Recovery from mild Escherichia coli O157:H7 infection in young and aged C57BL/6 mice with intact flora estimated by fecal shedding, locomotor activity and grip strength

Malavolta, Marco; Basso, Andrea; Giacconi, Robertina; Orlando, Fiorenza; Pierpaoli, Elisa; Cardelli, Maurizio; Leoni, Francesca; Chierichetti, Serena; Bray, Dorothy; Benlhassan, Khadija; Provinciali, Mauro

doi:10.1016/j.cimid.2018.12.003

Cited by 5 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We measured the clinical frailty index (FI) in mice based on the validated murine clinical FI tool described previously [14]. Details on the measurement of weight, body surface temperature and grip strength (which are included in the FI tool) have been also described previously [12]. FI data were recorded the second week of each month from 10 am to 2 pm.…”

Section: Methodsmentioning

confidence: 99%

“…Locomotor activity was conducted by a 5-min open field test on a white wood-chamber (72×72×30 cm) surmounted by a Xiaomi Yi Camera 16MP 1080 P 60FPS (YI Technology) controlled WI-Fi by a Smartphone. Videos were collected in a microSD disk and the tracking was performed offline with Biobserve Viewer3 (Biobserve GmbH, Germany) as previously described [12]. An additional measurement for slowness was obtained by recording the max speed recorded at rotarod test.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

LAV-BPIFB4 associates with reduced frailty in humans and its transfer prevents frailty progression in old mice

Malavolta

Dato

Villa

et al. 2019

Aging

Self Cite

View full text Add to dashboard Cite

Background: There is an increasing concern about age-related frailty because of the growing number of elderly people in the general population. The Longevity-Associated Variant (LAV) of the human BPIFB4 gene was found to correct endothelial dysfunction, one of the mechanisms underlying frailty, in aging mice whereas the RV-BPIFB4 variant induced opposite effects. Thus, we newly hypothesize that, besides being associated with life expectancy, BPIFB4 polymorphisms can predict frailty.Aim and Results: Here we investigated if the BPIFB4 haplotypes, LAV, wild-type (WT) and RV, differentially associate with frailty in a cohort of 237 elderly subjects from Calabria region in Southern Italy. Moreover, we studied the effect of systemic adeno-associated viral vector-mediated LAV-BPIFB4 gene transfer on the progression of frailty in aging mice. We found an inverse correlation of the homozygous LAV-BPIFB4 haplotype with frailty in elderly subjects. Conversely, carriers of the RV-BPIFB4 haplotype showed an increase in the frailty status and risk of death. Moreover, in old mice, LAV-BPIFB4 gene transfer delayed frailty progression.Conclusions: These data indicate that specific BPIFB4 haplotypes could represent useful genetic markers of frailty. In addition, horizontal transfer of a healthy gene variant can attenuate frailty in aging organisms.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

LAV-BPIFB4 associates with reduced frailty in humans and its transfer prevents frailty progression in old mice

Malavolta

Dato

Villa

et al. 2019

Aging

Self Cite

View full text Add to dashboard Cite

show abstract

“…The body length (nose to base of tail) was measured during the locomotor activity test when the mouse was walking straight. The locomotor activity area was calibrated with a ruler, and the mean of 3 length measurements was performed using the video analysis software Tracker (v. [20] with the modification described elsewhere [16,21,22]. To maximally motivate the mice, we used two different sets of weights.…”

Section: Measurement Of Physical Function Score and Frailty Phenotypementioning

confidence: 99%

Comprehensive longitudinal non-invasive quantification of healthspan and frailty in a large cohort (n = 546) of geriatric C57BL/6 J mice

et al. 2023

Self Cite

View full text Add to dashboard Cite

Frailty is an age-related condition characterized by a multisystem functional decline, increased vulnerability to stressors, and adverse health outcomes. Quantifying the degree of frailty in humans and animals is a health measure useful for translational geroscience research. Two frailty measurements, namely the frailty phenotype (FP) and the clinical frailty index (CFI), have been validated in mice and are frequently applied in preclinical research. However, these two tools are based on different concepts and do not necessarily identify the same mice as frail. In particular, the FP is based on a dichotomous classification that suffers from high sample size requirements and misclassification problems. Based on the monthly longitudinal non-invasive assessment of frailty in a large cohort of mice, here we develop an alternative scoring method, which we called physical function score (PFS), proposed as a continuous variable that resumes into a unique function, the five criteria included in the FP. This score would not only reduce misclassification of frailty but it also makes the two tools, PFS and CFI, integrable to provide an overall measurement of health, named vitality score (VS) in aging mice. VS displays a higher association with mortality than PFS or CFI and correlates with

show abstract

“…Escherichia coli is a gram-negative bacterium and common zoonotic pathogen, which causes many human epidemics. In the United States, more than 100,000 people are infected with EHEC 0157:H7 every year (Malavolta et al, 2019). Studies have reported that EHEC infection in pig intestinal tract contents and feces in central China is high (Bae et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial peptide MPX alleviates the lethal attack of Escherichia coli in mice

Zhao

2021

Ukr. Jour. of Vet. and Agr. Sci.

View full text Add to dashboard Cite

Escherichia coli is an important zoonotic pathogen causing intestinal diseases. In recent years, due to the unreasonable use of antibiotics, the drug resistance of bacteria has been increasing, and the proportion of multi-drug resistant strains has also been rising, which directly threatens the health of animals and humans. The antimicrobial peptide MPX was isolated from wasp venom and had better antibacterial activity against Gram-positive and Gram-negative bacteria. Studies have found that MPX had better bactericidal activity against E. coli in vitro. However, whether MPX also has better bactericidal activity in mice is still unknown. This study found that E. coli infected mice lost appetite, diarrhea, and grouping together, while MPX treatment significantly alleviated these symptoms. The autopsy results found that the intestinal congestion, bleeding, thinning of the intestinal wall, yellow viscous fluid in the intestinal cavity, congestion of the lungs, necrosis in the liver, congestion, and bleeding of the spleen, and MPX treatment effectively relieved the above symptoms. The qRT-PCR results found that MPX could increase the mRNA expression of the antibacterial protein TFF3 in the jejunum and colon and reduce the expression of the antibacterial protein Remlβ and REG3γ in the jejunum and colon. H&E staining results further found that MPX could alleviate the pathological damage of mouse intestines and organs caused by E. coli infection. The above results show that MPX has good bactericidal activity against E. coli infection in mice, providing an essential reference for screening drugs for the clinical treatment of E. coli infection.

show abstract

Recovery from mild Escherichia coli O157:H7 infection in young and aged C57BL/6 mice with intact flora estimated by fecal shedding, locomotor activity and grip strength

Cited by 5 publications

References 22 publications

LAV-BPIFB4 associates with reduced frailty in humans and its transfer prevents frailty progression in old mice

LAV-BPIFB4 associates with reduced frailty in humans and its transfer prevents frailty progression in old mice

Comprehensive longitudinal non-invasive quantification of healthspan and frailty in a large cohort (n = 546) of geriatric C57BL/6 J mice

Antimicrobial peptide MPX alleviates the lethal attack of Escherichia coli in mice

Contact Info

Product

Resources

About