Recovering Streptococcus mutans over restorations by the tray technique: a randomized clinical study

Abstract

Aim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge's criteria (examiner´ Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 ºC for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group.

Prevalence and Antibiogram of S. mutans in Dental Plaque and Caries samples

“…In addition, the isolates were able to produce alpha hemolysis on blood agar media as previously confirmed for S. mutans 24 . Furthermore, the colonies showed the characteristic sticky and rough appearance on TYCSB media as showed in the previous study of Vildósola et al 25 . Based on biochemical testing, S. mutans isolates were catalase-negative, able to ferment the sugars mannitol, sorbitol, inulin and sucrose and to change the color of phenol red indicator from red to yellow due to acid production from carbohydrate fermentation which agreed with the studies of Al-Jumaily et al and Vos et al 16,26 .…”

Prevalence and Antibiogram of S. mutans in Dental Plaque and Caries samples

“…In addition, the isolates were able to produce alpha hemolysis on blood agar media as previously confirmed for S. mutans 24 . Furthermore, the colonies showed the characteristic sticky and rough appearance on TYCSB media as showed in the previous study of Vildósola et al 25 . Based on biochemical testing, S. mutans isolates were catalase-negative, able to ferment the sugars mannitol, sorbitol, inulin and sucrose and to change the color of phenol red indicator from red to yellow due to acid production from carbohydrate fermentation which agreed with the studies of Al-Jumaily et al and Vos et al 16,26 .…”

Is there difference of Streptococcus mutans count and adherence on amalgam and resin occlusal restorations? A blind clinical study

“…Microbiological sample: Prior to sampling the biofilm, trays were prepared to print the biofilm over the restorations 17 . For this application, we used disposable fluoride gel application trays (Deepak Products Inc., Miami, USA), each of which was sterilized in a biosafety hood (Esco Technologies Inc., Harboro, USA) under ultraviolet light for 30 minutes.…”

Prevalence and Antibiogram of S. mutans in Dental Plaque and Caries samples

“…In addition, the isolates were able to produce alpha hemolysis on blood agar media as previously confirmed for S. mutans 24 . Furthermore, the colonies showed the characteristic sticky and rough appearance on TYCSB media as showed in the previous study of Vildósola et al 25 . Based on biochemical testing, S. mutans isolates were catalase-negative, able to ferment the sugars mannitol, sorbitol, inulin and sucrose and to change the color of phenol red indicator from red to yellow due to acid production from carbohydrate fermentation which agreed with the studies of Al-Jumaily et al and Vos et al 16,26 .…”

Is there difference of Streptococcus mutans count and adherence on amalgam and resin occlusal restorations? A blind clinical study

“…Microbiological sample: Prior to sampling the biofilm, trays were prepared to print the biofilm over the restorations 17 . For this application, we used disposable fluoride gel application trays (Deepak Products Inc., Miami, USA), each of which was sterilized in a biosafety hood (Esco Technologies Inc., Harboro, USA) under ultraviolet light for 30 minutes.…”

Prevalence and Antibiogram of S. mutans in Dental Plaque and Caries samples

“…In addition, the isolates were able to produce alpha hemolysis on blood agar media as previously confirmed for S. mutans 24 . Furthermore, the colonies showed the characteristic sticky and rough appearance on TYCSB media as showed in the previous study of Vildósola et al 25 . Based on biochemical testing, S. mutans isolates were catalase-negative, able to ferment the sugars mannitol, sorbitol, inulin and sucrose and to change the color of phenol red indicator from red to yellow due to acid production from carbohydrate fermentation which agreed with the studies of Al-Jumaily et al and Vos et al 16,26 .…”

Is there difference of Streptococcus mutans count and adherence on amalgam and resin occlusal restorations? A blind clinical study

“…Microbiological sample: Prior to sampling the biofilm, trays were prepared to print the biofilm over the restorations 17 . For this application, we used disposable fluoride gel application trays (Deepak Products Inc., Miami, USA), each of which was sterilized in a biosafety hood (Esco Technologies Inc., Harboro, USA) under ultraviolet light for 30 minutes.…”