013. Results and Conclusions The recommendations made by the ICI-RS panel include: Development of study tools based on a system’s pathophysiological approach, correlation of in vitro and in vivo data in experimental animals and humans, and development of more comprehensive translational animal models. In addition, there is a need for longitudinal patient data to define risk groups and for the development of screening tools. In the near-future these recommendations should lead to a better understanding of detrusor underactivity and its pathophysiological background.

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Section: What Is the Value Of Current Animal Models Of Concomitant DImentioning

confidence: 99%

Detrusor underactivity: Pathophysiological considerations, models and proposals for future research. ICI-RS 2013

Koeveringe

Rademakers

Birder

et al. 2014

Neurourol. Urodynam.

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“…Reperfusion after the cyclical periods of ischaemia/hypoxia would also be significantly greater in these pigs. Decreased blood flow (ischaemia) has also been shown in rat, rabbit and dog models of partial BOO [8–10]. In addition to partial BOO, acute overdistension (retention) has also been shown to result in ischaemia [11].…”

Section: Introductionmentioning

confidence: 99%

“…They all act by converting superoxide to peroxide which is then further broken down by catalase to oxygen and water. Because I/R is a major causal factor in the pathological response of the rabbit to partial BOO and other disorders [3–12], activation of SOD would be important to the recovery of the bladder. We have direct evidence (unpublished observations) that partial BOO results in the generation of free radical damage to detrusor smooth muscle proteins, which supports the importance of SOD within the bladder.…”

Section: Introductionmentioning

confidence: 99%

Effect of bladder ischaemia/reperfusion on superoxide dismutase activity and contraction

et al. 2005

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contractile studies. The contractile responses to field stimulation, carbachol (10 m mol/L), ATP and KCl were determined. The balance of the bladder body was separated into muscle and mucosa sections and analysed for SOD activity. RESULTSThere were few effects on contraction either directly after ischaemia or after 1 day of reperfusion. However, all contractile responses were significantly reduced at 7 and 14 days after ischaemia. SOD activity of the detrusor muscle was reduced significantly immediately after ischaemia and at 7 and 14 days of reperfusion. SOD activity of the mucosa was significantly greater than that of the muscle, and was significantly reduced by both ischaemia and all times of reperfusion. CONCLUSIONSThese studies show clearly that both ischaemia and reperfusion result in significantly lower activity of SOD, and in contractile dysfunctions, and that reperfusion results in greater decreases in both SOD activity and contractile responses than ischaemia alone.

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“…High pressure due to sustained overdistention can induce a reduction of blood flow to the bladder wall [ 14 ]. Additionally, pBOO increases the thickness of the bladder wall through hypertrophy of the detrusor smooth muscle and deposition of collagen tissues, resulting in a reduction of microvascular blood perfusion [ 15 ]. In addition, rapid reperfusion due to relief of pBOO in chronically ischemic bladders leads to the generation of free radicals and rapid oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

The preventive effect of a free radical scavenger on oxidative stress after the relief of partial bladder outlet obstruction in a rat model

Choo

Piao

2018

PLoS ONE

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AimsTo investigate the effect of a free radical scavenger (tempol) after relief of partial bladder outlet obstruction (pBOO) on bladder function in a rat model.MethodspBOO was induced in 50 eight-week-old female Sprague-Dawley rats and relieved 3 weeks later. The rats were divided randomly into 5 groups: sham-operated, tempol-treated for 1 week (Treat-1w) or 3 weeks (Treat-3w), and no treatment for 1 week (nonTreat-1w) or 3 weeks (nonTreat-3w). Awaken cystometrograms were obtained 1 or 3 weeks after relief according to the grouping. The bladders were isolated and weighed. H&E, Masson’s trichrome and TUNEL staining were used to analyze histological changes. The oxidative stress assessed using malondialdehyde. The expression of beta-3 adrenoreceptor was examined by Western blotting.ResultsThe tempol-treated groups exhibited a significant decrease in the number of non-voiding contractions per voiding cycle (nonTreat-1w vs. Treat-1w, 1.18±0.82 vs. 0.36±0.40, P = 0.010; nonTreat-3w vs. Treat-3w, 1.51±0.69 vs. 0.23±0.25, P = 0.002). The thickness and collagen fiber deposition of the detrusor muscle layer was significantly decreased in the treated groups. Apoptosis detected was mainly observed in the urothelial cell layer, although the rate of apoptosis was significantly decreased in the treated groups (48.9±3.36% vs. 32.7±11.10%, P = 0.024; 25.8±4.67% vs. 15.7±9.83%, P = 0.314). The tempol-treated groups showed significant decreases in the MDA concentrations at both 1 and 3 weeks after relief. The expression of the beta-3 adrenoreceptor was increased in the tempol-treated rats.ConclusionsIschemic reperfusion injury after relief of pBOO caused histological and functional changes in the bladder. Free radical scavenger treatment prevented this oxidative stress.

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Recovery of Microvascular Blood Perfusion and Energy Metabolism of the Obstructed Rabbit Urinary Bladder after Relieving Outlet Obstruction

Cited by 13 publications

References 20 publications

Detrusor underactivity: Pathophysiological considerations, models and proposals for future research. ICI-RS 2013

Detrusor underactivity: Pathophysiological considerations, models and proposals for future research. ICI-RS 2013

Effect of bladder ischaemia/reperfusion on superoxide dismutase activity and contraction

The preventive effect of a free radical scavenger on oxidative stress after the relief of partial bladder outlet obstruction in a rat model

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