Recovery of low volumes of wear debris from rat stifle joint tissues using a novel particle isolation method

Patel, Jay; Lal, Saurabh; Nuss, Katja; Wilshaw, Stacy‐Paul; Rechenberg, Brigitte von; Hall, Richard M.; Tipper, Joanne L.

doi:10.1016/j.actbio.2018.02.030

Cited by 8 publications

(13 citation statements)

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“… The data provide evidence that the method presented in Ref. [1] does not have a measureable effect on the size or morphology of particles. …”

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confidence: 59%

“…The rest of the protocol was performed as detailed in Ref. [1] . The experiment was repeated in three replica 0.25 g tissue samples doped with a volume of 1 mL of a 25 µm 3 mL −1 CoCrMo particle suspension, and in three replica 0.25 g tissue samples doped with a volume of 1 mL of a 25 µm 3 mL −1 Ti-6Al-4V particle suspension.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“… The data give an indication for the sensitivity (minimum amount of particles) that has been achieved using the particle isolation method presented in Ref. [1] . The data provide evidence that the method presented in Ref.…”

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confidence: 96%

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Validation of a novel particle isolation procedure using particle doped tissue samples

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A novel particle isolation method for tissue samples was developed and tested using particle-doped peri-articular tissues from ovine cadavers. This enabled sensitivity of the isolation technique to be established by doping tissue samples of 0.25 g with very low particle volumes of 2.5 µm3 per sample. Image analysis was used to verify that the method caused no changes to particle size or morphologies.

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“… The data provide evidence that the method presented in Ref. [1] does not have a measureable effect on the size or morphology of particles. …”

mentioning

confidence: 59%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

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Validation of a novel particle isolation procedure using particle doped tissue samples

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“…Recovery as a weight % was calculated. Filtered particles were inspected by scanning electron microscopy and elemental analysis as detailed in [2] to ensure that the recovered mass was not due to contamination.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…Details of the commercial silicon nitride nanoparticles used in this study, including the results of scanning electron microscopy, elemental analysis and particle characterisation, are given in [1] and [2] . The data presented here are the percentage recovery rates by mass for each of three serum samples doped with silicon nitride particles, in addition to a control serum sample to which no particles were added, following the isolation process outlined in [1] .…”

Section: Datamentioning

confidence: 99%

Recovery rate data for silicon nitride nanoparticle isolation using sodium polytungstate density gradients

et al. 2018

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The average recovery rate of silicon nitride nanoparticles isolated from serum using the method detailed in previous article “A novel method for isolation and recovery of ceramic nanoparticles and metal wear debris from serum lubricants at ultra-low wear rate” (Lal et al., 2016) [1] was tested gravimetrically by weighing particles doped into serum before and after the isolation process. An average recovery rate of approximately 89.6% (± 7.1 SD) was achieved.

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Quantitative analysis and degradation mechanisms of different protein degradation methods

et al. 2021

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The abrasive debris produced by wear test of artificial joints in vitro is encapsulated by proteins in serum lubricants, which hinder the characterization of debris analysis.One of the key issues of isolating wear debris from serum is degrading the proteins wrapping the wear debris. In this article, the proteins in calf serum were degraded by a strong alkali, a strong acid, and an enzyme. The residual concentration of proteins in calf serum was detected by UV absorption. Quantitative analysis of protein degradation and the protein degradation rate was proposed, following treatment with different degradation reagents and different incubation times. The results showed that when 10 mL of 25% volume calf serum was added with 40 mL of NaOH and incubated at 65 C for 24 h, the protein degradation rate reached a maximum of 95.52%.The protein degradation rate in the solution ranged from 31.86% to 71.64% when a different volume of 37% HCl was added and incubated at 60 C. The highest protein degradation rate was 94.98% in the protease degradation solution. When the protein degradation rate is less than 70%, the particles were coated by protein. When the protein degradation rate was more than 95%, there was no protein coating around the particles. The three protein degradation methods have different processes and protein degradation rates. A suitable method for protein degradation can be selected according to these practical applications.artificial joints, degradation rate, protein degradation, recovery of debris | INTRODUCTIONAfter an artificial joint is implanted into the human body, it is not only soaked by body fluids but is also impacted by and subjected to normal and shear forces. The abrasive particles generated by the wear of joints induce osteoclasts to produce osteolytic factors and induce bone resorption. Aseptic loosening of the prosthesis due to bone dissolution is the most common cause of the failure of the prosthesis. [1][2][3] The different sizes and morphologies of debris reflect the different wear behavior and mechanism of the artificial joint in addition to the different degrees of biological reaction. [4][5][6][7][8] Therefore, a reliable method of isolating debris from serum during artificial joint wear tests in vitro is important to the analysis of debris characteristics.Calf serum has been widely used as the lubricating fluid in vitro for wear tests of artificial joints. [9][10][11] Artificial joint debris binds to proteins in serum by ionization, hydrophobicity, and hydrophilicity. The joint debris is wrapped by multiple layers of proteins called the protein halo. 12-14 Therefore, the degree of protein degradation affects the separation and analysis of the amount and morphology of the debris.A suitable protein degradation method is an important prerequisite for the isolation and analysis of wear debris.Many scholars have researched different protein degradation methods. Different protein degradation methods have different requirements for the amount of strong acid, strong alkali and protease, temperat...

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Recovery of low volumes of wear debris from rat stifle joint tissues using a novel particle isolation method

Cited by 8 publications

References 50 publications

Validation of a novel particle isolation procedure using particle doped tissue samples

Validation of a novel particle isolation procedure using particle doped tissue samples

Recovery rate data for silicon nitride nanoparticle isolation using sodium polytungstate density gradients

Quantitative analysis and degradation mechanisms of different protein degradation methods

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