Recovery of Lactic Acid from Dilute Aqueous Solutions Using a Binary Mixture of Tri-<i>n</i>-octylphosphine Oxide (TOPO) or Dioctylamine (DOA) with Different Solvents

Uslu, Hasan; Hasret, Erdem; Kırbaşlar, Ş. İsmail

doi:10.1021/acs.jced.8b00857

Cited by 3 publications

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References 13 publications

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“…However, they are hampered with product inhibition during biological production because of changes in broth pH, or system toxicity, or through absorptive removal of a liquid phase which is challenging (Djas & Henczka, 2018; Janssen et al, 2007). Nevertheless, to circumvent such challenges different liquid‐liquid systems, physical configurations, nutrient sources, and microorganisms have been employed to help increase system productivity and yield, eyeing future scalability of this extractive fermentation process (Abdel‐Rahman et al, 2019; Othman, Ariff, Wasoh, Kapri, & Halim, 2017; Peterson & Daugulis, 2013, 2014; Uslu, Hasret, & Klrbaşlar, 2018). Hence, novel physical configurations, modes of operation, different liquid‐liquid mixtures and nutrient sources should be investigated to increase the competitive nature toward system sustainability and scalability.…”

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confidence: 99%

Design and evaluation of a continuous semipartition bioreactor for in situ liquid‐liquid extractive fermentation

Teke

Pott

2020

Biotech & Bioengineering

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Extractive fermentation (or in situ product removal (ISPR)) is an operational method used to combat product inhibition in fermentations. To achieve ISPR, different separation techniques, modes of operation and physical reactor configurations have been proposed. However, the relative paucity of industrial application necessitates continued investigation into reactor systems. This article outlines a bioreactor designed to facilitate in situ product extraction and recovery, through adapting the reaction volume to include a settler and solvent extraction and recycle section. This semipartition bioreactor is proposed as a new mode of operation for continuous liquid-liquid extractive fermentation. The design is demonstrated as a modified bench-top fermentation vessel, initially analysed in terms of fluid dynamic studies, in a model two-liquid phase system. A continuous abiotic simulation of lactic acid (LA) fermentation is then demonstrated. The results show that mixing in the main reaction vessel is unaffected by the inserted settling zone, and that the size of the settling tube effects the maximum volumetric removal rate. In these tests the largest settling tube gave a potential continuous volumetric removal rate of 7.63 ml/min; sufficiently large to allow for continuous product extraction even in a highly productive fermentation. To demonstrate the applicability of the developed reactor, an abiotic simulation of a LA fermentation was performed. LA was added to reactor continuously at a rate of 33ml/h, while continuous in situ extraction removed the LA using 15% trioctylamine in oleyl alcohol. The reactor showed stable LA concentration of 1 g/L, with the balance of the LA successfully extracted and recovered using back extraction. This study demonstrates a potentially useful physical configuration for continuous in situ extraction.

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