Recovery of long-term denervated human muscles induced by electrical stimulation

Kern, Helmut; Salmons, Stanley; Mayr, Winfried; Rossini, Katia; Carraro, Ugo

doi:10.1002/mus.20149

Cited by 148 publications

(117 citation statements)

References 12 publications

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“…Although this technique did not distinguish between satellite cells and myonuclei, the finding opens up the possibility that there is no loss of myonuclei even after very long disuse periods. If nuclei and fiber integrity persist even in the extremely atrophic fibers induced by long-term disuse, this might explain the remarkable recovery of muscle strength when nerve activity is restored or substituted by electrodes even after prolonged inactivity both in rodents (46) and in humans (47).…”

Section: Figurementioning

confidence: 99%

In vivo time-lapse microscopy reveals no loss of murine myonuclei during weeks of muscle atrophy

Bruusgaard¹,

Gundersen²

2008

J. Clin. Invest.

151

177

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Numerous studies have suggested that muscle atrophy is accompanied by apoptotic loss of myonuclei and therefore recovery would require replenishment by muscle stem cells. We used in vivo time-lapse microscopy to observe the loss and replenishment of myonuclei in murine muscle fibers following induced muscle atrophy. To our surprise, imaging of single fibers for up to 28 days did not support the concept of nuclear loss during atrophy. Muscles were inactivated by denervation, nerve impulse block, or mechanical unloading. Nuclei were stained in vivo either acutely by intracellular injection of fluorescent oligonucleotides or in time-lapse studies after transfection with a plasmid encoding GFP with a nuclear localization signal. We observed no loss of myonuclei in fast-or slow-twitch muscle fibers despite a greater than 50% reduction in fiber cross-sectional area. TUNEL labeling of fragmented DNA on histological sections revealed high levels of apoptotic nuclei in inactive muscles. However, when costained for laminin and dystrophin, virtually none of the TUNEL-positive nuclei could be classified as myonuclei; apoptosis was confined to stromal and satellite cells. We conclude that disuse atrophy is not a degenerative process, but is rather a change in the balance between protein synthesis and proteolysis in a permanent cell syncytium.

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Section: Figurementioning

confidence: 99%

In vivo time-lapse microscopy reveals no loss of murine myonuclei during weeks of muscle atrophy

Bruusgaard¹,

Gundersen²

2008

J. Clin. Invest.

151

177

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“…3 Intensive regime of electrical stimulation can also result in considerable restitution of mass, excitability and force in denervated muscles more than 20 years after SCI. 4,5 Efficacy of delivered electrical current is dependent on stimulus frequency, amplitude and pulse width. The minimum stimulus frequency that generates a fused muscle response is B12.5 Hz.…”

Section: Introductionmentioning

confidence: 99%

Neuromuscular electrical stimulation of completely paralyzed abdominal muscles in spinal cord-injured patients: a pilot study

et al. 2008

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Study Design: Prospective placebo-controlled. Objective: The effect of abdominal neuromuscular electrical stimulation (NMES) in patients with spinal cord injury. The principal parameters observed in this study are lung capacity, colonic transit, patient satisfaction of used method and of aesthetics effect on abdominal wall. Settings: Centre de Traumatologie et de Réadaptation, Brussels, Belgium. Methods: A total of 10 volunteers participated in this study and were assigned to two groupsFthe effective electrical stimulation group (ESG) and the placebo-controlled group (PG). NMES of abdominal muscles was performed 25 min per day for 8 weeks.Results: NMES significantly decreased forced vital capacity (FVC) in ESG but not in PG. In ESG, colonic transit was accelerated in ascending, transverse and descending colon but transit in rectosigmoideum was not affected. In PG, no variations in colonic transit were observed. Satisfaction scale shows a better influence on aesthetics effect in ESG than in PG.Conclusion: This pilot study shows that NMES of paralyzed abdominal muscles positively affects colonic transit except in rectosigmoideum segment and negatively affects FVC. It could be a simple self-used method to regulate colonic transfer with considerably good cosmetic effect on abdominal wall. However, regular verification of FVC will probably be necessary.

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“…7 Muscle biopsies from patients with spinal cord injury lasting from 9 months to 19 years also contained immature fibers, which were interpreted as regenerative in nature. 8,11,14,15 These fibers had a central nucleus and a thin cytoplasmic rim containing a few myofilaments, and expressed the embryonic isoform of the myosin heavy chain molecule (MHCemb). They represented about 1% of the total myofiber population in these muscle biopsies.…”

mentioning

confidence: 99%

Myogenesis in human denervated muscle biopsies

2007

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Electron microscopic studies of long-term denervated rat muscles have identified very small, immature myofibers that are believed to arise from detached satellite cells that have fused to form new fibers within the interstitial space. At present, it is unknown whether and to what extent equivalent fibers exist in denervated human muscle. Serial sections of muscle biopsies from 66 patients diagnosed with polyneuropathy or amyotrophic lateral sclerosis were immunolabeled with anti-NCAM and antineonatal myosin heavy chain monoclonal antibodies that are both neurally and developmentally regulated. We evaluated 200 myofibers in each section. Of the biopsy specimens, 75% contained small myofibers that showed a thin perinuclear cytoplasmic rim. Small fibers expressing neonatal myosin heavy chain (MHCnϩ) were found in all of these biopsies (100%) and NCAMϩ fibers in 98%. The percentage of MHCnϩ small fibers averaged 82% and NCAMϩ small myofibers averaged 40%. The percentage of NCAMϩ small fibers was significantly lower than that of MHCnϩ fibers. In contrast, the percentage of MHCnϩ vs. NCAMϩ angular atrophic fibers did not show a significant difference. A substantial subset of neurogenic biopsies showed small fibers that differ from angular atrophic fibers both in size and expression pattern of MHCn and NCAM. Myogenesis appears to be a frequent finding in neurogenic atrophy.

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Recovery of long-term denervated human muscles induced by electrical stimulation

Cited by 148 publications

References 12 publications

In vivo time-lapse microscopy reveals no loss of murine myonuclei during weeks of muscle atrophy

In vivo time-lapse microscopy reveals no loss of murine myonuclei during weeks of muscle atrophy

Neuromuscular electrical stimulation of completely paralyzed abdominal muscles in spinal cord-injured patients: a pilot study

Myogenesis in human denervated muscle biopsies

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