Recovery of gamma irradiated <i>Rhizopus stolonifer</i> sporangiospores during autoinhibition of germination

Sommer, N. F.; Creasy, Min T.; Romani, Roger J.; Maxie, E.C.

doi:10.1002/jcp.1030610110

Cited by 14 publications

(6 citation statements)

References 12 publications

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“…This is supported by our earlier results (Thanh and Nout 2002, 2004) indicating that not all fluorescent spores could form colonies. Furthermore, there is no correlation between germ tube formation and colony‐forming ability, because many spores retained the ability to form short germ tubes, but they grew no further; similar events had been reported for sporangiospores of R. stolonifer (Sommer et al. 1963) and conidia of N. crassa (Schmit and Brody 1976).…”

Section: Discussionmentioning

confidence: 56%

“…We observed that even if a spore is able to form a germ tube this does not always mean that it can complete the whole process and can give rise to a colony. In practical studies on tempe starter, our results based on many experiments showed that there is no correlation between the ability to form germ tubes and the formation of colonies; many spores are able to form germ tubes, but they grow no further, as was also observed in sporangiospores of Rhizopus stolonifer (Sommer et al. 1963) and conidia of Neurospora crassa (Schmit and Brody 1976).…”

Section: Introductionmentioning

confidence: 81%

“…2 Proline uptake rates nmol min )1 of dormant spores in proline (pure) and in proline + alanine (mixture) solution for 120 min at 37°C (in the mixture of proline + alanine solution, the concentration of proline was equal to alanine). , In Pro (pure) solution and j, in Pro + Ala solution sporangiospores of R. stolonifer (Sommer et al 1963) and conidia of N. crassa (Schmit and Brody 1976).…”

Section: Discussionmentioning

confidence: 99%

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Effect of individual amino acids and glucose on activation and germination of Rhizopus oligosporus sporangiospores in tempe starter

2005

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Aim: To understand the conditions promoting activation and germination of spores, and to contribute to the control of tempe starters. Methods and Results: Using microscopic counts of fluorescent labelled spores, the following results were obtained: (1) L-alanine plays an important role (of the same order as that of peptone) in stimulation of germination of dormant spores. Alanine can satisfy the requirements of carbon as well as nitrogen for spore germination; (2) L-proline, on the other hand, inhibits alanine uptake presumably by blocking/congesting transporters of spore cells, resulting in apparent low viability on agar media; (3) L-leucine and L-isoleucine slightly favour spore germination while L-arginine and L-lysine do not have any stimulating effect; (4) The stimulatory role of glucose was only evident in the presence of phosphate (in minimal medium); when glucose is used in the absence of phosphate, either alone or in combination with single amino acids its role is hardly distinguishable; (5) Phosphate plays a facilitating role in spore germination. Conclusions: Glucose and amino acids play important roles in activation and germination of sporangiospores of Rhizopus oligosporus in tempe starter (stored for 12 months). The ability and rate of germination of dormant/old sporangiospores of R. oligosporus, depend on their ability for uptake of individual amino acids and/or glucose. Significance and Impact of Study: New light was shed on the counteractive role of proline and the stimulating effect of phosphate. Soybeans subjected to traditional preparation for tempe making are heavily leached; germination of starter spores on such beans is sub-optimal, and bean processing could be optimized.

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Section: Discussionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Effect of individual amino acids and glucose on activation and germination of Rhizopus oligosporus sporangiospores in tempe starter

2005

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“…It is clear that sporangiospores of R. stolonifer can synthesize abundant enzymes capable of macerating plant tissue, even after the loss through irradiation of their ability to form colonies. A striking "rescue" of the colonyforming potential of sporangiospores occurs if germination is inhibited after irradiation (Sommer et al, 1963). Nevertheless, pectolytic enzyme production by the spore continues unabated after the potential for unlimited growth has been irreversibly lost.…”

Section: Discussionmentioning

confidence: 99%

“…R. stolonifer cultures were grown m dose exceeded about 1 megarad. The ability to produce 300-ml Erlenmeyer flasks on 100 -ml of cherry or V-8 juice pectolytic enzymes was found to be more radiationagar medium, and sporangiospores were harvested as resistant than the potential for colony formation or the previously described (Sommer et al, 1963). The freshly ability to germinate.…”

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confidence: 94%