Recovery of increased weights of the liver and kidneys by cessation of D-allulose feeding in Wistar rats

Abstract

Rare sugar D-allulose prevents obesity; however, an excessive and continuous intake of D-allulose may induce weight increases in the liver and kidney without apparent pathological and functional abnormalities. Conversely, there has not been reported about how these parameters will change after cessation of D-allulose intake. In this study, effects of a 10-week D-allulose cessation on liver and kidneys weights and biomarkers were investigated in rats previously fed a D-allulose containing diet for 4 weeks. Wistar rats were fed a control diet (C, n=16) or a 3% D-allulose diet (DA, n=16) for 4 weeks, and then the half of rats in the C and DA subgroups were dissected, while the other half of rats were fed the control diet for 10 weeks (C-C and DA-C, n=8, respectively). At the end of the first 4 weeks period, halves of rats in each diet group were euthanized, and the serum, urine, liver, and kidneys were used for pathological and biochemical analyses. The remaining rats were also similarly treated at the end of latter 10 weeks treatment. At week 4, the relative weights of the liver and kidneys were higher in the DA group than in the C group, but these differences were disappeared by cessation of D-allulose. No abnormal parameters related to liver and kidneys functions were observed in the serum and urine. These findings suggested that D-allulose-induced increases in the liver and kidneys weights could be recovered to the normal levels by D-allulose cessation without accompanying functional and pathological abnormalities.

Safety evaluation of 12-week continuous ingestion of D-allulose in borderline diabetes and type 2 diabetes

“…In addition, D-allulose has been reported to suppress elevation of postprandial blood glucose (Hayashi et al, lulose acts by inhibiting α-glucosidase activity (Matsuo and Izumori, 2006) and increasing glucose uptake to the liver by facilitating glucokinase translocation from the nucleus to the cytoplasm in the liver (Hossain et al, 2011). D-allulose has already been clarified not to cause mutagenesis nor toxicity (Matsuo et al, 2002;Ochiai et al, 2019). The maximum non-effective level of D-allulose in causing diarrhea in human subjects was approximately 0.50-0.60 g per kg body weight (Iida et al, 2007).…”

Effect of simultaneous intake of rare sugars allitol and D-allulose on intra-abdominal fat accumulation in rats

“…In our previous study of chronic toxicity using rats, the administration of 3% D-allulose resulted in kidney hypertrophy without any adverse health effects (Yagi & Matsuo, 2009). In addition, we suggested that feeding rats a diet containing 3% D-allulose for 4 weeks could increase the weights of the liver and kidney without any pathological damage, while 10 weeks of D-allulose interruption reversed these effects (Ochiai et al, 2019). In a clinical study evaluating 12-week ingesting of an isomerized carbohydrate containing 6% D-allulose, Hayashi et al (2014) reported the absence of adverse effects on studies of lipid and carbohydrate metabolism or abnormal blood parameters, and renal and hepatic functions.…”