Recovery of Edwardsiella ictaluri from danio (Danio devario)

Waltman, W. D.; Shotts, Emmett B.; Blazer, Vicki S.

doi:10.1016/0044-8486(85)90176-0

Cited by 51 publications

(31 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could be due to the small size of the zebrafish or because the mortalities in this trial were acute; cutaneous lesions are associated with chronic infections in channel catfish (Newton et al 1989). The lesions described in the first report of Edwardsiella ictaluri in the Bengal danio (Blazer et al 1985) were comparable to those observed in the chronic form of the disease in channel catfish ). Nasal pit lesions and the ensuing encephalopathies are usually associated with immersion (Newton et al 1989) and natural (waterborne) infections Noga 1996;Plumb 1999).…”

Section: Discussionsupporting

confidence: 69%

Evaluation of Zebrafish Danio rerio as a Model for Enteric Septicemia of Catfish (ESC)

et al. 2007

View full text Add to dashboard Cite

Zebrafish (also known as zebra danio) Danio rerio were injected intramuscularly with Edwardsiella ictaluri at doses of 6 x 10(3), 6 x 10(4), or 6 x 10(5) colony-forming units per gram (CFU/g) or sterile phosphate-buffered saline (sham) or were not injected. Mortality occurred from 2 to 5 d postinjection (dpi) at rates of 0, 76.6, and 81.3% for the low, medium, and high doses, respectively, and E. ictaluri was isolated from dead fish. Survivors were sampled at 10 dpi and E. ictaluri was not isolated. Sham-injected and noninjected controls did not suffer mortality. Histopathology trials were performed in which zebrafish were injected with 1 x 10(4) CFU/g or sham-injected and sampled at 12, 24, 48, 72, and 96 h postinjection for histological interpretation. Collectively, these zebrafish demonstrated increasing severity of splenic, hepatic, cardiac, and renal interstitial necrosis over time. To evaluate the progression of chronic infection, zebrafish were injected with 1 x 10(2) CFU/g and held for 1 month postinjection. Beginning at 12 dpi and continuing for an additional 2 weeks, zebrafish demonstrated abnormal spiraling and circling swimming behaviors. Histopathology demonstrated necrotizing encephalitis. In immersion trials, zebrafish were exposed to low, medium, and high doses (averaging 1.16 x 10(5), 1.16 x 10(6), and 1.16 x 10(7) CFU/mL of tank water) of E. ictaluri for 2 h. Mortality occurred from 5 to 9 d postexposure at rates of 0, 3.3, and 13.3% for the low, medium, and high doses, respectively; E. ictaluri was isolated from dead fish. Channel catfish Ictalurus punctatus exposed to the medium doses suffered 100% mortality, and E. ictaluri was isolated from these fish. This study demonstrates the potential use of zebrafish as a model for E. ictaluri pathogenesis.

show abstract

Section: Discussionsupporting

confidence: 69%

Evaluation of Zebrafish Danio rerio as a Model for Enteric Septicemia of Catfish (ESC)

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Although naturally occurring disease has also been reported in some freshwater ornamental species, i.e. rosy barbs Puntius conchonius (Humphrey et al, 1986), danio Danio devario (Waltman et al, 1985), and green knife fish Eigemannia virescens (Plumb, 1999), or farmed rainbow trout Oncorhynchus mykiss (Keskın et al, 2004), catfish is specifically susceptible to E. ictaluri (Plumb, 1999). Stanley et al (1994) reported that the LD 50 values of the virulent strains were 10 3 -10 5 CFU/fish in ip-injected channel catfish.…”

Section: Discussionmentioning

confidence: 99%

Outbreaks of Edwardsiella ictaluri Infection in Ayu Plecoglossus altivelis in Japanese Rivers

et al. 2008

View full text Add to dashboard Cite

ABSTRACT-From middle August to early October in 2007, mortalities in wild ayuPlecoglossus altivelis occurred in the rivers of Tokyo Metropolis, Yamaguchi Prefecture and Hiroshima Prefecture, Japan. Hemorrhagic ascites was observed in almost all the examined fish. Some fish showed reddening of body surface, anus or bases of fins and exophthalmos. A single species of bacteria was isolated from the kidney, spleen and ascites of all the examined fish and identified as Edwardsiella ictaluri. Pathogenicity against ayu of the bacterial isolates was demonstrated by experimental infections, with the LD 50 being approximately 10 4 CFU/fish. The dead fish exhibited similar disease signs to those of naturally affected fish. From these results, it was concluded that the present mortality of wild ayu was caused by E. ictaluri infection. This is the first report on E. ictaluri infection of fish in Japan.

show abstract

“…Danio devario, a common aquarium fish (Waltman et al, 1985). The isolate is biochemically identical to strains isolated from catfish.…”

Section: Introductionmentioning

confidence: 94%

Pathology associated with Edwardsiella ictaluri in catfish, Ictalurus punctatus Rafinesque, and Danio devario (Hamilton‐Buchanan, 1822)

Blazer

Shotts

Waltman

1985

Journal of Fish Biology

View full text Add to dashboard Cite

The pathology associated with infections of Edwardsiellu ictaluri in a new host, the danio, is described and compared to that observed in the channel catfish. In catfish the acute stage was characterized by petechial haemorrhage of the jaw, ventral body surface, and at the base of the fins. In chronic cases a characteristic finding was an erosion of skin and muscle overlying the skull, exposing bone and occasionally the brain. Histologically there was a diffuse granulomatous inflammation of the olfactory bulb and the telencephalon. In the catfish the infection was often systemic, involving intestine, liver, spleen, and occasionally kidney. In the danio no gross lesions were observed. Infected fish exhibited an erratic, spinning-type swimming behaviour. Histologically, lesions usually were confined to the brain. They consisted of an acute, primarily granulocytic inflammation of the medulla oblongata (rhomboencephalon). Only one fish showed evidence of a systemic infection.

show abstract

Recovery of Edwardsiella ictaluri from danio (Danio devario)

Cited by 51 publications

References 4 publications

Evaluation of Zebrafish Danio rerio as a Model for Enteric Septicemia of Catfish (ESC)

Evaluation of Zebrafish Danio rerio as a Model for Enteric Septicemia of Catfish (ESC)

Outbreaks of Edwardsiella ictaluri Infection in Ayu Plecoglossus altivelis in Japanese Rivers

Pathology associated with Edwardsiella ictaluri in catfish, Ictalurus punctatus Rafinesque, and Danio devario (Hamilton‐Buchanan, 1822)

Contact Info

Product

Resources

About