1998
DOI: 10.1016/s0723-2020(98)80040-4
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Recovery of a Nitrosomonas-like 16S rDNA Sequence Group from Freshwater Habitats

Abstract: In order to study the diversity of ammonia-oxidising bacteria in freshwater habitats, including sediments, a molecular approach focused on the sequencing of 16S rDNA was adopted. 16S rDNA sequences showing affinity with the beta-subgroup of ammonia-oxidising bacteria were recovered by specific PCR of directly isolated DNA from freshwater samples, and samples from brackish water and Glyceria maxima rhizosphere were included in the analysis for comparison. The ammonia oxidiser-like sequences recovered from sever… Show more

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Cited by 78 publications
(97 citation statements)
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“…Our data are in agreement with those of previous studies demonstrating that pure cultures within this group are adapted to low ammonium concentrations (35). Sequencing analyses in other studies also revealed a dominance of N. oligotropha-like AOB in freshwater environments located in cold temperate European regions (16,25,64). In addition to N. oligotropha-like AOB, we found that AOB affiliated with Nitrosospira AmoA cluster 4 (44% of the clones) ( Table 4) coexisted in LS biofilm enrichments incubated at 13°C.…”
Section: Discussionsupporting
confidence: 93%
“…Our data are in agreement with those of previous studies demonstrating that pure cultures within this group are adapted to low ammonium concentrations (35). Sequencing analyses in other studies also revealed a dominance of N. oligotropha-like AOB in freshwater environments located in cold temperate European regions (16,25,64). In addition to N. oligotropha-like AOB, we found that AOB affiliated with Nitrosospira AmoA cluster 4 (44% of the clones) ( Table 4) coexisted in LS biofilm enrichments incubated at 13°C.…”
Section: Discussionsupporting
confidence: 93%
“…While the freshwater/terrestrial strains (e.g. Nitrosomonas europaea) were well separated from our clones in the tree, our clones clustered with other cloned sequences obtained by others from freshwater-lake and sediment environments (Speksnijder et al 1998). Some of the Elkhorn Slough clones also clustered with sequences derived from sand dunes (Kowalchuk et al 1997) and activated sludge (Suwa et al 1997).…”
Section: Diversity Of Ammonia-oxidizing Bacteriasupporting
confidence: 70%
“…The AOB belonging to Sequence Type C are members of Nitrosomonas cluster 6b , Purkhold et al 2000, and their closest cultured relatives are N. marina and N. aestuarii, both halophilic AOB (Pommerening-Röser et al 1996). In the complete absence of salt, members of Nitrosomonas cluster 6a, a typical freshwater cluster consisting of halosensitive bacteria such as N. ureae and N. oligotropha, were enriched (PommereningRöser et al 1996, Speksnijder et al 1998, Purkhold et al 2000. In summary, in the presence of salt, relatives of already known halophilic AOB were enriched, and in its absence, members of a typical freshwater cluster developed.…”
Section: Discussionmentioning
confidence: 99%