Recovery of Acetylcholinesterase Activity in the Common Carp ( Cyprinus carpio L.) After Inhibition by Organophosphate and Carbamate Compounds

Dembele, Korami; Haubruge, Éric; Gaspar, Charles

doi:10.1007/s001289900934

Cited by 27 publications

(10 citation statements)

References 21 publications

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“…4a, b). This is in good agreement with the fact that several classes of pesticides (Davies and Cook 1993;Dembele et al 1999;Reinecke and Reinecke 2007;Schreck et al 2008) and heavy metals (e.g. Guilhermino et al 1998;Frasco et al 2005;Elumalai et al 2007;Cunha et al 2007) are anti-ChE substances in several vertebrate and invertebrate species.…”

Section: Che Characterizationsupporting

confidence: 79%

Biochemical characterization of cholinesterases in Enchytraeus albidus and assessment of in vivo and in vitro effects of different soil properties, copper and phenmedipham

et al. 2010

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Enchytraeus albidus are important organisms of the soil biocenosis, used as standard test species in environmental risk assessment. The inhibition of cholinesterases (ChE) activity of several species has been widely used to assess the exposure and effects of anti-cholinesterase environmental contaminants. Several studies have shown the association between ChE activity inhibition and adverse effects on behaviour and survival. Extensive studies addressing survival and behavioural endpoints, as well as other biomarkers, have been done in E. albidus with different types of soil contaminants. The main objectives of this study were: (1) to characterize biochemically the ChE present in the soluble post-mitochondrial fraction of E. albidus whole body homogenates, using different substrates and selective inhibitors; (2) to assess the in vivo effects of copper, phenmedipham and different soil properties (pH, organic matter, clay) on the ChE activity; (3) to assess the in vitro effects of copper and phenmedipham on the ChE activity. The results suggest the presence of one ChE in the soluble post-mitochondrial fraction of E. albidus whole body homogenates, which displays properties of both acetylcholinesterase and pseudocholinesterase considering the typical mammalian enzymes. It is also shown that ChE activity is not inhibited by exposure to different soil properties and that copper and phenmedipham inhibited ChE activity both in in vivo and in in vitro conditions and therefore ChE inhibition seems to be a robust biomarker for this herbicide and this heavy metal. This study showed that ChE activity in E. albidus might be correlated to previously determined higher level effects like survival and reproduction, as well as avoidance behaviour.

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Section: Che Characterizationsupporting

confidence: 79%

Biochemical characterization of cholinesterases in Enchytraeus albidus and assessment of in vivo and in vitro effects of different soil properties, copper and phenmedipham

et al. 2010

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“…In some species, fish survival has been observed even in brain AChE activity inhibition of 90-95% (Sturm et al, 2000;Fulton and Key, 2001;Ferrari et al, 2004). Moreover, several reports have indicated that AChE inhibition by herbicides and insecticides may be reversible (Dembélé et al, 1999;Cattaneo et al, 2011), suggesting de novo protein synthesis or molecular rearrangements in the enzyme structure.…”

Section: Ache Activity In the Anterior Section Of C Decemmaculatusmentioning

confidence: 97%

Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus

Menéndez-Helman

Ferreyroa

Afonso

et al. 2015

Ecotoxicology and Environmental Safety

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“…The toxicity of organophophate and carbamate pesticides toward AChE has been extensively studied both in insects and vertebrates (Haubruge and Toutant, 1997;Bocquene et al, 1995), however, antidote prospect using therapeutic has not been seriously investigated in fishes and other aquatic organisms except in natural condition where the insecticides was discontinuous (Dembele et al, 1999). The mode of action of these insecticides is to phosphorylate or carbamilate AChE (Fleming, 1981).…”

Section: Mortalitymentioning

confidence: 99%

“…The mode of action of these insecticides is to phosphorylate or carbamilate AChE (Fleming, 1981). This results in the increase of acetylcholine levels at the nerve synapse causing continual stimulation of the fibers and eventual failure of nerves to repolarize (Dembele et al, 1999).…”

Section: Mortalitymentioning

confidence: 99%

Therapeutic potential of Garcinia kola with reference to the restoration of inhibited acetylcholinesterase activities in induced Clarias gariepinus

Ikpesu

2014

Beni-Suef University Journal of Basic and Applied Sciences

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a b s t r a c tThis study was conducted to assess the antidotal prospect of Garcinia kola seeds extract in restoring the activity of inhibited Acetylcholinesterase. This was done by inducing Clarias gariepinus with the enzyme inhibitor (glyphosate pesticide formulation). The fish divided into six groups were exposed different treatments; the pesticide alone, the G. kola seed extract alone and different mixture of the pesticide and G. kola seeds extract. AChE activities in the brain, liver and serum of the fish were measured in the experimental and control fish on day À7, 14, 21 and 28th by the colorimetric method. The enzyme was significantly (p < 0.05) inhibited in the glyphosate formulation test alone and in group IV treatment (0.16 mg/L glyphosate formulation with 150 mg/L of extract). The inhibition percentages of AChE ranged for the brain, liver and serum between 40.7e59.4%, 50e57% and 27.5e51.3%, respectively. The AChE activities were however, recovered in G. kola seeds extract treated aquaria, and were dose, time dependent and organ specific. Modifications of this enzyme may leads to increased perspiration, increased salivation, tearing, blurred vision, abdominal cramping, diarrhea, and if severe enough, death from respiratory depression. This investigation had revealed the therapeutic significance of G. kola seeds extract, by stabilizing the enzyme activity in the investigated fish. Further investigation is required to measure the concentrations of acetylcholine at cholinergic synaptic junction in fish and mammals induced with ant-cholinesterase agent and the possibility of its restoration using G. kola seeds.

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Recovery of Acetylcholinesterase Activity in the Common Carp ( Cyprinus carpio L.) After Inhibition by Organophosphate and Carbamate Compounds

Cited by 27 publications

References 21 publications

Biochemical characterization of cholinesterases in Enchytraeus albidus and assessment of in vivo and in vitro effects of different soil properties, copper and phenmedipham

Biochemical characterization of cholinesterases in Enchytraeus albidus and assessment of in vivo and in vitro effects of different soil properties, copper and phenmedipham

Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus

Therapeutic potential of Garcinia kola with reference to the restoration of inhibited acetylcholinesterase activities in induced Clarias gariepinus

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