Recovery of Known-Age Hyalella Azteca (Amphipoda) From Sediment Toxicity Tests

Tomasovic, Michael J.; Dwyer, F. James; Greer, I. Eugene; Ingersoll, Christopher G.

doi:10.1897/1552-8618(1995)14[1177:rokhaa]2.0.co;2

Cited by 15 publications

(24 citation statements)

References 2 publications

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“…Cultures were maintained at 23ЊC (Ϯ1ЊC) at light intensity of about 500 lux and were fed Tetramin fish food (Tetra-Werke, Melle, Germany) and presoaked maple leaves ad libitum. Each aquarium contained six nylon substrates (20-cm-diameter sections of nylon ''Coiled-web material''; 3-M, St. Paul, MN, USA) [27]. Known-age amphipods were obtained by sieving organisms from the mixed-aged culture through a no.…”

Section: Culture Of Amphipodsmentioning

confidence: 99%

“…Mature amphipods retained on the sieve were pipeted into a no. 40 (425-m mesh) sieve, placed in a shallow glass pan containing water, and left overnight to release newborn amphipods [27]. After 24 h, the Ͻ24-h-old amphipods (neonates) were rinsed through the no.…”

Section: Culture Of Amphipodsmentioning

confidence: 99%

“…The first design considered was a continuation of the 28-d sediment exposures described in Ingersoll et al [20] for an additional 2 weeks to determine the number of young produced in the first brood. The limitation of this design is the difficulty in quantitatively isolating young amphipods from sediment [27]. A second design considered was extension of the 28-d sediment exposure for an additional month or longer until several broods are released.…”

Section: Introductionmentioning

confidence: 99%

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Use of Sublethal Endpoints in Sediment Toxicity Tests With the Amphipod Hyalella Azteca

Ingersoll¹,

Brunson²,

Dwyer³

et al. 1998

Environ Toxicol Chem

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101

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Abstract-Short-term sediment toxicity tests that only measure effects on survival can be used to identify high levels of contamination but may not be able to identify marginally contaminated sediments. The objective of the present study was to develop a method for determining the potential sublethal effects of contaminants associated with sediment on the amphipod Hyalella azteca (e.g., reproduction). Exposures to sediment were started with 7-to 8-d-old amphipods. On day 28, amphipods were isolated from the sediment and placed in water-only chambers where reproduction was measured on day 35 and 42. Typically, amphipods were first in amplexus at about day 21 to 28 with release of the first brood between day 28 to 42. Endpoints measured included survival (day 28, 35, and 42), growth (as length and weight on day 28 and 42), and reproduction (number of young/female produced from day 28 to 42). This method was used to evaluate a formulated sediment and field-collected sediments with low to moderate concentrations of contaminants. Survival of amphipods in these sediments was typically Ͼ85% after the 28-d sediment exposures and the 14-d holding period in water to measure reproduction. Reproduction was more variable than growth; hence, more replicates might be needed to establish statistical differences among treatments. Previous studies have demonstrated that growth of H. azteca in sediment tests often provides unique information that can be used to discriminate toxic effects of exposure to contaminants. Either length or weight can be measured in sediment tests with H. azteca. However, additional statistical options are available if length is measured on individual amphipods, such as nested analysis of variance that can account for variance in length within replicates. Ongoing water-only studies testing select contaminants will provide additional data on the relative sensitivity and variability of sublethal endpoints in toxicity tests with H. azteca.

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Section: Culture Of Amphipodsmentioning

confidence: 99%

Section: Culture Of Amphipodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Use of Sublethal Endpoints in Sediment Toxicity Tests With the Amphipod Hyalella Azteca

Ingersoll¹,

Brunson²,

Dwyer³

et al. 1998

Environ Toxicol Chem

Self Cite

101

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show abstract

“…Amphipods were mass cultured at 23°C with a luminance of about 800 lux in 80-L glass aquariums containing 50 L of ECRC well water (hardness 283 mg/L as CaCOs, alkalinity 255 as CaC03, pH 7.8) with a nylon, coiled-web material (3M Corp., Saint Paul, MN) as substrates (Tomasovic et al 1995). Known-age amphipods were isolated by placing mixed-aged adults in a #35 US Standard size sieve (5-mm mesh) inside a pan containing about 2 cm of well water.…”

Section: Culturing Of Test Organismsmentioning

confidence: 99%

Assessing Sediment Toxicity from Navigational Pools of the Upper Mississippi River Using a 28-Day Hyalella azteca Test

Kemble

Brunson

Canfield

et al. 1998

Archives of Environmental Contamination and Toxicology

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Abstract.To assess the extent of sediment contamination in the Upper Mississippi River (UMR) system after the flood of 1993, sediment samples were collected from 24 of the 26 navigational pools in the river and from one site in the Saint Croix River in the summer of 1994. Whole-sediment tests were conducted with the amphipod Hyalella azteca for 28 days measuring the effects on survival, growth, and sexual maturation. Amphipod survival was significantly reduced in only one sediment (13B) relative to the control and reference sediments. Body length of amphipods was significantly reduced relative to the control and reference sediments in only one sample (26C). Sexual matura tion was not significantly reduced in any treatment when compared to the control and reference sediments. No significant correlations were observed between survival, growth, and maturation to either the physical or chemical characteristics of the sediment samples from the river. When highly reliable effect range medians (ERMs) were used to evaluate sediment chemis try, 47 of 49 (96%) of the samples were correctly classified as nontoxic. These results indicate that sediment samples from the Upper Mississippi River are relatively uncontaminated com pared to other areas of known contamination in the United States.

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“…Documentation exists (Society of Environmental Toxicology and Chemistry, 1994;Tomasovic, et. al., 1995) which suggests that amphipods decompose beyond recognition in less than 24 hours post-mortem.…”

Section: Data Analysis Uncertaintiesmentioning

confidence: 99%

Report on the remedial investigation of Bear Creek Valley at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee. Volume 6: Appendix G -- Baseline ecological risk assessment report

1996

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Recovery of Known-Age Hyalella Azteca (Amphipoda) From Sediment Toxicity Tests

Cited by 15 publications

References 2 publications

Use of Sublethal Endpoints in Sediment Toxicity Tests With the Amphipod Hyalella Azteca

Use of Sublethal Endpoints in Sediment Toxicity Tests With the Amphipod Hyalella Azteca

Assessing Sediment Toxicity from Navigational Pools of the Upper Mississippi River Using a 28-Day Hyalella azteca Test

Report on the remedial investigation of Bear Creek Valley at the Oak Ridge Y-12 Plant, Oak Ridge, Tennessee. Volume 6: Appendix G -- Baseline ecological risk assessment report

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