Recovery, cryopreservation and fertilization potential of bovine spermatozoa obtained from epididymides stored at 5°C by different periods of time

Martins, Claudia Miranda; Driessen, K.; Costa, P. M.; Carvalho-Neto, J.O.; Sousa, R. V.; Rumpf, R.; Dode, M. A. N.

doi:10.1016/j.anireprosci.2008.12.018

Cited by 49 publications

(67 citation statements)

References 31 publications

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“…In particular, refrigeration temperature induced an increase in total motility of the +51% in the 72R group than in the 72E group. The same condition was observed by other authors in rams at 24 h (Kaabi et al, 2003) and bulls at 48 h of PMT (Martins et al, 2009). As stated in our study, refrigeration had a significant positive effect already after the 24 h of testicle conservation also on the most important sperm kinetic parameters.…”

Section: Discussionsupporting

confidence: 92%

“…However, in this period of time, a decay of total motility and viability, and an increase in abnormal sperm and proximal droplets, were observed. Motility was the variable most affected by PMT as found by other authors in bovine (Martins et al, 2009) andin Ibex (Fernández-Santos et al, 2011). After 48 h of testicle conservation, a significant decrease in total motility and viability occurred, with particular incidence in samples stored at environmental temperature (total motility: −59% from 48E to 72E group, with respect to −30% from 48R to 72R group; viability: −15% from 48E to 72E group, with respect to −8% from 48R to 72R group).…”

Section: Discussionsupporting

confidence: 71%

“…However, sometimes, epididymal sperm extraction is not easily practicable in marginal regions, owing to the lack of facilities and expertise near the farming areas of local breeds. Different studies have been conducted to determine the entity of decay of epididymal spermatozoa quality recovered postmortem in different species, under several experimental conditions, including time elapsed between semen collection and freezing and different testicle storage temperatures (James et al, 2002;Martins et al, 2009;Fernández-Santos et al, 2011). Overall, it has been observed that the resistance over the time of the spermatozoa collected from the testicles is species specific (Killian et al, 2000;Soler et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Turri

Madeddu

Gliozzi

et al. 2014

Animal

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The interest to develop assisted reproductive technologies and cryobanking for farm animal genetic resource conservation has recently increased. However, cryopreservation for ex-situ management of genetic diversity sometimes is not routinely feasible, owing to the lack of facilities (AI centres, laboratories) and expertise near the local breed farming area. In these cases, epididymal sperm obtained from slaughtered or castrated animals, associated with the possibility of managing rather long periods between animal death, sperm recovery and freezing, would increase the opportunities to create semen storages. This investigation addresses the pre-freeze/post-thaw quality of goat epididymal sperm as a function of testicle storage temperature (environment or +5°C) and time elapsed between animal's death and sperm recovery (0, 24, 48, 72 h) to establish the optimal protocols for the recovery and cryopreservation of epididymal sperm in this species. Testicles of 50 mature bucks collected at the abattoir were divided in two groups: half of the testicles (n = 50) were transported to the laboratory at environment temperature (E), whereas the remaining half (n = 50) at a refrigeration temperature (R) of +5°C. In the two groups (E) and (R), one testicle from each pair was processed after slaughter forming the time 0 groups (0E and 0R). The contralateral testicle was processed after 24, 48 or 72 h of storage, at the corresponding temperature. Sperm motility and kinetic parameters, viability and morphology were assessed in pre-freeze and post-thaw samples. Until 48 h postmortem, both E and R temperatures are able to maintain good pre-freeze epididymal sperm quality. After 48 h postmortem, R temperature is fundamental to reduce epididymal sperm quality decay in pre-freeze samples. Moreover, testicle refrigeration also has a positive impact on post-thaw samples, allowing a lower decline through time considering total motility, kinetics parameters, sperm viability and sperm abnormalities. Therefore, when sperm cryopreservation is not immediately practicable, goat testicles should be transported and stored at 5°C up to a maximum of 48 h postmortem to ensure an acceptable sperm quality.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Turri

Madeddu

Gliozzi

et al. 2014

Animal

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show abstract

“…Sin embargo, en este estudio los mejores resultados, se obtuvieron en los grupos uno y dos, dentro de los protocolos evaluados; a las 24h se determinó una viabilidad dentro del protocolo uno (P1) 69±5,8% (G1) y 55±4,3% (G2); 57±4,6% (G1) y 45±4% (G2), en el protocolo 2 (P2) a las 48 h; los grupos 3,4 y 5 no presentaron motilidad individual. Martins et al, (2009) determinó que después de las 48h de almacenamiento en refrigeración, se produce una disminución total de la motilidad, lo que concuerda con los resultados obtenidos en este trabajo, así también lo confirma Anel (2002), mencionando que, dependiendo del intervalo entre la recuperación de los espermatozoides y la muerte, la motilidad espermática disminuirá significativamente entre las 48h.…”

Section: Motilidadunclassified

“…No obstante, el hecho de recuperar espermatozoides de un macho recientemente muerto o sacrificado, implica que existe interés en utilizar sus gametos con fines de conseguir crías en algún momento. Una forma de preservar el germoplasma de estos animales es la colecta de espermatozoides del epidídimo (Martins et al, 2009). Este procedimiento post-mortem es considerado una importante herramienta en la utilización de espermatozoides de animales en peligro de extinción.…”

Section: Introductionunclassified

Evaluación comparativa de dos métodos de recuperación espermática de epidídimos bovinos post-mortem

et al. 2018

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Cryopreservation and its effects on motility and gene expression patterns and fertilizing potential of bovine epididymal sperm

Nazari

Ahmadi

Hamid

et al. 2020

Veterinary Medicine & Sci

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Despite encountering new challenges in using epididymal sperm recovered from cauda epididymides, this accessible and, in some species, worthwhile sample makes inevitable the further development of a suitable cryopreservation protocol. In this study, sperm was recovered from the epididymis of 4°C overnight stored slaughtered bulls' testes and the effects of cryopreservation on the bovine epididymal sperm motility (with CASA) and gene expression patterns (with quantitative Real time‐PCR) were evaluated. Moreover the fertilizing potential of cryopreserved epididymal sperm was used in in vitro fertilization (IVF). After freezing and thawing of epididymal sperm, total and slow progressive sperm motility, VCL, VAP, MAD, ALH and BCF were significantly decreased (p < .05), while in the parameters of fast progressive motility, VSL, LIN, WOB and STR there were not any significant variations in the frozen sperm compared to fresh (non‐frozen) counterpart. The assessment of abundance of transcripts encoding motility (TSSK6) and fertility (PRM1 and PRM2)‐related genes in epididymal sperm, showed that these transcripts were affected by freezing especially in slow progressive motility status (p < .01). Furthermore, cleavage and blastocyst rate did not present any significant differences between bovine embryos produced in vitro by fresh or frozen‐thawed epididymal sperm. It can be concluded that epididymal sperm has enough freezability after overnight testes storage, and cryopreservation could not affect the percentage of in vitro produced embryos in spite of the changes of relative abundance of some transcripts and direction progressive motility pattern of sperm.

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Recovery, cryopreservation and fertilization potential of bovine spermatozoa obtained from epididymides stored at 5°C by different periods of time

Cited by 49 publications

References 31 publications

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Effect of testicle postmortem storage on goat frozen-thawed epididymal sperm quality as a tool to improve genebanking in local breeds

Evaluación comparativa de dos métodos de recuperación espermática de epidídimos bovinos post-mortem

Cryopreservation and its effects on motility and gene expression patterns and fertilizing potential of bovine epididymal sperm

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