Recovery of mare oocytes on a fixed biweekly schedule, and resulting blastocyst formation after intracytoplasmic sperm injection

Jacobson, Candace C.; Choi, Young-Ho; Hayden, Shelby S.; Hinrichs, Katrin

doi:10.1016/j.theriogenology.2010.01.013

Cited by 85 publications

(80 citation statements)

References 39 publications

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“…Then, transvaginal ultrasound-guided follicular puncture was developed in the mare [40,41]. Since then, the OPU technique has been extensively used in the equine [11][12][13][14][15][16][17][18][19][20][21]. The mean number of follicles punctured per mare per session ranges from two to 12 with an effect of mare [19], breed (lower number in pony mares compared with saddle mares) [12], the estrous cycle stage [14,15] and season [17].…”

Section: Discussionmentioning

confidence: 99%

“…Since then, the OPU technique has been extensively used in the equine [11][12][13][14][15][16][17][18][19][20][21]. The mean number of follicles punctured per mare per session ranges from two to 12 with an effect of mare [19], breed (lower number in pony mares compared with saddle mares) [12], the estrous cycle stage [14,15] and season [17]. The mean number of immature oocytes recovered per mare per aspiration session ranges from zero to five and the oocyte recovery rate per immature follicle in the mare ranges from 20% to 56% [12][13][14][15][17][18][19].…”

Section: Discussionmentioning

confidence: 99%

“…The mean number of follicles punctured per mare per session ranges from two to 12 with an effect of mare [19], breed (lower number in pony mares compared with saddle mares) [12], the estrous cycle stage [14,15] and season [17]. The mean number of immature oocytes recovered per mare per aspiration session ranges from zero to five and the oocyte recovery rate per immature follicle in the mare ranges from 20% to 56% [12][13][14][15][17][18][19]. In our study, the mean number of immature oocytes recovered from jennies ranged from 2.75 at the first session to a maximum of six at the forth session.…”

Section: Discussionmentioning

confidence: 99%

“…The first step is the collection of several immature oocytes on a donor female using ovum pick up (OPU). The OPU technique has been widely used in the equine [11][12][13][14][15][16][17][18][19][20][21]. However, to our knowledge, OPU has never been described in jennies.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

et al. 2016

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“…Normally only one pre-ovulatory follicle is present in each cycle during the breeding season, thus making the yield from this technique less effective than in cattle. However, the yield of useable oocytes can be increased by aspiration from all follicles greater than 1 mm in diameter [26,29].…”

Section: Embryo Technologiesmentioning

confidence: 99%

Animal Reproduction Technologies—Future Perspectives

Morrell¹,

Humblot²

2016

JAST-A

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Abstract:Reproductive biotechnologies, such as artificial insemination, sperm selection and embryo technologies, offer possibilities for animal producers to increase reproductive efficiency. There have been many significant developments in reproductive biotechnologies over the last few decades, e.g., in sperm handling and preservation, in vitro embryo production and preservation, sexing and cloning. This review discusses some of the key changes that have occurred and explores their potential for increasing the reproductive efficiency of domestic animals in the future. As a consequence, they also offer opportunities to facilitate or accelerate genetic selection. If properly used, they may contribute to increase the sustainability of animal production. The role of epigenetics in influencing phenotype is also considered.

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The equine oocyte: Factors affecting meiotic and developmental competence

Hinrichs

2010

Molecular Reproduction Devel

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SUMMARYThere is currently much interest in assisted reproduction techniques in the horse, however, many aspects of oocyte maturation, fertilization, and embryo development in the horse differ from those in other species. Because of the close attachment of the equine oocyte to the follicle wall, scraping of the follicle is the most effective method for oocyte recovery. A notable feature of equine oocytes is that those with expanded cumuli (Ex oocytes), which originate from atretic follicles, have higher meiotic competence (ability to mature to metaphase II in vitro) than do oocytes with compact cumuli (Cp oocytes). Cp oocytes originate in viable follicles but are largely juvenile. Recovery and culture of equine oocytes immediately after slaughter yields a higher maturation rate than that obtained from oocytes after ovary storage; this is related to damage to chromatin in Cp oocytes during storage. In contrast, developmental competence (rate of blastocyst development in vitro) is higher in oocytes recovered from the ovary after a delay. The optimum duration of maturation varies based on cumulus morphology and time of recovery from the ovary, but there is no difference in developmental competence between Ex and Cp oocytes. Because standard in vitro fertilization is not repeatable in the horse, oocyte transfer (surgical transfer of oocytes to the oviducts of inseminated mares) has been developed to allow fertilization of isolated oocytes. Fertilization in vitro may be achieved using intracytoplasmic sperm injection; culture of injected oocytes in a medium with high glucose can yield over 30% blastocyst development.Mol. Reprod. Dev. 77: 651-661,

show abstract

Recovery of mare oocytes on a fixed biweekly schedule, and resulting blastocyst formation after intracytoplasmic sperm injection

Cited by 85 publications

References 39 publications

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

Animal Reproduction Technologies—Future Perspectives

The equine oocyte: Factors affecting meiotic and developmental competence

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