Recovery of cell cultures and their biosynthetic capacity after storage of Dioscorea deltoidea and Panax ginseng cells in liquid nitrogen

Butenko, R. G.; Popov, A. S.; Волкова, Л. И.; Chernyak, N. D.; Nosov, A. M.

doi:10.1016/0304-4211(84)90019-1

Cited by 45 publications

(18 citation statements)

References 7 publications

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“…In contrast, lower accumulation of dry cell weight was recorded for cryopreserved Taxus chinensis cell culture when compared with the untreated control in the course of 40-day cultivation, however, paclitaxel production was retained at the same level [ 246 ]. Diosgenine, sitosterol and stigmasterols content remained unchanged after cryopreservation of Dioscorea deltoidea cell cultures [ 131 ]. The same profi les of the relative DNA content have been recorded for cell cultures of Ginkgo biloba in the course of 24-month cultivation followed by cryopreservation [ 237 ].…”

Section: Cryopreservation Of Plant Cell Cultures With Enhanced Isoprementioning

confidence: 97%

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Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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Plant cell culture is traditionally viewed as a unique artifi cially created biological system representing a heterogenous population of dedifferentiated cells. This system undergoes a continuous process of autoselection based on the intensity and stability of cell proliferation. We discuss here the details of formation and regulation of isoprenoid biosynthesis in plant cell in vitro based on literature survey and our research. Obviously, secondary metabolism differs in cell culture compared to the plant per se , because in cell culture metabolites are synthesized and compartmentalized within a single heterotrophic cell with sparse or underdeveloped vacuoles and plastids. For example, in plant cell cultures isoprenoid biosynthesis via MVA pathway was found to be more active than via plastid-localized MEP pathway. Also, it was hypothesized that cell cultures preferably produce metabolites, which promote cell proliferation and growth. Indeed, cell cultures of Dioscorea deltoidea produced mainly furostanol glycosides, which promoted cell division. Triterpene glycosides (ginsenosides) in the cell cultures of various Panax species are represented mainly by Rg-and Rb-groups. Rb ginsenosides are predominantly found as malonyl-esters that may infl uence their intracellular localization.Despite the difference in the isoprenoid composition in plant and cell culture the latter became an attractive source of phytochemicals as an alternative to plant harvesting. We provide in this chapter the guidelines to biotechnological production of plant isoprenoids using plant cell cultures and discuss the optimal methods of bioreactor-based cultivation and cryopreservation of plant cell collections.

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Section: Cryopreservation Of Plant Cell Cultures With Enhanced Isoprementioning

confidence: 97%

“…The fi rst suspension cell culture from P. ginseng was derived from the callus of a cambial origin in 1970 [ 131 ].…”

Section: Physiological Characteristics Of Cell Cultures Of Different mentioning

confidence: 99%

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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“…Most of the results about cryopreservation have been obtained from conventional techniques on suspension cells of medicinal yam, D. deltoidea being the most used ( Butenko et al, 1984;Popov and Fedorovskii, 1992;Popov and Volkova, 1994). More recent works have been done on rapid cryopreservation of callus (Chulafich et al, 1994) On the other hand, ORSTOM ability in different aspects of the long term conservation on tropical plants (Engelmann, 1991), and on encapsulation/dehydration technique applied on coffee, cassava, oil palm...etc, permitted to apply the process on apical shoot-tips of in vitro plantlets of yam (Malaurie and Trouslot 1996).…”

Section: Long-term Conservationmentioning

confidence: 99%

Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm

Malaurie¹,

Trouslot²,

Berthaud³

et al. 1998

Electron. J. Biotechnol.

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Yam edible tubers feed million of peoples in the intertropical area, where they represent 12% of human feeding. However, as a vegetatively propagated crop, yam is seriously affected by an accumulation of pathogens. Establishing in vitro germplasm collection is a process that cleans the plants from all diseases but viruses. It gives a good control on the preservation of the yam genetic resources and facilitates international exchanges of healthy plant material.Two kinds of in vitro germplasm preservation were considered : slow growth condition culture for midterm preservation, and cryopreservation using the encapsulation/dehydration technique for long-term preservation. Virus eradication was approached by meristem culture and chemo and thermotherapy. Production of virus-free plants was controlled by ELISA. Mid-term conservation of yam germplasm is used routinely, and from these conditions a direct acclimatization is possible. On the cryopreservation aspect, experiments are under way to apply the optimized protocol to genotypes which are more representative of the diversity, to insure a routinely use. More work can be conducted now on virus eradication, We succeeded in the introduction

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“…For cryoprotection, various substances are employed, individually or in binary or ternary mixtures. Sucrose (7 to 25%) is used with Panax ginseng cell suspensions (Butenko et al, 1984), DMSO (5%) with rice cells (Celia et al, 1982). A mixture of cryoprotectants is oftenly more efficient than only one component at the same total osmolarity.…”

Section: Cell Suspensionsmentioning

confidence: 99%

“…As regards post-thaw treatments, cells can eventually be washed in order to remove the cryoprotectants (Butenko et al, 1984). However, this treatment is in the majority of the cases deleterious to the survival of the cells due to the osmotic shock created.…”

Section: Cell Suspensionsmentioning

confidence: 99%

In vitro conservation of tropical plant germplasm — a review

Engelmann

1991

Euphytica

175

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In vitro medium term conservation of tropical plant germplasm is used routinely in many laboratories. Growth reduction is achieved by modifying various parameters, such as temperature, culture medium, gaseous environment. For long term conservation, cryopreservation (i.e. storage in liquid nitrogen, -196 ° C) is the only current method available. Each successive step of the process requires precise conditions which have to be defined for each material. Cryopreservation protocols have been set up for more than 40 tropical species. Results obtained with various culture systems such as cell suspensions, protoplasts, calluses, meristems and embryos are discussed. The first example of the large scale application of cryopreservation (oil palm somatic embryos) is presented.

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Recovery of cell cultures and their biosynthetic capacity after storage of Dioscorea deltoidea and Panax ginseng cells in liquid nitrogen

Cited by 45 publications

References 7 publications

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm

In vitro conservation of tropical plant germplasm — a review

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